scholarly journals Suprabasin-derived bioactive peptides identified by plasma peptidomics

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tomomi Taguchi ◽  
Yoshio Kodera ◽  
Kazuhito Oba ◽  
Tatsuya Saito ◽  
Yuzuru Nakagawa ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Bioactive Peptides ◽  
Epidermal Differentiation ◽  
Low Molecular Weight ◽  
New Approach ◽  
Liver And Pancreas ◽  
Native Peptides ◽  
Differentiation Marker ◽  
Almost All ◽  
Functional Analyses

AbstractIdentification of low-abundance, low-molecular-weight native peptides using non-tryptic plasma has long remained an unmet challenge, leaving potential bioactive/biomarker peptides undiscovered. We have succeeded in efficiently removing high-abundance plasma proteins to enrich and comprehensively identify low-molecular-weight native peptides using mass spectrometry. Native peptide sequences were chemically synthesized and subsequent functional analyses resulted in the discovery of three novel bioactive polypeptides derived from an epidermal differentiation marker protein, suprabasin. SBSN_HUMAN[279–295] potently suppressed food/water intake and induced locomotor activity when injected intraperitoneally, while SBSN_HUMAN[225–237] and SBSN_HUMAN[243–259] stimulated the expression of proinflammatory cytokines via activation of NF-κB signaling in vascular cells. SBSN_HUMAN[225–237] and SBSN_HUMAN[279–295] immunoreactivities were present in almost all human organs analyzed, while immunoreactive SBSN_HUMAN[243–259] was abundant in the liver and pancreas. Human macrophages expressed the three suprabasin-derived peptides. This study illustrates a new approach for discovering unknown bioactive peptides in plasma via the generation of peptide libraries using a novel peptidomic strategy.

Oil organogel system for magnetorheological fluid

RSC Advances ◽  
2016 ◽  
Vol 6 (114) ◽  
pp. 113463-113468 ◽  
Author(s):  
Jianjian Yang ◽  
Hua Yan ◽  
Hansong Zhang ◽  
Xuemei Wang
Keyword(s):  
Molecular Weight ◽  
Magnetic Particles ◽  
Magnetorheological Fluid ◽  
Low Molecular Weight ◽  
New Approach ◽  
Low Molecular Weight Gelator

A new approach to dispersing magnetic particles via an oil organogel formed by a low molecular weight gelator to prepare MRF.

scholarly journals Bioactive Properties of Breads Made with Sourdough of Hull-Less Barley or Conventional and Pigmented Wheat Flours

2021 ◽  
Vol 11 (7) ◽  
pp. 3291
Author(s):  
Simone Luti ◽  
Viola Galli ◽  
Manuel Venturi ◽  
Lisa Granchi ◽  
Paolo Paoli ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Bioactive Peptides ◽  
Antioxidant Compounds ◽  
Low Molecular Weight ◽  
Beneficial Effects ◽  
Baked Goods ◽  
Inflammatory Status ◽  
Anti Inflammatory ◽  
Stressed Cells ◽  
Pathway Inhibition

Functional and nutritional properties of baked goods can be enhanced by the use of sourdough fermentation, which is defined as a mixture of water and flour fermented by lactobacilli (LAB) and yeasts. Previous data highlighted the ability of sourdoughs obtained with selected LAB strains and commercial flour to produce bioactive peptides equipped with antioxidant and anti-inflammatory properties. More recently, it has been proven that choosing the most suitable combination of selected LAB and high-functional-value flours greatly increase the amount of low-molecular-weight antioxidant compounds responsible for improving the nutritional value of the products. This work aimed to isolate bioactive peptides both from sourdoughs and baked breads obtained with pigmented wheat and barley and a selected combination of LABs. Soluble water extracts were obtained, and low-molecular-weight peptides were isolated. Antioxidant activity was detected by assaying the intracellular ROS production in stressed cultured macrophages, treated with peptides. Moreover, anti-inflammatory activity, highlighted by NFkB pathway inhibition and by COX2 reduction in stressed cells, was demonstrated for peptides obtained from breads. The results allowed the conclusion that the combination of flours and LAB used in the present manuscript led to the production of bakery products with beneficial effects on oxidative and inflammatory status.

Radioiodination of Alginate via Covalently-Bound Tyrosinamide Allows Monitoring of its Fate In Vivo

1995 ◽  
Vol 10 (1) ◽  
pp. 4-13 ◽  
Author(s):  
Aymen Al-Shamkhani ◽  
Ruth Duncan
Keyword(s):  
Molecular Weight ◽  
Intraperitoneal Injection ◽  
Specific Activity ◽  
Subcutaneous Administration ◽  
High Specific Activity ◽  
Weight Fraction ◽  
Low Molecular Weight ◽  
Blood Compartment ◽  
Almost All

Tb monitor the fate of alginate following systemic administration, a method was developed that allowed the covalent incorporation of approximately 1 mol% tyrosinamide. The product could be radioiodinated to a high specific activity, and was subsequently stable on storage at 4°C for 30 days, with very little (c 1%) free [125I] iodide released over that period. Twenty-four hours following intravenous administration, the low molecular weight fraction (<48,000) of the injected polymer was excreted in the urine while the larger polymer fraction remained in the circulation and did not readily accumulate in any of the tissues. Almost all of the dose administered by intraperitoneal injection was transferred from the peritoneal cavity to the blood compartment within 24 h, with the low molecular weight fraction of the polymer excreted in the urine. Following subcutaneous administration, the majority (-70%) of the injected dose was retained at the site of injection at 24 h.

scholarly journals Aggregation, Sedimentation, and Dissolution of Copper Oxide Nanoparticles: Influence of Low-Molecular-Weight Organic Acids from Root Exudates

Nanomaterials ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 841 ◽  
Author(s):  
Cheng Peng ◽  
Hong Tong ◽  
Peng Yuan ◽  
Lijuan Sun ◽  
Lei Jiang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Organic Acids ◽  
Root Exudates ◽  
Copper Oxide Nanoparticles ◽  
Rice Root ◽  
Low Molecular Weight ◽  
Plant Interactions ◽  
Cuo Nps ◽  
The Individual ◽  
Almost All

The rhizosphere is an essential pathway for the uptake of metal-based nanoparticles (MNPs) by plant roots. However, the interaction between root exudates and MNPs is still unclear. In this study, we initially identified the major low-molecular-weight organic acids (LMWOAs) in the rice root exudates using hydroponics. Then, the individual LMWOAs were added to CuO nanoparticle suspensions to investigate their effects on the environmental behavior of the MNPs. The results showed that both the variety and the concentration of LMWOAs impacted the aggregation, sedimentation, and dissolution of CuO nanoparticles (NPs). Almost all LMWOAs except succinic acid inhibited the aggregation of CuO NPs by enhancing the electrostatic repulsive force between NPs. The presence of citric and oxalic acids rather than lactic acid greatly improved the stability of CuO NP suspensions, but other acids showed a low promoting and high inhibiting effect on NP sedimentation. Moreover, all the LMWOAs from root exudates facilitated the dissolution of CuO NPs with a positive dose-dependent correlation, especially formic acid. Notably, citric acid, as the most abundant LMWOAs in rice root exudates, largely determined the aggregation, sedimentation, and dissolution of CuO NPs. This study provides a better understanding on NP–plant interactions in the rhizosphere.

A new approach for prolonging the half-life of peptides, proteins and low-molecular-weight drugs in vivo

2001 ◽  
Vol 26 (7) ◽  
pp. 669 ◽  
Author(s):  
Y. Shechter ◽  
I. Goldwaser ◽  
I. Lavon ◽  
E. Gershonov ◽  
B. Mester ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Half Life ◽  
Low Molecular Weight ◽  
New Approach
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