scholarly journals Pseudogymnoascus destructans growth in wood, soil and guano substrates

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jenny Urbina ◽  
Tara Chestnut ◽  
Jennifer M. Allen ◽  
Taal Levi
Keyword(s):  
Dna Extraction ◽  
Emerging Infectious Diseases ◽  
Transmission Risk ◽  
Pseudogymnoascus Destructans ◽  
White Nose Syndrome ◽  
Management Interventions ◽  
Pathogen Persistence ◽  
Tenfold Increase ◽  
And Control

AbstractUnderstanding how a pathogen can grow on different substrates and how this growth impacts its dispersal are critical to understanding the risks and control of emerging infectious diseases. Pseudogymnoascus destructans (Pd) causes white-nose syndrome (WNS) in many bat species and can persist in, and transmit from, the environment. We experimentally evaluated Pd growth on common substrates to better understand mechanisms of pathogen persistence, transmission and viability. We inoculated autoclaved guano, fresh guano, soil, and wood with live Pd fungus and evaluated (1) whether Pd grows or persists on each (2) if spores of the fungus remain viable 4 months after inoculation on each substrate, and (3) whether detection and quantitation of Pd on swabs is sensitive to the choice to two commonly used DNA extraction kits. After inoculating each substrate with 460,000 Pd spores, we collected ~ 0.20 g of guano and soil, and swabs from wood every 16 days for 64 days to quantify pathogen load through time using real-time qPCR. We detected Pd on all substrates over the course of the experiment. We observed a tenfold increase in pathogen loads on autoclaved guano and persistence but not growth in fresh guano. Pathogen loads increased marginally on wood but declined ~ 60-fold in soil. After four months, apparently viable spores were harvested from all substrates but germination did not occur from fresh guano. We additionally found that detection and quantitation of Pd from swabs of wood surfaces is sensitive to the DNA extraction method. The commonly used PrepMan Ultra Reagent protocol yielded substantially less DNA than did the QIAGEN DNeasy Blood and Tissue Kit. Notably the PrepMan Ultra Reagent failed to detect Pd in many wood swabs that were detected by QIAGEN and were subsequently found to contain substantial live conidia. Our results indicate that Pd can persist or even grow on common environmental substrates with results dependent on whether microbial competitors have been eliminated. Although we observed clear rapid declines in Pd on soil, viable spores were harvested four months after inoculation. These results suggest that environmental substrates and guano can in general serve as infectious environmental reservoirs due to long-term persistence, and even growth, of live Pd. This should inform management interventions to sanitize or modify structures to reduce transmission risk as well early detection rapid response (EDRR) planning.

scholarly journals Sex and hibernaculum temperature predict survivorship in white-nose syndrome affected little brown myotis ( Myotis lucifugus )

2015 ◽  
Vol 2 (2) ◽  
pp. 140470 ◽  
Author(s):  
Laura E. Grieneisen ◽  
Sarah A. Brownlee-Bouboulis ◽  
Joseph S. Johnson ◽  
DeeAnn M. Reeder
Keyword(s):  
Infectious Disease ◽  
Myotis Lucifugus ◽  
Eastern North America ◽  
The Novel ◽  
Pseudogymnoascus Destructans ◽  
Term Survival ◽  
White Nose Syndrome ◽  
Myotis Myotis ◽  
The Impact

White-nose syndrome (WNS), an emerging infectious disease caused by the novel fungus Pseudogymnoascus destructans , has devastated North American bat populations since its discovery in 2006. The little brown myotis, Myotis lucifugus , has been especially affected. The goal of this 2-year captive study was to determine the impact of hibernacula temperature and sex on WNS survivorship in little brown myotis that displayed visible fungal infection when collected from affected hibernacula. In study 1, we found that WNS-affected male bats had increased survival over females and that bats housed at a colder temperature survived longer than those housed at warmer temperatures. In study 2, we found that WNS-affected bats housed at a colder temperature fared worse than unaffected bats. Our results demonstrate that WNS mortality varies among individuals, and that colder hibernacula are more favourable for survival. They also suggest that female bats may be more negatively affected by WNS than male bats, which has important implications for the long-term survival of the little brown myotis in eastern North America.

scholarly journals The dynamics of nematode infections of farmed ruminants

Parasitology ◽  
1995 ◽  
Vol 110 (4) ◽  
pp. 493-502 ◽  
Author(s):  
M. G. Roberts ◽  
J. A. P. Heesterbeek
Keyword(s):  
Parasite Species ◽  
Equation Model ◽  
Host Population ◽  
Transition Rates ◽  
Differential Equation Model ◽  
Nematode Parasites ◽  
Management Interventions ◽  
Dynamics And Control ◽  
And Control

SUMMARYIn this paper the dynamics and control of nematode parasites of farmed ruminants are discussed via a qualitative analysis of a differential equation model. To achieve this a quantity, ‘the basic reproduction quotient’ (Q0), whose definition coincides with previous definitions of R0 for macroparasites, but extends to models with periodic time-varying transition rates between parasite stages or management interventions, is introduced. This quantity has the usual threshold property: if Q0 is less than one the parasite population cannot maintain itself in the host population, and in the long term becomes extinct; but if Q0 is greater than one the parasite can invade the host population. An alternative quantity, R(E), that is often easier to calculate is also introduced, and shown to have the same threshold property. The use of these two quantities in analysing models for the dynamics of nematodes in complex situations is then demonstrated, with reference to the dynamics of mixed parasite species in one host; the effects of breeding host animals for resistance to parasitism; and the development of parasite strains that are resistant to chemotherapy. Five examples are discussed using parameters for the dynamics of nematode infections in sheep, and some statements on control policies are derived.

Long-Term Persistence of Pseudogymnoascus destructans, the Causative Agent of White-Nose Syndrome, in the Absence of Bats

EcoHealth ◽  
2014 ◽  
Vol 12 (2) ◽  
pp. 330-333 ◽  
Author(s):  
Joseph R. Hoyt ◽  
Kate E. Langwig ◽  
Joseph Okoniewski ◽  
Winifred F. Frick ◽  
Ward B. Stone ◽  
...  
Keyword(s):  
Causative Agent ◽  
Pseudogymnoascus Destructans ◽  
White Nose Syndrome

scholarly journals Observed Resiliency of Little Brown Myotis to Long-Term White-Nose Syndrome Exposure

2018 ◽  
Vol 9 (1) ◽  
pp. 168-179 ◽  
Author(s):  
Christopher A. Dobony ◽  
Joshua B. Johnson
Keyword(s):  
Survival Rates ◽  
Reproductive Potential ◽  
Myotis Lucifugus ◽  
Recent Past ◽  
Pseudogymnoascus Destructans ◽  
Recent Infection ◽  
Term Survival ◽  
Annual Survival ◽  
White Nose Syndrome

Abstract White-nose syndrome (WNS) is a disease that has killed millions of bats in eastern North America and has steadily been spreading across the continent. Little brown myotis Myotis lucifugus populations have experienced extensive declines; however, some localized populations have remained resilient, with bats surviving multiple years past initial WNS exposure. These persistent populations may be critical to species recovery, and understanding mechanisms leading to this long-term survival and persistence may provide insight into overall bat and disease management. We monitored a maternity colony of little brown myotis on Fort Drum Military Installation in northern New York between 2006 and 2017 to determine basic demographic parameters and find evidence of what may be leading to resiliency and persistence at this site. Total colony size declined by approximately 88% from 2008 to 2010 due primarily to impacts of WNS. Counts of all adults returning to the colony stabilized during 2010–2014 (mean = 94, range 84–101) and increased after 2014 (mean = 132, range = 108–166). We captured 727 little brown myotis (575 females, 152 males) and banded 534 individuals (389 females, 145 males) at the colony. The majority of sampled bats showed evidence of recent past WNS infection and exposure to Pseudogymnoascus destructans, and we documented pervasive presence and limited viability of the fungus within the colony's main roosting structure. We recaptured 98 individually marked females in years after initial banding, and some individuals survived at least 6 y. Ninety-one percent of all adult females, 93% of recaptured bats, and 90% of 1-y-old females (i.e., bats recaptured the first year after initial capture as juveniles) showed evidence of reproduction during the monitoring period. Using mark–recapture models, we estimated annual survival rates of juvenile and adult little brown myotis during 2009–2016 and examined whether reproductive condition or evidence of recent infection of WNS had any effect on survival. Annual survival rates were similar between juveniles and adults, but highly variable, ranging from 41.0 to 86.5%. Models indicated that neither evidence of recent past exposure to WNS nor reproductive status were related to survival. No one parameter stood out as being responsible for this colony's continued existence, and it is likely that many interwoven factors were responsible for the observed resilience. Although relatively high reproductive effort from all females (i.e., both1-y-old and >1-y-old ) and intermittently suitable survival rates have led to the continued persistence of, and population increases in, this summer colony, mortality from WNS and inherently low reproductive potential still seemed to be limiting population growth. Until there is a better understanding of this overall potential resiliency in little brown myotis, we recommend considering minimizing disturbance and direct human involvement within these persisting populations to allow whatever natural recovery that may be occurring to evolve uninterrupted.

Suppressed, but Not Forgotten

2011 ◽  
Vol 70 (1) ◽  
pp. 5-11 ◽  
Author(s):  
Beat Meier ◽  
Anja König ◽  
Samuel Parak ◽  
Katharina Henke
Keyword(s):  
Memory Trace ◽  
Thought Suppression ◽  
Test Phase ◽  
Indirect Test ◽  
Final Test ◽  
Test Experiment ◽  
And Control ◽  
Cue Words ◽  
The Impact

This study investigates the impact of thought suppression over a 1-week interval. In two experiments with 80 university students each, we used the think/no-think paradigm in which participants initially learn a list of word pairs (cue-target associations). Then they were presented with some of the cue words again and should either respond with the target word or avoid thinking about it. In the final test phase, their memory for the initially learned cue-target pairs was tested. In Experiment 1, type of memory test was manipulated (i.e., direct vs. indirect). In Experiment 2, type of no-think instructions was manipulated (i.e., suppress vs. substitute). Overall, our results showed poorer memory for no-think and control items compared to think items across all experiments and conditions. Critically, however, more no-think than control items were remembered after the 1-week interval in the direct, but not in the indirect test (Experiment 1) and with thought suppression, but not thought substitution instructions (Experiment 2). We suggest that during thought suppression a brief reactivation of the learned association may lead to reconsolidation of the memory trace and hence to better retrieval of suppressed than control items in the long term.

Strengthening of occupational health promotion by the German Prevention Health Care Act

2019 ◽  
pp. 188-190
Author(s):  
Diana Hart
Keyword(s):  
Public Health ◽  
Health Care ◽  
Health Promotion ◽  
Primary Prevention ◽  
Occupational Health ◽  
Life Expectancy ◽  
Prevention And Control ◽  
Occupational Health Promotion ◽  
And Control

All countries are faced with the problem of the prevention and control of non-communicable diseases (NCD): implement prevention strategies eff ectively, keep up the momentum with long term benefi ts at the individual and the population level, at the same time tackling hea lth inequalities. Th e aff ordability of therapy and care including innovative therapies is going to be one of the key public health priorities in the years to come. Germany has taken in the prevention and control of NCDs. Germany’s health system has a long history of guaranteeing access to high-quality treatment through universal health care coverage. Th r ough their membership people are entitled to prevention and care services maintaining and restoring their health as well as long term follow-up. Like in many other countries general life expectancy has been increasing steadily in Germany. Currently, the average life expectancy is 83 and 79 years in women and men, respectively. Th e other side of the coin is that population aging is strongly associated with a growing burden of disease from NCDs. Already over 70 percent of all deaths in Germany are caused by four disease entities: cardiovascular disease, cancer, chronic respiratory disease and diabetes. Th ese diseases all share four common risk factors: smoking, alcohol abuse, lack of physical activity and overweight. At the same time, more and more people become long term survivors of disease due to improved therapy and care. Th e German Government and public health decision makers are aware of the need for action and have responded by initiating and implementing a wide spectrum of activities. One instrument by strengthening primary prevention is the Prevention Health Care Act. Its overarching aim is to prevent NCDs before they can manifest themselves by strengthening primary prevention and health promotion in diff erent sett ings. One of the main emphasis of the Prevention Health Care Act is the occupational health promotion at the workplace.

Cognitive Treatment for OCD

2012 ◽  
Author(s):  
Maureen L. Whittal ◽  
Melisa Robichaud
Keyword(s):  
Term Treatment ◽  
Future Directions ◽  
Cognitive Treatment ◽  
Long Term Treatment ◽  
Inflated Responsibility ◽  
And Control ◽  
The Relationship ◽  
Control Of Thoughts

The cornerstone of cognitive treatment (CT) for OCD is based upon the knowledge that unwanted intrusions are essentially a universal experience. As such, it is not the presence of the intrusion that is problematic but rather the associated meaning or interpretation. Treatment is flexible, depending upon the nature of the appraisals and beliefs, but can include strategies focused on inflated responsibility and overestimation of threat, importance and control of thoughts, and the need for perfectionism and certainty. The role of concealment and the relationship to personal values are important maintaining and etiological factors. The short-term and long-term treatment outcome is reviewed, along with predictors of treatment response and mechanisms of action, and the chapter concludes with future directions regarding CT for OCD.

scholarly journals The Anti-PD-1/PD-L1 Immunotherapy for Gastric Esophageal Cancer: A Systematic Review and Meta-Analysis and Literature Review

2021 ◽  
Vol 28 ◽  
pp. 107327482199743
Author(s):  
Ke Chen ◽  
Xiao Wang ◽  
Liu Yang ◽  
Zheling Chen
Keyword(s):  
Overall Survival ◽  
Esophageal Cancer ◽  
Survival Rate ◽  
Meta Analysis ◽  
Response Rates ◽  
Control Group ◽  
Term Survival ◽  
Long Term Survival ◽  
And Control

Background: Treatment options for advanced gastric esophageal cancer are quite limited. Chemotherapy is unavoidable at certain stages, and research on targeted therapies has mostly failed. The advent of immunotherapy has brought hope for the treatment of advanced gastric esophageal cancer. The aim of the study was to analyze the safety of anti-PD-1/PD-L1 immunotherapy and the long-term survival of patients who were diagnosed as gastric esophageal cancer and received anti-PD-1/PD-L1 immunotherapy. Method: Studies on anti-PD-1/PD-L1 immunotherapy of advanced gastric esophageal cancer published before February 1, 2020 were searched online. The survival (e.g. 6-month overall survival, 12-month overall survival (OS), progression-free survival (PFS), objective response rates (ORR)) and adverse effects of immunotherapy were compared to that of control therapy (physician’s choice of therapy). Results: After screening 185 studies, 4 comparative cohort studies which reported the long-term survival of patients receiving immunotherapy were included. Compared to control group, the 12-month survival (OR = 1.67, 95% CI: 1.31 to 2.12, P < 0.0001) and 18-month survival (OR = 1.98, 95% CI: 1.39 to 2.81, P = 0.0001) were significantly longer in immunotherapy group. The 3-month survival rate (OR = 1.05, 95% CI: 0.36 to 3.06, P = 0.92) and 18-month survival rate (OR = 1.44, 95% CI: 0.98 to 2.12, P = 0.07) were not significantly different between immunotherapy group and control group. The ORR were not significantly different between immunotherapy group and control group (OR = 1.54, 95% CI: 0.65 to 3.66, P = 0.01). Meta-analysis pointed out that in the PD-L1 CPS ≥10 sub group population, the immunotherapy could obviously benefit the patients in tumor response rates (OR = 3.80, 95% CI: 1.89 to 7.61, P = 0.0002). Conclusion: For the treatment of advanced gastric esophageal cancer, the therapeutic efficacy of anti-PD-1/PD-L1 immunotherapy was superior to that of chemotherapy or palliative care.

scholarly journals Preventing and controlling water pipe smoking: a systematic review of management interventions

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Javad Babaie ◽  
Ayat Ahmadi ◽  
Gholamreza Abdollahi ◽  
Leila Doshmangir
Keyword(s):  
Data Extraction ◽  
Water Pipe ◽  
Health Crisis ◽  
Adverse Health Effects ◽  
Analysis Process ◽  
School Based ◽  
Management Interventions ◽  
School Based Interventions ◽  
Global Arena ◽  
And Control

Abstract Background Water pipe smoking (WPS) is re-gaining widespread use and popularity among various groups of people, especially adolescents. Despite different adverse health effects of WPS, many of the WPS interventions have failed to control this type of tobacco smoking. This study was conducted to identify experienced management interventions in preventing and controlling WPS worldwide. Methods A systematic literature review was conducted. Electronic databases were searched for recordes which were published from beginning 1990 to August 2018. Studies aiming at evaluating, at least, one intervention in preventing and controlling WPS were included in this review, followed by performing the quality assessment and data extraction of eligible studies by two independent investigators. Finally, interventions that were identified from the content analysis process were discussed and classified into relevant categories. Results After deleting duplications, 2228 out of 4343 retrieved records remained and 38 studies were selected as the main corpus of the present study. Then, the identified 27 interventions were grouped into four main categories including preventive (5, 18.51%) and control (8, 29.62%) interventions, as well as the enactment and implementation of legislations and policies for controlling WPS at national (7, 25.92%) and international (7, 25.92%) levels. Conclusion The current enforced legislations for preventing and controlling WPS are not supported by rigorous evidence. Informed school-based interventions, especially among adolescents can lead to promising results in preventing and controlling WPS and decreasing the effects of this important social and health crisis in the global arena.

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