scholarly journals Publisher Correction: A first experience of transduction for differentiated HepaRG cells using lentiviral technology

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Adeline Pivert ◽  
Caroline Lefeuvre ◽  
Cong-Tri Tran ◽  
Claude Baillou ◽  
David Durantel ◽  
...  
Keyword(s):  
Heparg Cells

scholarly journals Comparative Proteomics Reveals Novel Components at the Plasma Membrane of Differentiated HepaRG Cells and Different Distribution in Hepatocyte- and Biliary-Like Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e71859 ◽  
Author(s):  
Catalina Petrareanu ◽  
Alina Macovei ◽  
Izabela Sokolowska ◽  
Alisa G. Woods ◽  
Catalin Lazar ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Comparative Proteomics ◽  
Heparg Cells

Cholestatic drugs impair bile acid profiles and disposition in HepaRG cells

2016 ◽  
Vol 258 ◽  
pp. S55
Author(s):  
A. Sharanek ◽  
A. Burban ◽  
L. Humbert ◽  
D. Rainteau ◽  
A. Guillouzo
Keyword(s):  
Bile Acid ◽  
Heparg Cells ◽  
Bile Acid Profiles

Pesticide mixture effects on liver protein abundance in HepaRG cells

Toxicology ◽  
2021 ◽  
pp. 152839
Author(s):  
Felix F. Schmidt ◽  
Dajana Lichtenstein ◽  
Hannes Planatscher ◽  
Almut Mentz ◽  
Joern Kalinowski ◽  
...  
Keyword(s):  
Protein Abundance ◽  
Liver Protein ◽  
Pesticide Mixture ◽  
Mixture Effects ◽  
Heparg Cells

scholarly journals Magnesium Isoglycyrrhizinate Reduces Hepatic Lipotoxicity through Regulating Metabolic Abnormalities

2021 ◽  
Vol 22 (11) ◽  
pp. 5884
Author(s):  
Li Lu ◽  
Kun Hao ◽  
Yu Hong ◽  
Jie Liu ◽  
Jinwei Zhu ◽  
...  
Keyword(s):  
Metabolic Diseases ◽  
Lipid Synthesis ◽  
Total Lipid Content ◽  
Metabolic Activation ◽  
Metabolite Profile ◽  
Metabolic Enzymes ◽  
Antagonistic Effect ◽  
Heparg Cells ◽  
Hepatic Lipotoxicity ◽  
Magnesium Isoglycyrrhizinate

The excessive accumulation of lipids in hepatocytes induces a type of cytotoxicity called hepatic lipotoxicity, which is a fundamental contributor to liver metabolic diseases (such as NAFLD). Magnesium isoglycyrrhizinate (MGIG), a magnesium salt of the stereoisomer of natural glycyrrhizic acid, is widely used as a safe and effective liver protectant. However, the mechanism by which MGIG protects against NAFLD remains unknown. Based on the significant correlation between NAFLD and the reprogramming of liver metabolism, we aimed to explore the beneficial effects of MGIG from a metabolic viewpoint in this paper. We treated HepaRG cells with palmitic acid (PA, a saturated fatty acid of C16:0) to induce lipotoxicity and then evaluated the antagonistic effect of MGIG on lipotoxicity by investigating the cell survival rate, DNA proliferation rate, organelle damage, and endoplasmic reticulum stress (ERS). Metabolomics, lipidomics, and isotope tracing were used to investigate changes in the metabolite profile, lipid profile, and lipid flux in HepaRG cells under different intervention conditions. The results showed that MGIG can indeed protect hepatocytes against PA-induced cytotoxicity and ERS. In response to the metabolic abnormality of lipotoxicity, MGIG curtailed the metabolic activation of lipids induced by PA. The content of total lipids and saturated lipids containing C16:0 chains increased significantly after PA stimulation and then decreased significantly or even returned to normal levels after MGIG intervention. Lipidomic data show that glycerides and glycerophospholipids were the two most affected lipids. For excessive lipid accumulation in hepatocytes, MGIG can downregulate the expression of the metabolic enzymes (GPATs and DAGTs) involved in triglyceride biosynthesis. In conclusion, MGIG has a positive regulatory effect on the metabolic disorders that occur in hepatocytes under lipotoxicity, and the main mechanisms of this effect are in lipid metabolism, including reducing the total lipid content, reducing lipid saturation, inhibiting glyceride and glycerophospholipid metabolism, and downregulating the expression of metabolic enzymes in lipid synthesis.

A novel prediction model to evaluate genotoxicity based on a gene signature in metabolically competent human HepaRG? cells

2021 ◽  
Vol 350 ◽  
pp. S66
Author(s):  
A. Thienpont ◽  
S. Verhulst ◽  
L. van Grunsven ◽  
V. Rogiers ◽  
T Vanhaecke ◽  
...  
Keyword(s):  
Prediction Model ◽  
Gene Signature ◽  
Heparg Cells

Comparison of Cryopreserved HepaRG Cells with Cryopreserved Human Hepatocytes for Prediction of Clearance for 26 Drugs

2011 ◽  
Vol 40 (1) ◽  
pp. 104-110 ◽  
Author(s):  
Ugo Zanelli ◽  
Nicola Pasquale Caradonna ◽  
David Hallifax ◽  
Elisa Turlizzi ◽  
J. Brian Houston
Keyword(s):  
Human Hepatocytes ◽  
Heparg Cells

Cholestatic drugs induce preferential accumulation of toxic bile acids in human HepaRG cells

2017 ◽  
Vol 280 ◽  
pp. S225
Author(s):  
Ahmad Sharanek ◽  
Audrey Burban ◽  
Lydie Humbert ◽  
Christiane Guguen-Guillouzo ◽  
Dominique Rainteau ◽  
...  
Keyword(s):  
Bile Acids ◽  
Preferential Accumulation ◽  
Heparg Cells

scholarly journals Generation of functional cholangiocyte‐like cells from human pluripotent stem cells and HepaRG cells

Hepatology ◽  
2014 ◽  
Vol 60 (2) ◽  
pp. 700-714 ◽  
Author(s):  
Noushin Dianat ◽  
Hélène Dubois‐Pot‐Schneider ◽  
Clara Steichen ◽  
Christophe Desterke ◽  
Philippe Leclerc ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Heparg Cells

scholarly journals Studies on the Role of Metabolic Activation in Tyrosine Kinase Inhibitor–Dependent Hepatotoxicity: Induction of CYP3A4 Enhances the Cytotoxicity of Lapatinib in HepaRG Cells

2013 ◽  
Vol 42 (1) ◽  
pp. 162-171 ◽  
Author(s):  
Klarissa D. Hardy ◽  
Michelle D. Wahlin ◽  
Ioannis Papageorgiou ◽  
Jashvant D. Unadkat ◽  
Allan E. Rettie ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
Tyrosine Kinase Inhibitor ◽  
Kinase Inhibitor ◽  
Metabolic Activation ◽  
Heparg Cells
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