scholarly journals Combined radiation and p53 gene therapy of malignant glioma cells

1999 ◽  
Vol 6 (2) ◽  
pp. 155-162 ◽  
Author(s):  
Behnam Badie ◽  
Chern Sing Goh ◽  
Jessica Klaver ◽  
Hans Herweijer ◽  
David A Boothman
Keyword(s):  
Gene Therapy ◽  
Malignant Glioma ◽  
Glioma Cells ◽  
P53 Gene ◽  
Malignant Glioma Cells

Targeting adenovirus to CD80 and CD86 receptors increases gene transfer efficiency to malignant glioma cells

2007 ◽  
Vol 107 (3) ◽  
pp. 617-627 ◽  
Author(s):  
Ilya V. Ulasov ◽  
Angel A. Rivera ◽  
Yu Han ◽  
David T. Curiel ◽  
Zeng B. Zhu ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Brain Tumors ◽  
Malignant Glioma ◽  
Malignant Gliomas ◽  
Glioma Cells ◽  
Luciferase Reporter ◽  
Transduction Efficiency ◽  
Wild Type ◽  
Malignant Glioma Cells

Object Gene therapy protocols for malignant gliomas utilize adenoviral vectors that rely almost exclusively on the adenovirus serotype 5 (Ad5) backbone. The authors have previously shown that chimeric vectors that bind to the Ad3 receptor, or CD46, increase the transduction efficiency of malignant brain tumors. In light of the debate regarding the efficacy of CD46 compared with CD80/CD86 in binding Ad3 virions, the authors now examine the expression and transduction efficiency of Ad5/3 chimeras that bind via CD80/CD86. Methods The authors first analyzed CD80/CD86 expression in glioma cell lines. They then used three replication-defective vectors containing a luciferase reporter gene: Ad5/3 (containing the tail and shaft domain of Ad5 and the knob domain of Ad3); Ad3/5 (containing the tail of Ad5, shaft of Ad3, and knob of Ad5); and Ad3/3 (containing the tail of Ad5, shaft of Ad3, and knob of Ad3). These vectors were analyzed both in vitro and in vivo against malignant glioma cells. To examine further the effect of Ad5/3 fiber modification, the authors created an oncolytic vector, conditionally replicative Ad5/3 (CRAd5/3). Results The Ad5/3 vector showed a 10- to 100-fold enhanced transduction efficiency of malignant glioma compared with replication-defective wild-type adenovirus (reAd5) (p < 0.05). Moreover the use of Ad5/3 reduced transgene expression by more than 90% in normal human brain cells compared with reAd5. Finally, the use of CRAd5/3 inhibited tumor cell proliferation by 43% more than replication-competent wild-type virus in vitro (p < 0.05). Conclusions The results of this study demonstrate that the Ad5/3 vector offers superior transduction efficiency and low toxicity in the setting of brain tumors, and therefore represents a potential new approach to gene therapy for malignant gliomas.

scholarly journals Synthesis and biological effects of NO in malignant glioma cells: modulation by cytokines including CD95L and TGF-β dexamethasone, and p53 gene transfer

Oncogene ◽  
1998 ◽  
Vol 17 (18) ◽  
pp. 2323-2332 ◽  
Author(s):  
Johannes Rieger ◽  
Marko Ständer ◽  
Peter A Löschmann ◽  
Michael Heneka ◽  
Johannes Dichgans ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Malignant Glioma ◽  
Biological Effects ◽  
Glioma Cells ◽  
P53 Gene ◽  
Malignant Glioma Cells
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