Modulation properties of factors released by bone marrow stromal cells on activated microglia: an in vitro study

Bone remodelling is a coupled process of bone formation and resorption. This process is physiologically controlled and involves the synthesis of bone matrix by osteoblasts and bone resorption by giant cells called osteoclasts. It includes a complex interaction of cells, with specific chemokine signalling. In order to study bone remodelling in vitro, we cultivated two precursor cellline types in a model coculture system on synthetic hydroxyapatite ceramic (HA) for 14 days. The monocytes have the capacity to differentiate into osteoclast-like cells, and the bone marrow stromal cells can differentiate into osteoblast-like cells. This coculture was used to analyse the in vitro cell interaction between monocytes and stromal cells. Furthermore, the attachment of the bone marrow stromal cells to the resorbed HA-surfaces was studied. In this in vitro study we demonstrated osteoclast-like differentiation and bone marrow stromal growth in a coculture system on a synthetic bone substitute material. Under optimal conditions, HA can be resorbed and bone marrow cells can grow into the lacunas to form new bone. These results give important cues for the adjustment of synthetic bone substitute materials for optimal remodelling behaviour.

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A Method for Measurement of Drug Sensitivity of Myeloma Cells Co-Cultured with Bone Marrow Stromal Cells

CrossRef Listing of Deleted DOIs ◽

10.1177/1087057113478168 ◽

2013 ◽

Vol 18 (6) ◽

pp. 637-646 ◽

Cited By ~ 18

Author(s):

Kristine Misund ◽

Katarzyna A. Baranowska ◽

Toril Holien ◽

Christoph Rampa ◽

Dionne C. G. Klein ◽

...

Keyword(s):

Bone Marrow ◽

Cell Survival ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Large Scale ◽

Stromal Cell ◽

Marrow Stromal Cells ◽

Cell Nuclei ◽

Myeloma Cells

The tumor microenvironment can profoundly affect tumor cell survival as well as alter antitumor drug activity. However, conventional anticancer drug screening typically is performed in the absence of stromal cells. Here, we analyzed survival of myeloma cells co-cultured with bone marrow stromal cells (BMSC) using an automated fluorescence microscope platform, ScanR. By staining the cell nuclei with DRAQ5, we could distinguish between BMSC and myeloma cells, based on their staining intensity and nuclear shape. Using the apoptotic marker YO-PRO-1, the effects of drug treatment on the viability of the myeloma cells in the presence of stromal cells could be measured. The method does not require cell staining before incubation with drugs, and less than 5000 cells are required per condition. The method can be used for large-scale screening of anticancer drugs on primary myeloma cells. This study shows the importance of stromal cell support for primary myeloma cell survival in vitro, as half of the cell samples had a marked increase in their viability when cultured in the presence of BMSC. Stromal cell–induced protection against common myeloma drugs is also observed with this method.

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