scholarly journals Bacillus cereus strain S2 shows high nematicidal activity against Meloidogyne incognita by producing sphingosine

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Huijuan Gao ◽  
Gaofu Qi ◽  
Rong Yin ◽  
Hongchun Zhang ◽  
Chenggang Li ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Meloidogyne Incognita ◽  
Nematicidal Activity

Volatile organic compounds of Bacillus cereus strain Bc-cm103 exhibit fumigation activity against Meloidogyne incognita

Plant Disease ◽  
2020 ◽  
Author(s):  
Nan Yin ◽  
Rui Liu ◽  
JianLong zhao ◽  
Raja Asad Ali Khan ◽  
Yan Li ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Bacillus Cereus ◽  
Organic Compounds ◽  
Meloidogyne Incognita ◽  
Solid Phase ◽  
Biological Control Agent ◽  
Nematicidal Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Root Knot Nematode ◽  
Volatile Organic

Bacillus cereus strain Bc-cm103 shows nematicidal activity and therefore has been used as a biological control agent to control the root-knot nematode Meloidogyne incognita. However, it remains unknown whether volatile organic compounds (VOCs) produced by B. cereus strain Bc-cm103 are effective in biocontrol against M. incognita. Therefore, in this study, we investigated the activity of Bc-cm103 VOCs against M. incognita. The B. cereus strain Bc-cm103 significantly repelled the second-stage juveniles (J2s) of M. incognita. In vitro evaluation of VOCs produced by the fermentation of Bc-cm103 in a three-compartment Petri dish revealed the mortality rates of M. incognita J2s as 90.8% at 24 h and 97.2% at 48 h. Additionally, evaluation of the ability of Bc-cm103 VOCs to suppress M. incognita infection in a double-layered pot test showed that root galls on cucumber roots decreased by 46.1%. Furthermore, 21 VOCs were identified from strain Bc-cm103 by solid-phase micro-extraction gas chromatography-mass spectrometry (SPME-GC-MS), including alkanes, alkenes, esters and sulfides. Among them, dimethyl disulfide (30.63%) and S-methyl ester butanethioic acid (30.29%) were reported to have strong nematicidal activity. Together, these results suggest that B. cereus strain Bc-cm103 exhibits fumigation activity against M. incognita.

scholarly journals Pathogenicity and Volatile Nematicidal Metabolites from Duddingtonia flagrans against Meloidogyne incognita

2021 ◽  
Vol 9 (11) ◽  
pp. 2268
Author(s):  
Xiaoyu Mei ◽  
Xin Wang ◽  
Guohong Li
Keyword(s):  
Meloidogyne Incognita ◽  
Plant Pathogens ◽  
Plant Root ◽  
Nematicidal Activity ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Egg Mass ◽  
Duddingtonia Flagrans ◽  
Egg Hatching ◽  
Root Knot Nematodes

Plant parasitic nematodes, especially parasitic root-knot nematodes, are one of the most destructive plant pathogens worldwide. The control of plant root-knot nematodes is extremely challenging. Duddingtonia flagrans is a type of nematode-trapping fungi (NTF), which produces three-dimensional adhesive networks to trap nematodes. In this study, the pathogenicity and volatile organic compounds (VOCs) of the NTF D. flagrans against the plant root-knot nematode, Meloidogyne incognita, were investigated. The predatory process of D. flagrans trapping M. incognita was observed using scanning electron microscopy. Gas chromatography-mass spectrometry analysis of the VOCs from D. flagrans led to the identification of 52 metabolites, of which 11 main compounds were tested individually for their activity against M. incognita. Three compounds, cyclohexanamine, cyclohexanone, and cyclohexanol, were toxic to M. incognita. Furthermore, these three VOCs inhibited egg hatching of M. incognita. Cyclohexanamine showed the highest nematicidal activity, which can cause 97.93% mortality of M. incognita at 8.71 µM within 12 h. The number of hatched juveniles per egg mass after 3 days was just 8.44 when treated with 26.14 µM cyclohexanamine. This study is the first to demonstrate the nematicidal activity of VOCs produced by D. flagrans against M. incognita, which indicates that D. flagrans has the potential to biocontrol plant root-knot nematodes.

Nematicidal activity of verrucarin A and roridin A isolated from Myrothecium verrucaria against Meloidogyne incognita

2018 ◽  
Vol 148 ◽  
pp. 133-143 ◽  
Author(s):  
Loan Thi Thanh Nguyen ◽  
Ja Yeong Jang ◽  
Tae Yoon Kim ◽  
Nan Hee Yu ◽  
Ae Ran Park ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Nematicidal Activity ◽  
Myrothecium Verrucaria

scholarly journals Effects of the Endophytic Bacteria Bacillus cereus BCM2 on Tomato Root Exudates and Meloidogyne incognita Infection

Plant Disease ◽  
2019 ◽  
Vol 103 (7) ◽  
pp. 1551-1558 ◽  
Author(s):  
Xia Li ◽  
Hai-Jing Hu ◽  
Jing-Yu Li ◽  
Cong Wang ◽  
Shuang-Lin Chen ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Root Exudates ◽  
Meloidogyne Incognita ◽  
Endophytic Bacteria ◽  
Root Exudate ◽  
Tomato Root ◽  
Second Stage ◽  
Tomato Roots ◽  
Meloidogyne Spp ◽  
Root Tissues

Root-knot nematodes (Meloidogyne spp.) cause serious crop losses worldwide. The colonization of tomato roots by endophytic bacteria Bacillus cereus BCM2 can greatly reduce Meloidogyne incognita damage, and tomato roots carrying BCM2 were repellent to M. incognita second-stage juveniles (J2). Here, the effects of BCM2 colonization on the composition of tomato root exudates was evaluated and potential mechanisms for BCM2-mediated M. incognita control explored using a linked twin-pot assay and GC-MS. On water agar plates, J2 preferentially avoided filter paper treated with tomato root exudates (organic phase only) from plants inoculated with BCM2, visiting these 67.1% less than controls. In a linked twin-pot assay, BCM2 treatment resulted in a 42.0% reduction in the number of nematodes in the soil, a 43.3% reduction in the number of galls and a 47.7% decrease in the density of M. incognita in root tissues. Analysis of root exudate composition revealed that BCM2 inoculation increased the number of components in exudates. Among these, 2,4-di-tert-butylphenol, 3,3-dimethyloctane, and n-tridecane secretions markedly increased. In repellency trials on water agar plates, J2 avoided 2,4-di-tert-butylphenol, n-tridecane, and 3,3-dimethyloctane at concentrations of 4 mmol/liter. In a linked twin-pot assay, inoculation with 2,4-di-tert-butylphenol or 3,3-dimethyloctane reduced the number of nematodes in the soil (by 54.9 and 70.6%, respectively), the number of galls (by 53.7 and 52.4%), and the number of M. incognita in root tissues (by 67.5 and 36.3%). BCM2 colonization in tomato roots affected the composition of root exudates, increasing the secretion of substances that appear to be repellent, thus decreasing M. incognita J2 infection of roots.

scholarly journals Nematicidal Constituents from the Essential Oil ofChenopodium AmbrosioidesAerial Parts

2011 ◽  
Vol 8 (s1) ◽  
pp. S143-S148 ◽  
Author(s):  
Chuan Qi Bai ◽  
Zhi Long Liu ◽  
Qi Zhi Liu
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oil ◽  
Meloidogyne Incognita ◽  
Nematicidal Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Chenopodium Ambrosioides ◽  
Chinese Medicinal Herb ◽  
Aerial Parts

Essential oil of Chinese medicinal herb,Chenopodium ambrosioidesaerial parts was found to possess nematicidal activity against the root-knot nematodes,Meloidogyne incognita. The essential oil ofC. ambrosioideswas obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 27 components of the essential oil were identified. The principal compounds inC. ambrosioidesessential oil were (Z)-ascaridole (27.27%),ρ-cymene (19.05%), isoascaridole (14.75%),α-pinene (6.33%) andα-terpinene (5.12%). Bioactivity-guided chromatographic separation of the essential oil on repeated silica gel columns led to isolate three volatile components ((Z)-ascaridole,ρ-cymene and isoascaridole) from the essential oil. The essential oil and (Z)-ascaridole exhibited strong nematicidal activity againstM. incognitawith LC50values of 49.55 μg/mL and 32.79 μg/mL, respectively.ρ-Cymene and isoascaridole also possessed nematicidal activity againstM. incognitawith LC50values of 435.89 μg/mL and 1323.51 μg/mL, respectively but weaker than the crude essential oil.

scholarly journals Glucosinolate content and nematicidal activity of Brazilian wild mustard tissues against Meloidogyne incognita in tomato

2010 ◽  
Vol 341 (1-2) ◽  
pp. 155-164 ◽  
Author(s):  
Rosângela D. L. Oliveira ◽  
Onkar D. Dhingra ◽  
André O. Lima ◽  
Gulab N. Jham ◽  
Mark A. Berhow ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Nematicidal Activity ◽  
Glucosinolate Content ◽  
Wild Mustard

Nematicidal activity of volatile organic compounds emitted by Brassica juncea, Azadirachta indica, Canavalia ensiformis, Mucuna pruriens and Cajanus cajan against Meloidogyne incognita

2014 ◽  
Vol 80 ◽  
pp. 34-43 ◽  
Author(s):  
Aline Ferreira Barros ◽  
Vicente Paulo Campos ◽  
Júlio Carlos Pereira da Silva ◽  
Márcio Pozzobon Pedroso ◽  
Flávio Henrique Vasconcelos Medeiros ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Brassica Juncea ◽  
Azadirachta Indica ◽  
Meloidogyne Incognita ◽  
Cajanus Cajan ◽  
Nematicidal Activity ◽  
Mucuna Pruriens ◽  
Canavalia Ensiformis ◽  
Volatile Organic
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