New insight into the mode of action of vancomycin dimers in bacterial cell wall synthesis

MedChemComm ◽  
2011 ◽  
Vol 2 (4) ◽  
pp. 278 ◽  
Author(s):  
Osamu Yoshida ◽  
Jun Nakamura ◽  
Hidenori Yamashiro ◽  
Kenji Miura ◽  
Sayaka Hayashi ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacterial Cell ◽  
Mode Of Action ◽  
Bacterial Cell Wall ◽  
Cell Wall Synthesis ◽  
Insight Into

scholarly journals Dramatic performance ofClostridium thermocellumexplained by its wide range of cellulase modalities

2016 ◽  
Vol 2 (2) ◽  
pp. e1501254 ◽  
Author(s):  
Qi Xu ◽  
Michael G. Resch ◽  
Kara Podkaminer ◽  
Shihui Yang ◽  
John O. Baker ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacterial Cell ◽  
Mode Of Action ◽  
Protein Complexes ◽  
Cellulose Degradation ◽  
Bacterial Cell Wall ◽  
Cellular Functions ◽  
Gene Deletions ◽  
Wide Range ◽  
Biomass Particles

Clostridium thermocellumis the most efficient microorganism for solubilizing lignocellulosic biomass known to date. Its high cellulose digestion capability is attributed to efficient cellulases consisting of both a free-enzyme system and a tethered cellulosomal system wherein carbohydrate active enzymes (CAZymes) are organized by primary and secondary scaffoldin proteins to generate large protein complexes attached to the bacterial cell wall. This study demonstrates thatC. thermocellumalso uses a type of cellulosomal system not bound to the bacterial cell wall, called the “cell-free” cellulosomal system. The cell-free cellulosome complex can be seen as a “long range cellulosome” because it can diffuse away from the cell and degrade polysaccharide substrates remotely from the bacterial cell. The contribution of these two types of cellulosomal systems inC. thermocellumwas elucidated by characterization of mutants with different combinations of scaffoldin gene deletions. The primary scaffoldin, CipA, was found to play the most important role in cellulose degradation byC. thermocellum, whereas the secondary scaffoldins have less important roles. Additionally, the distinct and efficient mode of action of theC. thermocellumexoproteome, wherein the cellulosomes splay or divide biomass particles, changes when either the primary or secondary scaffolds are removed, showing that the intact wild-type cellulosomal system is necessary for this essential mode of action. This new transcriptional and proteomic evidence shows that a functional primary scaffoldin plays a more important role compared to secondary scaffoldins in the proper regulation of CAZyme genes, cellodextrin transport, and other cellular functions.

scholarly journals Molecular architecture of the PBP2–MreC core bacterial cell wall synthesis complex

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Carlos Contreras-Martel ◽  
Alexandre Martins ◽  
Chantal Ecobichon ◽  
Daniel Maragno Trindade ◽  
Pierre-Jean Matteï ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacterial Cell ◽  
Bacterial Cell Wall ◽  
Molecular Architecture ◽  
Cell Wall Synthesis

scholarly journals A FOLIG ACID LINKED SYSTEM IN BACTERIAL CELL WALL SYNTHESIS?

1971 ◽  
Vol 24 (10) ◽  
pp. 713-714 ◽  
Author(s):  
EUGENE L. DULANEY ◽  
LESLEE M. MARX
Keyword(s):  
Cell Wall ◽  
Bacterial Cell ◽  
Bacterial Cell Wall ◽  
Cell Wall Synthesis

scholarly journals CsiA is a bacterial cell wall synthesis inhibitor contributing to DNA translocation through the cell envelope

2009 ◽  
Vol 72 (3) ◽  
pp. 779-794 ◽  
Author(s):  
Régis Stentz ◽  
Udo Wegmann ◽  
Mary Parker ◽  
Roy Bongaerts ◽  
Laurie Lesaint ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacterial Cell ◽  
Cell Envelope ◽  
Bacterial Cell Wall ◽  
Cell Wall Synthesis ◽  
Dna Translocation ◽  
Synthesis Inhibitor ◽  
Cell Wall Synthesis Inhibitor

scholarly journals Beta-Lactam Antibiotics Induce a Lethal Malfunctioning of the Bacterial Cell Wall Synthesis Machinery

Cell ◽  
2014 ◽  
Vol 159 (6) ◽  
pp. 1300-1311 ◽  
Author(s):  
Hongbaek Cho ◽  
Tsuyoshi Uehara ◽  
Thomas G. Bernhardt
Keyword(s):  
Cell Wall ◽  
Bacterial Cell ◽  
Bacterial Cell Wall ◽  
Cell Wall Synthesis ◽  
Beta Lactam ◽  
Beta Lactam Antibiotics
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