scholarly journals Heterologous production of the lantibiotic Ala(0)actagardine in Escherichia coli

2012 ◽  
Vol 48 (89) ◽  
pp. 10966 ◽  
Author(s):  
Yanxiang Shi ◽  
Alejandro Bueno ◽  
Wilfred A. van der Donk
Keyword(s):  
Escherichia Coli ◽  
Heterologous Production

Heterologous production of a sesquiterpene in Escherichia coli

2015 ◽  
Vol 37 (1se) ◽  
Author(s):  
Ly Thi Bich Thuy ◽  
Nguyen Duc Bach ◽  
Frank Hannemann ◽  
Rita Bernhardt
Keyword(s):  
Escherichia Coli ◽  
Heterologous Production

An aqueous two-phase system to pre-purify a heterologously produced siderophore

TECHNOLOGY ◽  
2016 ◽  
Vol 04 (03) ◽  
pp. 135-138
Author(s):  
Mahmoud Kamal Ahmadi ◽  
Samar Fawaz ◽  
Blaine A. Pfeifer
Keyword(s):  
Escherichia Coli ◽  
Natural Products ◽  
Cell Growth ◽  
Complex Nature ◽  
Phase System ◽  
Heterologous Production ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Purification System ◽  
Surrogate Host

Natural products span broad activities and applications; however, their access and production are often limited by native cellular sources. As a result, the heterologous production of a siderophore termed yersiniabactin (Ybt) was completed using the surrogate host Escherichia coli. Post-production and purification steps are complicated by the complex nature of most media used for cell growth, prompting the development in this work of an aqueous two-phase pre-purification system capable of rapidly and simply enhancing the concentration of the target Ybt compound.

Heterologous production of streptokinase as a secretary form in Streptomyces lividans and nonsecretary form in Escherichia coli

2010 ◽  
Vol 20 (1) ◽  
pp. 132-137 ◽  
Author(s):  
Mi-ran Kim ◽  
Yong-Hoon Choeng ◽  
Won-Jae Chi ◽  
Dae-Kyung Kang ◽  
Soon Kwang Hong
Keyword(s):  
Escherichia Coli ◽  
Streptomyces Lividans ◽  
Heterologous Production

scholarly journals Heterologous Production of 6-Deoxyerythronolide B in Escherichia coli through the Wood Werkman Cycle

Metabolites ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 228
Author(s):  
R. Axayacatl Gonzalez-Garcia ◽  
Lars K. Nielsen ◽  
Esteban Marcellin
Keyword(s):  
Escherichia Coli ◽  
Natural Products ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Structural Diversity ◽  
Heterologous Production ◽  
E Coli ◽  
Anticancer Properties ◽  
Defined Media ◽  
Extender Unit

Polyketides are a remarkable class of natural products with diverse functional and structural diversity. The class includes many medicinally important molecules with antiviral, antimicrobial, antifungal and anticancer properties. Native bacterial, fungal and plant hosts are often difficult to cultivate and coax into producing the desired product. As a result, Escherichia coli has been used for the heterologous production of polyketides, with the production of 6-deoxyerythronolide B (6-dEB) being the first example. Current strategies for production in E. coli require feeding of exogenous propionate as a source for the precursors propionyl-CoA and S-methylmalonyl-CoA. Here, we show that heterologous polyketide production is possible from glucose as the sole carbon source. The heterologous expression of eight genes from the Wood-Werkman cycle found in Propionibacteria, in combination with expression of the 6-dEB synthases DEBS1, DEBS2 and DEBS3 resulted in 6-dEB formation from glucose as the sole carbon source. Our results show that the Wood-Werkman cycle provides the required propionyl-CoA and the extender unit S-methylmalonyl-CoA to produce up to 0.81 mg/L of 6-dEB in a chemically defined media.

Heterologous production of 3-hydroxyvalerate in engineered Escherichia coli

2020 ◽  
Vol 61 ◽  
pp. 141-151 ◽  
Author(s):  
Dragan Miscevic ◽  
Kajan Srirangan ◽  
Teshager Kefale ◽  
Shane Kilpatrick ◽  
Duane A. Chung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heterologous Production ◽  
Engineered Escherichia Coli

The multigene families of actinoporins (part II): Strategies for heterologous production in Escherichia coli

Toxicon ◽  
2016 ◽  
Vol 118 ◽  
pp. 64-81 ◽  
Author(s):  
A. Valle ◽  
Y.P. Hervis ◽  
L.B.P. Socas ◽  
L. Canet ◽  
M. Faheem ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multigene Families ◽  
Heterologous Production

Heterologous production of the lipopeptide biosurfactant serrawettin W1 in Escherichia coli

2014 ◽  
Vol 181 ◽  
pp. 27-30 ◽  
Author(s):  
Stephan Thies ◽  
Beatrix Santiago-Schübel ◽  
Filip Kovačić ◽  
Frank Rosenau ◽  
Rudolf Hausmann ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heterologous Production ◽  
Lipopeptide Biosurfactant
Sign in / Sign up

Export Citation Format

Share Document