Peptide-catalyzed, bioinspired silicification for single-cell encapsulation in the imidazole-buffered system

2015 ◽  
Vol 51 (25) ◽  
pp. 5523-5525 ◽  
Author(s):  
Ji Hun Park ◽  
Insung S. Choi ◽  
Sung Ho Yang
Keyword(s):  
Single Cell ◽  
Cell Encapsulation ◽  
Yeast Cells ◽  
Mild Conditions ◽  
System P ◽  
Glass Sponges ◽  
Silica Encapsulation ◽  
Single Cell Encapsulation

Cytoprotective silica encapsulation of individual yeast cells is achieved by a peptide-catalyzed silicification under mild conditions, inspired by glass sponges.

High-throughput deterministic single-cell encapsulation and droplet pairing, fusion, and shrinkage in a single microfluidic device

2013 ◽  
Vol 35 (2-3) ◽  
pp. 385-392 ◽  
Author(s):  
Rogier M. Schoeman ◽  
Evelien W.M. Kemna ◽  
Floor Wolbers ◽  
Albert van den Berg
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
High Throughput ◽  
Cell Encapsulation ◽  
Single Cell Encapsulation

SINGLE CELL ENCAPSULATION IMPROVES STEM CELL RETENTION AND CELL-MEDIATED CARDIAC REPAIR

2014 ◽  
Vol 30 (10) ◽  
pp. S58 ◽  
Author(s):  
A.E. Mayfield ◽  
E.L. Tilokee ◽  
N. Latham ◽  
F. Rubens ◽  
D.W. Courtman ◽  
...  
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Cell Encapsulation ◽  
Cardiac Repair ◽  
Cell Retention ◽  
Single Cell Encapsulation

Micropillar array embedded system for single cell encapsulation in hydrogel

2015 ◽  
Vol 1724 ◽  
Author(s):  
Kyun Joo Park ◽  
Kyoung G. Lee ◽  
Seunghwan Seok ◽  
Bong Gill Choi ◽  
Seok Jae Lee ◽  
...  
Keyword(s):  
Embedded System ◽  
Single Cell ◽  
Single Cells ◽  
Cell Encapsulation ◽  
Polyethylene Glycol Diacrylate ◽  
Glycol Diacrylate ◽  
Practical Applications ◽  
Cell Clusters ◽  
Single Cell Encapsulation ◽  
Micropillar Array

ABSTRACTA cylindrical-shaped micropillar array embedded microfluidic device was proposed to enhance the dispersion of cell clusters and the efficiency of single cell encapsulation in hydrogel. Different sizes of micropillar arrays act as a sieve to break Escherichia coli (E. coli) aggregates into single cells in polyethylene glycol diacrylate (PEGDA) solution. We applied the external force for the continuous breakup of cell clusters, resulting in the production of more than 70% of single cells into individual hydrogel particles. This proposed strategy and device will be a useful platform to utilize genetically modified microorganisms in practical applications.

scholarly journals Deterministic trapping, encapsulation and retrieval of single-cells

Lab on a Chip ◽  
2017 ◽  
Vol 17 (13) ◽  
pp. 2186-2192 ◽  
Author(s):  
M. Sauzade ◽  
E. Brouzes
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Cell Encapsulation ◽  
Single Cell Encapsulation ◽  
Passive Strategy

Passive strategy for efficient true single-cell encapsulation.

scholarly journals Single Cell Encapsulation via Pickering Emulsion for Biopesticide Applications

ACS Omega ◽  
2018 ◽  
Vol 3 (10) ◽  
pp. 14294-14301 ◽  
Author(s):  
Noga Yaakov ◽  
Karthik Ananth Mani ◽  
Reut Felfbaum ◽  
Magen Lahat ◽  
Noam Da Costa ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Encapsulation ◽  
Pickering Emulsion ◽  
Single Cell Encapsulation

scholarly journals A Cytoprotective and Degradable Metal-Polyphenol Nanoshell for Single-Cell Encapsulation

2014 ◽  
pp. n/a-n/a ◽  
Author(s):  
Ji Hun Park ◽  
Kyunghwan Kim ◽  
Juno Lee ◽  
Ji Yu Choi ◽  
Daewha Hong ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Encapsulation ◽  
Single Cell Encapsulation

scholarly journals Metabolomic Profiling and Mechanotransduction of Single Chondrocytes Encapsulated in Alginate Microgels

2020 ◽  
Author(s):  
Jacob P. Fredrikson ◽  
Priyanka Brahmachary ◽  
Ebru Erdoğan ◽  
Zach Archambault ◽  
Ronald K. June ◽  
...  
Keyword(s):  
Single Cell ◽  
Dynamic Compression ◽  
Cartilage Tissue Engineering ◽  
Cell Encapsulation ◽  
Cartilage Tissue ◽  
Human Articular Cartilage ◽  
Mechanical Loads ◽  
Single Cell Encapsulation

AbstractHuman articular cartilage is comprised of two main components, the extracellular matrix (ECM) and the pericellular matrix (PCM). The PCM helps to protect chondrocytes in the cartilage from mechanical loads, but in patients with osteoarthritis, the PCM is weakened resulting in increased chondrocyte stress. As chondrocytes are responsible for cartilage synthesis and maintenance, it is important to understand how mechanical loads affect cellular responses of chondrocytes. Many studies have examined the chondrocyte response to in vitro mechanical loading by embedding in stiff agarose. However, these experiments are mostly performed in the absence of PCM which may obscure important responses to mechanotransduction. Here, we demonstrate that drop-based microfluidics allows culture of single chondrocytes in alginate microgels for cell-directed PCM synthesis that closely mimics the in vivo microenvironment. Chondrocytes form PCM over 10 days in these single cell microenvironments. Single cell microgels and monolayer controls were encapsulated in high stiffness agarose to mimic the cartilage PCM. After physiological dynamic compression in a custom-built bioreactor, microgels exhibited distinct metabolomic profiles from both uncompressed and monolayer controls. These results demonstrate the potential of single cell encapsulation in alginate microgels to advance cartilage tissue engineering and basic chondrocyte mechanobiology.

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