High affinity of p-sulfonatothiacalix[4]arene with phenanthroline-diium in aqueous solution

RSC Advances ◽  
2015 ◽  
Vol 5 (4) ◽  
pp. 2640-2646 ◽  
Author(s):  
Kui Wang ◽  
En-Cui Yang ◽  
Xiao-Jun Zhao ◽  
Yu Liu
Keyword(s):  
Aqueous Solution ◽  
Molecular Binding ◽  
Binding Behavior ◽  
High Affinity ◽  
Order Of Magnitude ◽  
Sulfonated Calixarenes

The molecular binding behavior of sulfonated calixarenes with phenanthroline-diium guests were systemically investigated. p-Sulfonatothiacalix[4]arene shows a high affinity with phenanthroline-diium guests in the order of magnitude of 105 M−1.

Molecular binding behavior of water-soluble calix[4]arenes with asymmetric 4,4′-bipyridinium guests in aqueous solution: regioselective recognition or not?

2016 ◽  
Vol 14 (46) ◽  
pp. 10804-10811 ◽  
Author(s):  
Kui Wang ◽  
Jian-Hua Cui ◽  
Si-Yang Xing ◽  
Hong-Xi Dou
Keyword(s):  
Aqueous Solution ◽  
Water Soluble ◽  
Molecular Binding ◽  
Binding Behavior

A unique regioselective recognition of N-methyl-N′-(naphthalen-2-ylmethyl)-4,4′-bipyridinium bromide iodide using a p-sulfonatocalix[4]arene was found.

scholarly journals Re-evaluation of the interaction of malonyl-CoA with the rat liver mitochondrial carnitine palmitoyltransferase system by using purified outer membranes

1990 ◽  
Vol 267 (1) ◽  
pp. 85-90 ◽  
Author(s):  
M P Kolodziej ◽  
V A Zammit
Keyword(s):  
Rat Liver ◽  
Specific Activity ◽  
Liver Mitochondria ◽  
Subsequent Treatment ◽  
Carnitine Palmitoyltransferase ◽  
Rat Liver Mitochondria ◽  
Outer Membranes ◽  
High Affinity ◽  
Malonyl Coa ◽  
Order Of Magnitude

1. The interaction of malonyl-CoA with the outer carnitine palmitoyltransferase (CPT) system of rat liver mitochondria was re-evaluated by using preparations of highly purified outer membranes, in the light of observations that other subcellular structures that normally contaminate crude mitochondrial preparations also contain malonyl-CoA-sensitive CPT activity. 2. In outer-membrane preparations, which were purified about 200-fold with respect to the inner-membrane-matrix fraction, malonyl-CoA binding was largely accounted for by a single high-affinity component (KD = 0.03 microM), in contrast with the dual site (low- and high-affinity) previously found with intact mitochondria. 3. There was no evidence that the decreased sensitivity of CPT to malonyl-CoA inhibition observed in outer membranes obtained from 48 h-starved rats (compared with those from fed animals) was due to a decreased ratio of malonyl-CoA binding to CPT catalytic moieties. Thus CPT specific activity and maximal high-affinity [14C]malonyl-CoA binding (expressed per mg of protein) were increased 2.2- and 2.0-fold respectively in outer membranes from 48 h-starved rats. 4. Palmitoyl-CoA at a concentration that was saturating for CPT activity (5 microM) decreased the affinity of malonyl-CoA binding by an order of magnitude, but did not alter the maximal binding of [14C]malonyl-CoA. 5. Preincubation of membranes with either tetradecylglycidyl-CoA or 2-bromopalmitoyl-CoA plus carnitine resulted in marked (greater than 80%) inhibition of high-affinity binding, concurrently with greater than 95% inhibition of CPT activity. These treatments also unmasked an effect of subsequent treatment with palmitoyl-CoA to increase low-affinity [14C]malonyl-CoA binding. 6. These data are discussed in relation to the possible mechanism of interaction between the malonyl-CoA-binding site and the active site of the enzyme.

Molecular binding behavior of bipyridium derivatives by water-soluble carboxylato-biphen[3]arene

2015 ◽  
Vol 51 (30) ◽  
pp. 6621-6624 ◽  
Author(s):  
Junwei Ma ◽  
Hongmei Deng ◽  
Suxiang Ma ◽  
Jian Li ◽  
Xueshun Jia ◽  
...  
Keyword(s):  
Water Soluble ◽  
Molecular Binding ◽  
Binding Behavior

Complexation of paraquat derivatives and bis(N-mono-substituted bipyridine) cations by a negatively charged carboxylato-biphen[3]arene in water is described.

Radioimmunoassay for 4-hydroxyoestrone in human urine

1981 ◽  
Vol 97 (2) ◽  
pp. 251-257 ◽  
Author(s):  
Günter Emons ◽  
Claudia Mente ◽  
Rudolf Knuppen ◽  
Peter Ball
Keyword(s):  
Human Urine ◽  
Gas Chromatography Mass Spectrometry ◽  
Clear Cut ◽  
High Affinity ◽  
Assay Procedure ◽  
Female Children ◽  
Bovine Serum Albumin Conjugate ◽  
Order Of Magnitude ◽  
Excretion Rates

Abstract. Under the protection of ascorbic acid a 4-hydroxyoestrone-bovine serum albumin conjugate was prepared containing intact 4-hydroxyoestrone as determined by gas chromatography-mass spectrometry. Using this antigen, antibodies with high affinity and specificity for 4-hydroxyoestrone were raised in rabbits. An assay procedure for the determination of 4-hydroxyoestrone in human urine and the assessment of its reliability are described. The following urinary excretion rates were found: male children 0.29 μg/24 h, female children 0.35 μg/24 h, men (20–40 years) 1.6 μg/24 h, men (>50 years) 1.8 μg/24 h, women, follic. 2.0 μg/24 h, pre-ov. 5.3 μg/24 h, luteal 2.4 μg/24 h, women, pregnant, first trim. 30.0 μg/24 h, second trim. 64.0 μg/24 h, third trim. 48.0 μg/24 h, women, post-men. 1.5 μg/24 h. Thus the amounts of 4-hydroxyoestrone excreted in human urine are about 1/3 to 1/10 of those of 2-hydroxyoestrone. During the menstrual cycle the excretion rates of 4-hydroxyoestrone are in the same order of magnitude as those of oestradiol and show a clear-cut pre-ovulatory peak.

scholarly journals Thermodynamic properties and cloud droplet activation of a series of oxo-acids

2010 ◽  
Vol 10 (13) ◽  
pp. 5873-5890 ◽  
Author(s):  
M. Frosch ◽  
A. A. Zardini ◽  
S. M. Platt ◽  
L. Müller ◽  
M.-C. Reinnig ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Thermodynamic Properties ◽  
Vapor Pressure ◽  
Cloud Condensation Nuclei ◽  
Cloud Droplet ◽  
Dicarboxylic Acids ◽  
Atmospheric Particulate Matter ◽  
Flow Tube ◽  
Order Of Magnitude ◽  
Ccn Activity

Abstract. We have investigated the thermodynamic properties of four aliphatic oxo-dicarboyxlic acids identified or thought to be present in atmospheric particulate matter: oxosuccinic acid, 2-oxoglutaric acid, 3-oxoglutaric acid, and 4-oxopimelic acid. The compounds were characterized in terms of their cloud condensation nuclei (CCN) activity, vapor pressure, density, and tendency to decarboxylate in aqueous solution. We deployed a variety of experimental techniques and instruments: a CCN counter, a Tandem Differential Mobililty Analyzer (TDMA) coupled with a laminar flow-tube, and liquid chromatography/mass spectrometry (LC/MS). The presence of the oxo functional group in the α-position causes the vapor pressure of the compounds to diminish by an order of magnitude with respect to the parent dicarboxylic acid, while the CCN activity is similar or increased. Dicarboxylic acids with an oxo-group in the β-position decarboxylate in aqueous solution. We studied the effects of this process on our measurements and findings.

Molecular binding behavior of a bispyridinium-containing bis(β-cyclodextrin) and its corresponding [2]rotaxane towards bile salts

2014 ◽  
Vol 12 (16) ◽  
pp. 2559 ◽  
Author(s):  
Ying-Ming Zhang ◽  
Ze Wang ◽  
Yong Chen ◽  
Hong-Zhong Chen ◽  
Fei Ding ◽  
...  
Keyword(s):  
Bile Salts ◽  
Molecular Binding ◽  
Binding Behavior

Multiplexed Detection of Nanoscale Microcantilever Deflections for High-Throughput Biomolecular Analysis

2001 ◽  
Author(s):  
Chris Dames ◽  
Guanghua Wu ◽  
Henry Lin ◽  
Aran Majumdar
Keyword(s):  
High Throughput ◽  
Surface Stress ◽  
Detection Method ◽  
Initial Deflection ◽  
Fabrication Technique ◽  
Multiplexed Detection ◽  
Molecular Binding ◽  
Parallel Detection ◽  
Order Of Magnitude ◽  
Biomolecular Analysis

Abstract We present the first steps towards a device for the parallel detection of multiple biomolecules through the binding-induced deflection of microcantilevers. In this study the thermal bimorph effect is used to model the changes in surface stress that are caused by molecular binding. A bank of five cantilevers (each 600 μm long, 645 nm thick) is actuated thermally over a range of 3 μm of tip deflection. The cantilevers are illuminated with a laser and the resulting pattern of reflected spots is used to validate a new CCD-based detection method that tracks the deflections of multiple cantilevers simultaneously. A stress-balancing fabrication technique was also implemented to reduce the initial deflection by an nearly order of magnitude.

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