A fluorescent G-quadruplex probe for the assay of base excision repair enzyme activity

2015 ◽  
Vol 51 (72) ◽  
pp. 13744-13747 ◽  
Author(s):  
Chang Yeol Lee ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Enzyme Activity ◽  
Base Excision Repair ◽  
Fluorescence Enhancement ◽  
Excision Repair ◽  
High Sensitivity ◽  
Repair Enzyme ◽  
Quadruplex Structure ◽  
G Quadruplex ◽  
Base Excision ◽  
Novel Strategy

A G-quadruplex probe incorporating 2-AP is utilized to develop a novel strategy to accurately determine UDG activity. The excision reaction promoted by UDG is designed to trigger the formation of G-quadruplex structure with significant fluorescence enhancement of 2-AP within the probe. By employing this strategy, UDG activity can be reliably determined with high sensitivity and specificity.

Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe

2013 ◽  
Vol 49 (50) ◽  
pp. 5630 ◽  
Author(s):  
Ka-Ho Leung ◽  
Hong-Zhang He ◽  
Victor Pui-Yan Ma ◽  
Hai-Jing Zhong ◽  
Daniel Shiu-Hin Chan ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Base Excision Repair ◽  
Excision Repair ◽  
Repair Enzyme ◽  
G Quadruplex ◽  
Base Excision

DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity

2015 ◽  
Vol 51 (69) ◽  
pp. 13373-13376 ◽  
Author(s):  
Zhen Wang ◽  
Yong Li ◽  
Lijun Li ◽  
Daiqi Li ◽  
Yan Huang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Graphene Oxide ◽  
Base Excision Repair ◽  
Fluorescent Protein ◽  
Excision Repair ◽  
Label Free ◽  
Repair Enzyme ◽  
Quenching Effect ◽  
Base Excision ◽  
Green Fluorescent

The interaction between supercharged green fluorescent protein (ScGFP) and graphene oxide (GO) as well as the resulting quenching effect of GO on ScGFP were investigated.

A highly sensitive strategy for base excision repair enzyme activity detection based on graphene oxide mediated fluorescence quenching and hybridization chain reaction

The Analyst ◽  
2016 ◽  
Vol 141 (1) ◽  
pp. 96-99 ◽  
Author(s):  
Qiang Xi ◽  
Jun-Jie Li ◽  
Wen-Fang Du ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang
Keyword(s):  
Enzyme Activity ◽  
Fluorescence Quenching ◽  
Base Excision Repair ◽  
Excision Repair ◽  
Hybridization Chain Reaction ◽  
Activity Detection ◽  
Chain Reaction ◽  
Repair Enzyme ◽  
Highly Sensitive ◽  
Base Excision

We report a highly sensitive strategy for UDG activity detection by combining HCR amplification and a GO-based fluorescence quenching platform.

scholarly journals Effect of DNA Glycosylases OGG1 and Neil1 on Oxidized G-Rich Motif in the KRAS Promoter

2021 ◽  
Vol 22 (3) ◽  
pp. 1137
Author(s):  
Annalisa Ferino ◽  
Luigi E. Xodo
Keyword(s):  
Oxidative Stress ◽  
Base Excision Repair ◽  
Excision Repair ◽  
Start Site ◽  
Dna Glycosylases ◽  
Repair Pathway ◽  
Transcription Start ◽  
G Quadruplex ◽  
Base Excision ◽  
Regulatory Functions

The promoter of the Kirsten ras (KRAS) proto-oncogene contains, upstream of the transcription start site, a quadruplex-forming motif called 32R with regulatory functions. As guanine under oxidative stress can be oxidized to 8-oxoguanine (8OG), we investigated the capacity of glycosylases 8-oxoguanine glycosylase (OGG1) and endonuclease VIII-like 1 (Neil1) to excise 8OG from 32R, either in duplex or G-quadruplex (G4) conformation. We found that OGG1 efficiently excised 8OG from oxidized 32R in duplex but not in G4 conformation. By contrast, glycosylase Neil1 showed more activity on the G4 than the duplex conformation. We also found that the excising activity of Neil1 on folded 32R depended on G4 topology. Our data suggest that Neil1, besides being involved in base excision repair pathway (BER), could play a role on KRAS transcription.

scholarly journals Base excision repair enzyme endonuclease III suppresses mutagenesis caused by 8-hydroxy-dGTP

DNA Repair ◽  
2008 ◽  
Vol 7 (1) ◽  
pp. 88-94 ◽  
Author(s):  
Tetsuya Suzuki ◽  
Kazuo Yamamoto ◽  
Hideyoshi Harashima ◽  
Hiroyuki Kamiya
Keyword(s):  
Base Excision Repair ◽  
Excision Repair ◽  
Repair Enzyme ◽  
Endonuclease Iii ◽  
Base Excision
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