Differently ordered carbonaceous structures synthesized by bubbled Ar or He plasmas inside methylene blue solutions with contrasting Escherichia coli growth inhibition effects

RSC Advances ◽  
2015 ◽  
Vol 5 (119) ◽  
pp. 98325-98334 ◽  
Author(s):  
D. Zaharie-Butucel ◽  
J. Papp ◽  
C. Leordean ◽  
S. D. Anghel
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Growth Inhibition ◽  
E Coli ◽  
Structure Synthesis ◽  
Coli Growth

A straightforward carbonaceous structure synthesis from methylene blue aided by Ar/He plasmas with different ordering and E. coli inhibition effects.

Characteristics of d-Tryptophan as an Antibacterial Agent: Effect of Sodium Chloride Concentration and Temperature on Escherichia coli Growth Inhibition

2017 ◽  
Vol 81 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Kaito Kan ◽  
Jian Chen ◽  
Shuso Kawamura ◽  
Shigenobu Koseki
Keyword(s):  
Escherichia Coli ◽  
Logistic Regression ◽  
Sodium Chloride ◽  
Growth Inhibition ◽  
Bacterial Growth ◽  
Logistic Regression Model ◽  
Antimicrobial Effect ◽  
Inhibitory Effect ◽  
E Coli ◽  
Coli Growth

ABSTRACT We previously reported that d-tryptophan exhibits adverse effects on bacterial physiology under osmotic stress. However, the mechanism by which d-tryptophan acts as an inhibitor and/or incompatible solute for bacterial growth has not yet been investigated in detail. In this study, we aimed to determine how osmotic pressure and temperature affect the antimicrobial effect of d-tryptophan. Even at the same level of osmotic pressure, d-tryptophan in conjunction with sodium chloride (NaCl) had a stronger inhibitory effect on the growth of Escherichia coli than that obtained by incubation with potassium chloride (KCl) and sucrose. Because d-tryptophan with NaCl showed the strongest inhibitory effect, we determined the optimum concentration combination of d-tryptophan and NaCl. The growth inhibition boundary conditions as a function of d-tryptophan and NaCl concentrations were determined by a logistic regression model. We found that the minimum level of NaCl for E. coli growth inhibition was 2.5% (w/v) together with 40 mM d-tryptophan. Moreover, the higher the NaCl concentration, the lower the concentration of d-tryptophan that was needed to inhibit bacterial growth. The logistic regression model that we developed enabled us to predict the concentrations required to inhibit bacterial growth. Furthermore, we examined the effect of incubation temperatures ranging from 15 to 46°C on the antimicrobial effect of d-tryptophan. The higher the reaction temperature, the more rapid the decrease of viable E. coli that was observed. This trend is likely attributable to activation of physiological metabolism under the optimum growth temperature. Together, our findings should make a significant contribution to the development of a novel bacterial growth control strategy using d-tryptophan.

Growth Inhibition of Escherichia coli O157:H7 and Listeria monocytogenes by Carvacrol and Eugenol Encapsulated in Surfactant Micelles

2005 ◽  
Vol 68 (12) ◽  
pp. 2559-2566 ◽  
Author(s):  
SYLVIA GAYSINSKY ◽  
P. MICHAEL DAVIDSON ◽  
BARRY D. BRUCE ◽  
JOCHEN WEISS
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Listeria Monocytogenes ◽  
Essential Oil ◽  
Growth Inhibition ◽  
Surfactant Concentration ◽  
Escherichia Coli O157 ◽  
Surfactant Micelles ◽  
E Coli ◽  
Oil Components

Growth inhibition of four strains of Escherichia coli O157:H7 (H1730, F4546, 932, and E0019) and Listeria monocytogenes (Scott A, 101, 108, and 310) by essential oil components (carvacrol and eugenol) solubilized in nonionic surfactant micelles (Surfynol 465 and 485W) was investigated. Concentrations of encapsulated essential oil components ranged from 0.02 to 1.25% depending on compound, surfactant type, and surfactant concentration (0.5 to 5%). Eugenol encapsulated in Surfynol 485W micelles was most efficient in inhibiting growth of the pathogens; 1% Surfynol 485W and 0.15% eugenol was sufficient to inhibit growth of all strains of E. coli O157:H7 and three of four strains of L. monocytogenes (Scott A, 310, and 108). The fourth strain, L. monocytogenes 101, was inhibited by 2.5% Surfynol and 0.225% eugenol. One percent Surfynol 485W in combination with 0.025% carvacrol was effective in inhibiting three of four strains of E. coli O157:H7. Strain H1730 was the most resistant strain, requiring 0.3% carvacrol and 5% surfactant for complete inhibition. Growth inhibition of L. monocytogenes by combinations of carvacrol and Surfynol 465 ranged between 0.15 and 0.35% and 1 and 3.75%, respectively. Generally, the antimicrobial activity of Surfynol 465 in combination with eugenol was higher than that for the combination with carvacrol. The potent activity was attributed to increased solubility of essential oil components in the aqueous phase due to the presence of surfactants and improved interactions of antimicrobials with microorganisms.

scholarly journals Removal of Methylene blue, Escherichia coli and Pseudomonus aeruginosa by Adsorption Process of Activated Carbon Produced from Moringa oleifera Bark

2021 ◽  
Author(s):  
Md. Shahin Azad ◽  
Syaza Azhari ◽  
Mohd Sukri Hassan
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Activated Carbon ◽  
Moringa Oleifera ◽  
Adsorption Process ◽  
Diffusion Mechanism ◽  
Bacterial Load ◽  
Batch Adsorption ◽  
Maximum Adsorption Capacity ◽  
E Coli

The utilization of biopolymer derived from Moringa oleifera bark using ZnCl2 and H2SO4 as activating agents for eliminating Methylene blue, Escherichia coli and Pseudomonas aeruginosa from producing wastewater. In this study, Methylene blue and both bacteria were effectively adsorbed by activated carbon with lowest dosage. The activated carbon was prepared from natural-by product of Moringa oleifera bark by pyrolysis in a furnace at 700°C for 1 h. The characteristics of activated carbon have been determined using Scanning Electron Microscopy (SEM), Brunauer-Emmett-Teller (BET), pHzpc (zero point charge), and FTIR spectroscopy. The obtained result were closely fitted with Freundlich isotherm model and adsorption kinetics follow the pseudo-second order model with the highest value of correlation coefficient (R2~1). Adsorption quantity was dose dependent and bacteria were maximum adsorbed using 10 mg of activated carbon as well as 25mg for methylene blue. The maximum adsorption capacity showed within 1 hour. The bacterial load was reduced by 98% for E. coli, 96% for P. aeruginosa as well as methylene blue reduced 94.2% from aqueous solution using batch adsorption methods. Adsorption process controlled by film diffusion mechanism. These result proposed that the activated carbon of Moringa oleifera can be used as a good adsorbent for the removal of Methylene blue, E. coli and P. aeruginosa.

Comparison of the β-Glucuronidase Assay and the Conventional Method for Identification of Escherichia coli on Eosin-Methylene Blue Agar

1997 ◽  
Vol 60 (1) ◽  
pp. 6-9 ◽  
Author(s):  
SHIU W. HUANG ◽  
CHUNG H. CHANG ◽  
TUNG F. TAI ◽  
TSUNG C. CHANG
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Conventional Method ◽  
Meat Products ◽  
Mixed Cultures ◽  
Effective Alternative ◽  
Methyl Red ◽  
Raw Meat ◽  
E Coli ◽  
Glucuronidase Assay

The purpose of this study was to compare the IMViC (indole, methyl red, Voges-Proskauer and citrate utilization) tests with the β-glucuronidase (GUD) assay for the identification of suspect Escherichia coli on Levine's eosin-methylene blue (EMB) agar. After testing 258 suspect E. coli colonies from raw meat and meat products, 163 and 44 were found to be E. coli and non-E. coli, respectively, by both methods. Nine isolates were IMViC positive (i.e., + + − − or − + − −) but GUD negative; among these isolates, six were confirmed to be E. coli by API 20E (bioMérieux, Marcy-I'Etoile, France) with the remaining three being non-E. coli. There were 42 isolates that were IMViC negative but GUD positive; among these isolates, seven were pure E. coli cultures, 33 were mixed cultures containing E. coli, and the remaining two were Proteus spp. The sensitivities for the identification of E. coli on EMB were 80.9% (169/209) and 97.1% (203/209), respectively, by the IMViC tests and GUD assay; whereas the specificities were 93.9% (46/49) and 95.9% (47/49), respectively, by the IMViC tests and GUD assay. It is proposed that the GUD assay can be an effective alternative to the conventional IMViC tests for the identification of suspect E. coli on EMB.

Polyoxovanadate inhibition of Escherichia coli growth shows a reverse correlation with Ca2+-ATPase inhibition

2019 ◽  
Vol 43 (45) ◽  
pp. 17577-17587 ◽  
Author(s):  
Dorinda Marques-da-Silva ◽  
Gil Fraqueza ◽  
Ricardo Lagoa ◽  
Anjana Anandan Vannathan ◽  
Sib Sankar Mal ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Reverse Correlation ◽  
E Coli ◽  
Atpase Inhibition ◽  
Coli Growth

Polyoxovanadates were recently found to be the most active among a series of polyoxometalates against bacteria. In this study, a reverse correlation was found between the Ca2+-ATPase IC50 and the E. Coli GI50 values.

scholarly journals Uji Daya Hambat Ekstrak Metanol Beberapa Jenis Porifera Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 129
Author(s):  
Megawati S. Saroinsong ◽  
Febby E. F. Kandou ◽  
Adelfia Papu ◽  
Marina F. O. Singkoh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
E Coli ◽  
Activity Test ◽  
And Staphylococcus Aureus ◽  
Paper Disc ◽  
Coli Growth

Penelitian ini bertujuan untuk menguji daya hambat dari ekstrak metanol beberapa jenis porifera terhadap pertumbuhan bakteri E. coli dan S. aureus. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer, yaitu dilakukan dengan mengukur zona hambat di sekeliling cakram kertas. Ekstrak Haliclona sp dapat menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan hasil pengukuran diameter zona hambat 13.50 mm, 20.67 mm dan 27.33 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat 10.08 mm, 12.83 mm dan 14.17 mm. Daya hambat ekstrak Agelas sp terhadap S. aureus menunjukkan diameter zona hambat 8.33 mm dan hanya menunjukkan pada konsentrasi ekstrak 90%, sementara daya hambat ekstrak Agelas sp pada konsentrasi 30%, 60% dan 90% terhadap E. coli menunjukkan diameter zona hambat sebesar 7.67 mm, 10.17 mm dan 14.17 mm. Daya hambat Spheciospongia sp terhadap S. aureus dan E. coli hanya terlihat pada konsentrasi ekstrak sebesar 90% dengan diameter zona hambat adalah 8.42 mm dan 8.75 mm. Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa ekstrak Haliclona sp memiliki potensi aktivitas antibakteri yang dapat digunakan sebagai bahan dasar pembuatan obat antibiotik.This research aimed to test the inhibition capabilities of methanol extract from several kinds of Porifera on Escherichia coli and Staphylococcus aureus growth. The antibacterial activity test using the Kirby-Bauer method, which delivered by measuring the inhibition zone around paper disc. The extract of Haliclona sp can inhibit the S. aureus growth at 30%, 60% and 90% of extracts concentration with the measurement of inhibition zone diameters are 13.50 mm, 20.67 mm and 27.33 mm; also inhibit the E. coli growth with inhibition zone diameters are 10.08 mm, 12.83 mm and 14.17 mm. The inhibition capability of Agelas sp extract on S. aureus shows that the inhibition zone diameters is 8.83 mm and only appear at 90% of extracts concentration, meanwhile the inhibition capability of Agelas sp extract at concentration 30%, 60% and 90% on E. coli shows diameters of inhibition zone are 7.67 mm, 10.17 mm and 14.17 mm. The inhibition capability of Spheciospongia sp on S. aureus and E. coli only occurred at 90% of extracts concentration with inhibition zone diameters 8.42 mm and 8.75 mm. Based on the results, it can be assumed that extracts of Haliclona sp has a potential antibacterial activity that can be used as a basic ingredients for antibiotic medicine.

scholarly journals Isolasi dan identifikasi Escherichia coli dari Sumber Air Minum Kandang Broiler serta Uji Aktivitas Antibakteri Lidah Buaya

2020 ◽  
Vol 10 (2) ◽  
pp. 106
Author(s):  
Dian Meididewi Nuraini ◽  
Morsid Andityas ◽  
Adi Paramarta ◽  
Nur Rohman Najib ◽  
Agustina Dwi Wijayanti
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Drinking Water ◽  
Inhibitory Activity ◽  
Aloe Vera ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Inhibition Test ◽  
Aloe Barbadensis ◽  
E Coli

Abstract Colibacillosis is one of the most problematic issues in the boiler industry. However, the antibiotic overuse has induced Escherichia coli resistance so that other alternative to reduce colibacillosis is needed. One of the alternatives is using aloe vera (Aloe barbadensis Miller), which has been widely used as an antibacterial agent. This study aims to isolate and identify E. coli from the broiler drinking water source and test the aloe vera antibacterial activity against it. Escherichia coli were isolated from well in three broiler farms in Moyudan District, Sleman, Yogyakarta that previously had colibacillosis. Escherichia coli were isolated using eosin methylene blue (EMB) agar and the metallic sheen colony was tested to confirm the biochemist reaction. The pure isolate of E. coli was used in the aloe vera inhibition test using Muller Hinton agar (MHA) by a Well Diffusion method. Aloe vera was processed using aquades and ethanol 70%. The aquades infusion was diluted into 12.5%, 25%, 50%, 75%, and 100% and the extract ethanol 70% was diluted into 10%, 12.5, %, 25%, 40%, and 50%. The bacterial identification showed that one of three samples contained E. coli which was then used for inhibition test. The result showed no inhibition zone in the aquades infusion while ethanol extract showed an inhibition zone in concentration 25%, 40%, and 50% of aloe vera extract with a diameter 19.5 mm, 24 mm, and 25 mm. It can be concluded that aloe vera ethanol extract has inhibitory activity against E. coli in poultry drinking water with a minimum concentration of 25%.  Keywords: Aloe vera; Broiler drinking water; Escherichia coli; Inhibitory activity   Abstrak  Colibacilosis masih menjadi permasalahan dalam industri broiler. Penggunaan antibiotik berlebihan telah menyebabkan resistensi sehingga perlu alternatif lain. Salah satu alternatif adalah menggunakan bahan alami seperti adalah lidah buaya (Aloe barbadensis Miller) yang memilliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi E. coli dari sumber air minum di kandang broiler serta menguji aktivitas inhibisi lidah buaya terhadap bakteri tersebut. Air yang digunakan sebagai sumber E. coli berasal dari sumur di tiga peternakan broiler di Kecamatan Moyudan, Sleman, Yogyakarta yang memiliki riwayat infeksi colibacilosis. Bakteri E. coli diisolasi menggunakan media eosin methylene blue (EMB) dan diuji sifat biokimia untuk mengkonfirmasi sifat bakteri E. coli. Isolat murni E. coli digunakan pada uji daya hambat bakteri dengan metode difusi sumuran menggunakan media Muller Hinton Agar (MHA). Lidah buaya diproses menggunakan aquades dan ethanol 70%. Infusa aquades diencerkan menjadi konsentrasi 12,5%, 25%, 50%, 75%, dan 100% dan ekstrak ethanol 70% diencerkan menjadi 10%, 12., %, 25%, 40%, dan 50%.  Hasil isolasi menunjukan bahwa satu sumber air dari sumur di Desa Kolowenang mengandung E. coli yang kemudian digunakan pada pengujian daya hambat. Hasil pengujian menunjukan tidak ada daya hambat yang terbentuk pada infusa aquades sedangkan ekstrak etanol lidah buaya 25%, 40%, dan 50% menunjukan adanya zona hambat sebesar 19,5 mm, 24 mm, dan 25 mm berturut-turut. Ekstrak etanol lidah buaya pada penelitian ini memiliki kemampuan menghambat pertumbuhan E. coli yang bersumber dari air minum broiler dengan konsentrasi terendah 25%. Kata kunci: Air minum broiler; Escherichia coli; Lidah buaya; Daya hambat bakteri

Inhibition of Escherichia coli by Bifidobacteria

1993 ◽  
Vol 56 (8) ◽  
pp. 713-715 ◽  
Author(s):  
SALAM A. IBRAHIM ◽  
ANATOLY BEZKOROVAINY
Keyword(s):  
Escherichia Coli ◽  
Acid Mixture ◽  
Ph Effects ◽  
Fermentation Products ◽  
E Coli ◽  
Antimicrobial Substances ◽  
Neutral Ph ◽  
Lactic Acids ◽  
Coli Growth

The ability of five ATCC bifidobacterial species to produce antimicrobial substances was investigated by testing the effects of spent bifidobacterial broths on the growth Escherichia coli in the thioglycollate medium. Such broths were most inhibitory if their pH was not readjusted to neutrality. When that was done, the inhibition ranged from 30 to 43%. Such inhibition of E. coli growth could be duplicated by a 3:2 aceticlactic acid mixture adjusted to neutral pH. It was concluded that no antibacterial substances other than acetic and lactic acids were produced by bifidobacterial strains used, and that the effects of these ubiquitous fermentation products, as well as pH effects, be taken into consideration before the existence of other antimicrobial factors is proposed.

scholarly journals Homocysteine editing and growth inhibition in Escherichia coli

Microbiology ◽  
2009 ◽  
Vol 155 (6) ◽  
pp. 1858-1865 ◽  
Author(s):  
Marta Sikora ◽  
Hieronim Jakubowski
Keyword(s):  
Escherichia Coli ◽  
Energy Dissipation ◽  
Growth Inhibition ◽  
Bacterial Growth ◽  
Growth Rates ◽  
Isoleucine Leucine ◽  
Trna Synthetases ◽  
E Coli

In Escherichia coli homocysteine (Hcy) is metabolically converted to the thioester Hcy-thiolactone in ATP-consuming reactions catalysed by methionyl-, isoleucyl- and leucyl-tRNA synthetases. Here we show that growth inhibition caused by supplementation of E. coli cultures with Hcy is accompanied by greatly increased accumulation of Hcy-thiolactone. Energy dissipation for Hcy editing increases 100-fold in the presence of exogenous Hcy and reaches one mole of ATP unproductively dissipated for Hcy-thiolactone synthesis per each mole of ATP that is consumed for methionine activation. Inhibiting Hcy-thiolactone synthesis with isoleucine, leucine or methionine accelerates bacterial growth in Hcy-supplemented cultures. Growth rates in Hcy-inhibited cultures are inversely related to the accumulation of Hcy-thiolactone. We also show that the levels of protein N-linked Hcy modestly increase in E. coli cells in Hcy-supplemented cultures. The results suggest that Hcy editing restrains bacterial growth.

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