Droplet-based magnetic bead immunoassay using microchannel-connected multiwell plates (μCHAMPs) for the detection of amyloid beta oligomers

Lab on a Chip ◽  
2016 ◽  
Vol 16 (12) ◽  
pp. 2245-2253 ◽  
Author(s):  
Min Cheol Park ◽  
Moojong Kim ◽  
Gun Taek Lim ◽  
Sung Min Kang ◽  
Seong Soo A An ◽  
...  
Keyword(s):  
Amyloid Beta ◽  
Magnetic Bead ◽  
Multiwell Plates ◽  
Bead Immunoassay

Automated operation of droplet-based magnetic bead immunoassay in the μCHAMPs.

scholarly journals Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion

2021 ◽  
Author(s):  
Marcelo S. Conzentino ◽  
Tatielle P. C. Santos ◽  
Khaled A. Selim ◽  
Berenike Wagner ◽  
Janette T. Alford ◽  
...  
Keyword(s):  
High Throughput ◽  
Magnetic Beads ◽  
Low Cost ◽  
Magnetic Bead ◽  
Minimal Amount ◽  
High Throughput Analysis ◽  
Major Drawback ◽  
Analytical Time ◽  
Magnetic Extraction ◽  
Bead Immunoassay

ABSTRACTA technique allowing high throughput, fast and low-cost quantitative analysis of human IgG antibodies reacting to SARS-CoV-2 antigens will be required to understand the levels of protecting antibodies in the population raised in response to infections and/or to immunization. We described previously a fast, simple, and inexpensive Ni2+ magnetic bead immunoassay which allowed detection of human antibodies reacting against the SARS-CoV-2 nucleocapsid protein using a minimal amount of serum or blood. A major drawback of the previously described system was that it only processed 12 samples simultaneously. Here we describe a manually operating inexpensive 96 well plate magnetic extraction / homogenization process which allows high throughput analysis delivering results of 96 samples in chromogenic format in 12 minutes or in fluorescent ultrafast format which takes only 7 minutes. We also show that His tag antigen purification can be performed on the fly while loading antigens to the Ni2+ magnetic beads in a process which takes only 12 min reducing the pre analytical time and cost. Finally, we show that the magnetic bead immunoassay is antigen flexible and can be performed using either Nucleocapsid, Spike or Spike RBD. The method performed with low inter and intra assay variability using different antigens and detection modes and was able to deliver >99.5% specificity and >95% sensitivity for a cohort of 203 pre pandemic and 63 COVID-19 positive samples.

scholarly journals Zearalenone Contamination in Corn, Corn Products, and Swine Feed in China in 2016–2018 as Assessed by Magnetic Bead Immunoassay

Toxins ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 451 ◽  
Author(s):  
Li ◽  
Yang ◽  
Mao ◽  
Hong ◽  
Du
Keyword(s):  
Limit Of Detection ◽  
Accurate Determination ◽  
Magnetic Bead ◽  
Maximal Inhibition ◽  
Detection Range ◽  
Inhibition Concentration ◽  
Corn Products ◽  
Swine Feed ◽  
Bead Immunoassay

In total, 405 samples of corn, corn products, and swine feed from China in 2016–2018 were surveyed for zearalenone (ZEN) contamination using a magnetic bead immunoassay-coupled biotin–streptavidin system (BAS-MBI). The developed BAS-MBI had a limit of detection (LOD) of 0.098 ng mL−1, with half-maximal inhibition concentration (IC50) of 0.71 ng mL−1 in working buffer, and an LOD of 0.98 ng g−1; the detection range was from 0.98 to 51.6 ng g−1 in authentic agricultural samples. The BAS-MBI has been demonstrated to be a powerful method for the rapid, sensitive, specific, and accurate determination of ZEN. The ZEN positivity rate reached the highest level of 40.6% in 133 samples in 2016; ZEN levels ranged from 1.8 to 1100.0 ng g−1, with an average level of 217.9 ng g−1. In 2017, the ZEN positivity rate was the lowest at 24.5% in 143 samples; ZEN levels ranged from 1.1 to 722.6 ng g−1, with an average of 166.7 ng g−1. In 2018, the ZEN positivity rate was 31.8% in 129 samples; ZEN levels ranged from 1.3 to 947.8 ng g−1, with an average of 157.0 ng g−1. About 20% of ZEN-positive samples exceeded maximum limit levels. An alternative method of ZEN detection and a valuable reference for ZEN contamination in corn and its related products in China are provided. This survey suggests the need for prevention of serious ZEN contamination, along with management for food safety and human health.

scholarly journals A magnetic bead immunoassay to detect human IgG reactive to SARS-CoV-2 Spike S1 RBD produced in Escherichia coli

2021 ◽  
Author(s):  
Marcelo dos Santos Conzentino ◽  
Ana C Goncalves ◽  
NIgella M Paula ◽  
Fabiane G Rego ◽  
Dalila Zanette ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Receptor Binding ◽  
High Throughput ◽  
Low Cost ◽  
Magnetic Bead ◽  
Binding Domain ◽  
Receptor Binding Domain ◽  
Operating Characteristics ◽  
Human Igg ◽  
Bead Immunoassay

Immunological assays to detect SARS-CoV-2 Spike receptor binding domain antigen seroconversion in humans are important tools to monitor the levels of protecting antibodies in the population in response to infection and/or immunization. Here we describe a simple, low cost and high throughput Ni2+ magnetic bead immunoassay to detect human IgG reactive to Spike S1 RBD Receptor Binding Domain produced in Escherichia coli. A 6xHis tagged Spike S1 RBD was expressed in E. coli and purified by affinity chromatography. The protein was mobilized on the surface of Ni2+ magnetic beads and used to investigate the presence of reactive IgG in the serum obtained from pre-pandemic and COVID-19 confirmed cases. The method was validated with a cohort of 290 samples and an area under the receiver operating characteristics curve of 0.94 was obtained. The method operated with>82% sensitivity at 98% specificity and was also able to track human IgG raised in response to vaccination with Comirnaty with 85% sensitivity. The IgG signal obtained with the described method was well correlated with the signal obtained when pre fusion Spike produced in HEK cell lines were used as antigen. This novel low-cost and high throughput immunoassay may act as an important tool to investigate protecting IgG antibodies against SARS-CoV-2 in the human population.

Proteolytic processing of the amyloid-beta protein precursor of Alzheimer's disease

2002 ◽  
Vol 38 ◽  
pp. 37-49 ◽  
Author(s):  
Janelle Nunan ◽  
David H Small
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Amyloid Beta ◽  
Alternative Pathway ◽  
Protein A ◽  
Proteolytic Processing ◽  
Gamma Secretase ◽  
Amyloid Beta Protein ◽  
Protein Precursor ◽  
Amyloid Beta Protein Precursor

The proteolytic processing of the amyloid-beta protein precursor plays a key role in the development of Alzheimer's disease. Cleavage of the amyloid-beta protein precursor may occur via two pathways, both of which involve the action of proteases called secretases. One pathway, involving beta- and gamma-secretase, liberates amyloid-beta protein, a protein associated with the neurodegeneration seen in Alzheimer's disease. The alternative pathway, involving alpha-secretase, precludes amyloid-beta protein formation. In this review, we describe the progress that has been made in identifying the secretases and their potential as therapeutic targets in the treatment or prevention of Alzheimer's disease.

Antibody Clears Amyloid-Beta From Brains of Alzheimer's Patients

2012 ◽  
Vol 40 (2) ◽  
pp. 20 ◽  
Author(s):  
MITCHEL L. ZOLER
Keyword(s):  
Amyloid Beta

CSF-concentrations of tau-protein, phospho-tau-protein (181) and amyloid-beta (1–42) in patients with stable and non-stable MCI

2009 ◽  
Vol 42 (05) ◽  
Author(s):  
E Kaiser ◽  
PA Thomann ◽  
U Seidl ◽  
J Schröder
Keyword(s):  
Amyloid Beta ◽  
Tau Protein

Mitochondrial failure precedes amyloid beta plaques deposition in APP transgenic mice

2005 ◽  
Vol 38 (05) ◽  
Author(s):  
A Eckert ◽  
I Scherping ◽  
A Bonert ◽  
S Hauptmann ◽  
F Müller-Spahn ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Amyloid Beta
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