UV-assisted synthesis of long-wavelength Si-pyronine fluorescent dyes for real-time and dynamic imaging of glutathione fluctuation in living cells

2016 ◽  
Vol 4 (28) ◽  
pp. 4826-4831 ◽  
Author(s):  
Hailiang Nie ◽  
Liang Qiao ◽  
Wen Yang ◽  
Bingpeng Guo ◽  
Fangyun Xin ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Dyes ◽  
Living Cells ◽  
Dynamic Imaging ◽  
Long Wavelength

Long-wavelength Si-pyronine fluorescent dyes are synthesized for reversible, real-time and dynamic imaging of glutathione fluctuation in living cells.

Real-time monitoring of intracellular nitric oxide using a long-wavelength-emitting probe via one-photon or two-photon excitation

2019 ◽  
Vol 7 (11) ◽  
pp. 3246-3252 ◽  
Author(s):  
Changquan Tang ◽  
Mingxue Wang ◽  
Xiaoying Shang ◽  
Xueyuan Chen ◽  
Deguang Huang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Long Wavelength ◽  
Two Photon ◽  
Turn On ◽  
Photon Excitation ◽  
Endogenous Nitric Oxide ◽  
Two Photon Excitation

A long-wavelength-emitting fluorescent turn-on probe is developed for monitoring exogenous or endogenous nitric oxide in living cells.

Biomass carbon dots derived from Wedelia trilobata for the direct detection of glutathione and their imaging application in living cells

2021 ◽  
Author(s):  
Caizhen Liang ◽  
Xiaobao Xie ◽  
Dandan Zhang ◽  
Jin Feng ◽  
Shunying Lu ◽  
...  
Keyword(s):  
Real Time ◽  
Carbon Dots ◽  
Direct Detection ◽  
Living Cells ◽  
Biomass Carbon ◽  
Real Time Monitoring ◽  
Long Wavelength ◽  
Wedelia Trilobata ◽  
Imaging Application ◽  
Wavelength Emission

Novel long-wavelength emission carbon dots were successfully synthesized from biomass Wedelia trilobata for the direct detection of GSH, which exhibited an intriguing capacity for real-time monitoring of the variation in intracellular GSH levels.

Morphogenetic machines revealed: Microscopy of living cells in the embryo of the frog, Xenopus laevis

1993 ◽  
Vol 51 ◽  
pp. 172-173
Author(s):  
Ray Keller
Keyword(s):  
Image Processing ◽  
Fluorescent Dyes ◽  
Cell Movement ◽  
Living Cells ◽  
Ease Of Use ◽  
Low Light ◽  
Amphibian Embryo ◽  
Large Populations ◽  
Light Fluorescence ◽  
Video Image Processing

The amphibian embryo offers advantages of size, availability, and ease of use with both microsurgical and molecular methods in the analysis of fundamental developmental and cell biological problems. However, conventional wisdom holds that the opacity of this embryo limits the use of methods in optical microscopy to resolve the cell motility underlying the major shape-generating processes in early development.These difficulties have been circumvented by refining and adapting several methods. First, methods of explanting and culturing tissues were developed that expose the deep, nonepithelial cells, as well as the superficial epithelial cells, to the view of the microscope. Second, low angle epi-illumination with video image processing and recording was used to follow patterns of cell movement in large populations of cells. Lastly, cells were labeled with vital, fluorescent dyes, and their behavior recorded, using low-light, fluorescence microscopy and image processing. Using these methods, the details of the cellular protrusive activity that drives the powerful convergence (narrowing)

A multicolor DNA tetrahedron nanoprobe for analyzing human telomerase in living cells

2021 ◽  
Author(s):  
Ruiyuan Zhang ◽  
Ruixue Zhang ◽  
Wei Jiang ◽  
Xiaowen Xu
Keyword(s):  
Real Time ◽  
Length Distribution ◽  
Telomerase Activity ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Dna Tetrahedron ◽  
Human Telomerase

A sequentially lighting-up multicolor DNA tetrahedron nanoprobe is constructed for imaging telomerase activity, real-time monitoring telomerase action and determining product length distribution in living cells.

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