Electrically controlled rapid release of actives encapsulated in double-emulsion droplets

Lab on a Chip ◽  
2018 ◽  
Vol 18 (7) ◽  
pp. 1121-1129 ◽  
Author(s):  
Yankai Jia ◽  
Yukun Ren ◽  
Likai Hou ◽  
Weiyu Liu ◽  
Tianyi Jiang ◽  
...  
Keyword(s):  
Electric Fields ◽  
Double Emulsion ◽  
On Demand ◽  
Emulsion Droplets ◽  
Rapid Release ◽  
Oil In Water ◽  
Double Emulsion Droplets

We utilize electric fields to trigger the on-demand release of different cargos that are encapsulated in water-in-oil-in-water (W/O/W) double-emulsion droplets.

scholarly journals Dielectrophoresis Response of Water-in-Oil-in-Water Double Emulsion Droplets with Singular or Dual Cores

Micromachines ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1121
Author(s):  
Tianyi Jiang ◽  
Yankai Jia ◽  
Haizhen Sun ◽  
Xiaokang Deng ◽  
Dewei Tang ◽  
...  
Keyword(s):  
Electric Fields ◽  
Strong Field ◽  
Microfluidic Channel ◽  
Double Emulsion ◽  
Multiple Emulsion ◽  
Field Frequency ◽  
Emulsion Droplets ◽  
Oil In Water ◽  
Wide Range ◽  
Double Emulsion Droplets

Microfluidic technologies have enabled generation of exquisite multiple emulsion droplets, which have been used in many fields, including single-cell assays, micro-sized chemical reactions, and material syntheses. Electrical controlling is an important technique for droplet manipulation in microfluidic systems, but the dielectrophoretic behaviors of multiple emulsion droplets in electrical fields are rarely studied. Here, we report on the dielectrophoresis response of double emulsion droplets in AC electric fields in microfluidic channel. A core-shell model is utilized for analyzing the polarization of droplet interfaces and the overall dielectrophoresis (DEP) force. The water-in-oil-in-water droplets, generated by glass capillary devices, experience negative DEP at low field frequency. At high frequency, however, the polarity of DEP is tunable by adjusting droplet shell thickness or core conductivity. Then, the behavior of droplets with two inner cores is investigated, where the droplets undergo rotation before being repelled or attracted by the strong field area. This work should benefit a wide range of applications that require manipulation of double emulsion droplets by electric fields.

scholarly journals A multiplexed cell-free assay to screen for antimicrobial peptides in double emulsion droplets

2021 ◽  
Author(s):  
Nicola Nuti ◽  
Philipp Rottmann ◽  
Ariane Stucki ◽  
Philipp Koch ◽  
Sven Panke ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
High Frequency ◽  
Bacterial Resistance ◽  
Double Emulsion ◽  
Microfluidic System ◽  
Promising Candidate ◽  
Emulsion Droplets ◽  
Oil In Water ◽  
Double Emulsion Droplets ◽  
Automated Methods

The global surge in bacterial resistance against traditional antibiotics triggered intensive research for novel compounds, with antimicrobial peptides (AMPs) identified as a promising candidate. Automated methods to systematically generate and screen AMPs according to their membrane preference, however, are still lacking. We introduce a novel microfluidic system for the simultaneous cell-free production and screening of AMPs for their membrane specificity. On our device, AMPs are cell-free produced within water-in-oil-in-water double emulsion droplets, generated at high frequency. Within each droplet, the peptides can interact with different classes of co-encapsulated liposomes, generating a membrane-specific fluorescent signal. The double emulsions can be incubated and observed in a hydrodynamic trapping array or analysed via flow cytometry. Our approach provides a valuable tool for the discovery and development of membrane-active antimicrobials.

scholarly journals Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry

Micromachines ◽  
2013 ◽  
Vol 4 (4) ◽  
pp. 402-413 ◽  
Author(s):  
Jing Yan ◽  
Wolfgang-Andreas Bauer ◽  
Martin Fischlechner ◽  
Florian Hollfelder ◽  
Clemens Kaminski ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Double Emulsion ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Emulsion Droplets ◽  
Oil In Water ◽  
Double Emulsion Droplets

Forming concentric double-emulsion droplets using electric fields

2009 ◽  
Vol 67 (2-3) ◽  
pp. 173-177 ◽  
Author(s):  
Z.-M. Bei ◽  
T.B. Jones ◽  
A. Tucker-Schwartz
Keyword(s):  
Electric Fields ◽  
Double Emulsion ◽  
Emulsion Droplets ◽  
Double Emulsion Droplets

scholarly journals Production of giant unilamellar vesicles and encapsulation of lyotropic nematic liquid crystals

Soft Matter ◽  
2021 ◽  
Author(s):  
Peng Bao ◽  
Daniel A. Paterson ◽  
Sally A. Peyman ◽  
J. Cliff Jones ◽  
Jonathan A. T. Sandoe ◽  
...  
Keyword(s):  
Liquid Crystals ◽  
Nematic Liquid Crystals ◽  
Double Emulsion ◽  
Lyotropic Liquid Crystals ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Emulsion Droplets ◽  
Double Emulsion Droplets

We describe a modified microfluidic method for making Giant Unilamellar Vesicles (GUVs) via water/octanol-lipid/water double emulsion droplets and encapsulation of nematic lyotropic liquid crystals (LNLCs).

A microfluidic platform for formation of double-emulsion droplets

2009 ◽  
Vol 7 (5) ◽  
pp. 709-719 ◽  
Author(s):  
Kin-Lok Lao ◽  
Jung-Hao Wang ◽  
Gwo-Bin Lee
Keyword(s):  
Double Emulsion ◽  
Microfluidic Platform ◽  
Emulsion Droplets ◽  
Double Emulsion Droplets

scholarly journals Double emulsion flow cytometry with high-throughput single droplet isolation and nucleic acid recovery

Lab on a Chip ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 2062-2074 ◽  
Author(s):  
Kara K. Brower ◽  
Catherine Carswell-Crumpton ◽  
Sandy Klemm ◽  
Bianca Cruz ◽  
Gaeun Kim ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
High Throughput ◽  
Double Emulsion ◽  
Single Droplet ◽  
Emulsion Droplets ◽  
Acid Recovery ◽  
Emulsion Flow ◽  
Double Emulsion Droplets

We have developed a novel workflow (sdDE-FACS, s̲ingle d̲roplet D̲ouble E̲mulsion FACS) that allows robust production, screening, and sorting of single double emulsion droplets with complete nucleic acid recovery.

scholarly journals Rapid formation of multicellular spheroids in double-emulsion droplets with controllable microenvironment

2013 ◽  
Vol 3 (1) ◽  
Author(s):  
Hon Fai Chan ◽  
Ying Zhang ◽  
Yi-Ping Ho ◽  
Ya-Ling Chiu ◽  
Youngmee Jung ◽  
...  
Keyword(s):  
Double Emulsion ◽  
Multicellular Spheroids ◽  
Emulsion Droplets ◽  
Rapid Formation ◽  
Double Emulsion Droplets
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