scholarly journals Rapid detection of staphylococcal enterotoxin B in milk samples based on fluorescence hybridization chain reaction amplification

RSC Advances ◽  
2018 ◽  
Vol 8 (29) ◽  
pp. 16024-16031 ◽  
Author(s):  
Yanyang Xu ◽  
Bingyang Huo ◽  
Xuan Sun ◽  
Baoan Ning ◽  
Yuan Peng ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Staphylococcal Enterotoxin ◽  
Hybridization Chain Reaction ◽  
Staphylococcal Enterotoxin B ◽  
Chain Reaction ◽  
Milk Samples ◽  
Reaction Amplification ◽  
Enterotoxin B

A new competitive aptasensor combined with HCR was developed for SEB detection.

Immunomagnetic Flow Cytometric Detection of Staphylococcal Enterotoxin B in Raw and Dry Milk

2003 ◽  
Vol 66 (7) ◽  
pp. 1222-1226 ◽  
Author(s):  
TAKAHISA MIYAMOTO ◽  
HIDEAKI KAMIKADO ◽  
HIROSHI KOBAYASHI ◽  
KEN-ICHI HONJOH ◽  
MASAYOSHI IIO
Keyword(s):  
Fluorescence Intensity ◽  
Magnetic Beads ◽  
Immunomagnetic Separation ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Igg Antibody ◽  
Milk Samples ◽  
Rabbit Igg ◽  
Dry Milk ◽  
Enterotoxin B

A rapid and sensitive method for detection of staphylococcal enterotoxin B (SEB) in raw and dry milk samples with the use of antibody-based immunomagnetic separation (IMS) in conjunction with flow cytometry (FCM) was developed. Sheep anti-SEB immunoglobulin G (IgG) was immobilized on Dynabeads M-280. The SEB initially binds to the capturing antibody, which is bound on the magnetic beads. The rabbit anti-SEB IgG binds to the captured toxin and is further labeled with a Cy5-labeled goat anti-rabbit IgG antibody. The percentage of the beads that were fluorescent was measured by FCM. FCM was carried out for 1 min, and the data obtained were expressed as histograms for particle size (forward light scatter) and histograms for fluorescence intensity. A peak corresponding to the magnetic beads was clearly distinguished from a peak derived from contaminating particles in the sample solution. In the absence of SEB, about 10% of the beads emitted fluorescence. The percentage of fluorescent beads and the fluorescence intensity increased with increasing SEB concentrations. For this IMS-FCM assay, the lower limits of detection for SEB were estimated to be 0.01 and 0.25 ng/ml for buffer and milk samples, respectively.

scholarly journals Rapid and simultaneous detection of ricin, staphylococcal enterotoxin B and saxitoxin by chemiluminescence-based microarray immunoassay

The Analyst ◽  
2014 ◽  
Vol 139 (22) ◽  
pp. 5885-5892 ◽  
Author(s):  
A. Szkola ◽  
E. M. Linares ◽  
S. Worbs ◽  
B. G. Dorner ◽  
R. Dietrich ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Microarray Immunoassay ◽  
Enterotoxin B ◽  
Idiotypic Antibody

Simultaneous detection of proteotoxins, ricin and SEB, and small toxin, STX, on a chemiluminescence-based microarray using anti-idiotypic antibody for STX.

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