A capacitive DNA sensor for sensitive detection of Escherichia coli O157:H7 in potable water based on the z3276 genetic marker: fabrication and analytical performance

The Analyst ◽  
2020 ◽  
Vol 145 (6) ◽  
pp. 2267-2278 ◽  
Author(s):  
Rehan Deshmukh ◽  
Arun Kumar Prusty ◽  
Utpal Roy ◽  
Sunil Bhand
Keyword(s):  
Escherichia Coli ◽  
Genetic Marker ◽  
Potable Water ◽  
Analytical Performance ◽  
Sensitive Detection ◽  
Escherichia Coli O157 ◽  
Dna Sensor ◽  
Label Free ◽  
Dna Sensing ◽  
Water Based

We report a label-free biosensor for the detection of Escherichia coli O157:H7 ATCC 43895 in potable water using a newly designed DNA sensing probe targeting the z3276 genetic marker.

Label-free capacitive immunosensor based on quartz crystal Au electrode for rapid and sensitive detection of Escherichia coli O157:H7

2011 ◽  
Vol 687 (1) ◽  
pp. 89-96 ◽  
Author(s):  
Dujuan Li ◽  
Yangyang Feng ◽  
Ling Zhou ◽  
Zunzhong Ye ◽  
Jianping Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quartz Crystal ◽  
Sensitive Detection ◽  
Escherichia Coli O157 ◽  
Label Free ◽  
Au Electrode

Prevalence of Escherichia coli O157 in Cattle and Swine in Central Mexico†

2004 ◽  
Vol 67 (10) ◽  
pp. 2274-2276 ◽  
Author(s):  
T. R. CALLAWAY ◽  
R. C. ANDERSON ◽  
G. TELLEZ ◽  
C. ROSARIO ◽  
G. M. NAVA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Limit Of Detection ◽  
The United States ◽  
Sensitive Detection ◽  
Central Mexico ◽  
Escherichia Coli O157 ◽  
Immunomagnetic Bead ◽  
E Coli ◽  
Detection Techniques ◽  
Swine Farms

Escherichia coli O157:H7 is a foodborne pathogenic bacterium that can reside undetected in the gastrointestinal tract of cattle because colonization by this bacterium is asymptomatic. Recent research has indicated that swine can carry and transmit this pathogen as well. The development of more advanced and sensitive detection techniques has improved the limit of detection and increased sensitivity for this important pathogen. This study was undertaken to determine the prevalence of E. coli O157 in cattle and swine in Mexico with the more sensitive detection technique of immunomagnetic bead separation. Samples (n = 60 per farm) were taken from four cattle and four swine farms (n = 240 cattle samples, n = 240 swine samples) located throughout central Mexico in October 2001. The prevalence of E. coli O157 was found to be only 1.25% on cattle farms and 2.1% on swine farms. The prevalence in cattle in this study is lower than that reported in the United States and could be related to the lower reported prevalence of E. coli O157 in humans in Mexico. However, further research is needed to verify prevalence throughout other regions of Mexico, as well as prevalence during other seasons of the year.

scholarly journals A novel fluorometric aptasensor based on carbon nanocomposite for sensitive detection of Escherichia coli O157:H7 in milk

2020 ◽  
Vol 103 (9) ◽  
pp. 7879-7889
Author(s):  
Tao Yang ◽  
Xinyan Yang ◽  
Xiaojie Guo ◽  
Shiqian Fu ◽  
Jiapeng Zheng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sensitive Detection ◽  
Escherichia Coli O157 ◽  
Carbon Nanocomposite

scholarly journals Sensitive Detection of Escherichia coli O157:H7 in Food Products by Impedimetric Immunosensors

Sensors ◽  
2018 ◽  
Vol 18 (7) ◽  
pp. 2168 ◽  
Author(s):  
Francesca Malvano ◽  
Roberto Pilloton ◽  
Donatella Albanese
Keyword(s):  
Escherichia Coli ◽  
Food Products ◽  
Sensitive Detection ◽  
Escherichia Coli O157

10-Minute Assay for Detecting Escherichia coli O157:H7 in Ground Beef Samples Using Piezoelectric-Excited Millimeter-Size Cantilever Sensors

2007 ◽  
Vol 70 (7) ◽  
pp. 1670-1677 ◽  
Author(s):  
DAVID MARALDO ◽  
RAJ MUTHARASAN
Keyword(s):  
Escherichia Coli ◽  
Sample Preparation ◽  
Ground Beef ◽  
Low Ph ◽  
Frequency Change ◽  
Escherichia Coli O157 ◽  
Label Free ◽  
Cantilever Sensors ◽  
Ph Buffer ◽  
Phosphate Buffered Saline

We detected Escherichia coli O157:H7 (EC) at approximately 10 cells per ml in spiked ground beef samples in 10 min using piezoelectric-excited millimeter-size cantilever (PEMC) sensors. The composite PEMC sensors have a sensing area of 2mm2 and are prepared by immobilizing a polyclonal antibody specific to EC on the sensing surface. Ground beef (2.5 g) was spiked with EC at 10 to 10,000 cells per ml in phosphate-buffered saline (PBS). One milliliter of supernatant was removed from the blended samples and used to perform the detection experiments. The total resonant frequency change obtained for the inoculated samples was 138 ± 9, 735 ± 23, 2,603 ± 51, and 7,184 ± 606 Hz, corresponding to EC concentrations of 10, 100, 1,000, and 10,000 cells per ml, respectively. EC was detected in the sample solution within the first 10 min. The responses of the sensor to positive, negative, and buffer controls were 36 ± 6, 27 ± 2, and 2 ± 7 Hz, respectively. Verification of EC attachment was confirmed by low-pH buffer release (PBS-HCl, pH 2.2), microscopy, and second antibody EC binding postdetection. The results indicate that PEMC sensors can reliably detect EC at less than 10 cells per ml in 10 min without sample preparation and with label-free reagents.

Label-free amperometric biosensor for Escherichia coli O157:H7 detection

2019 ◽  
Vol 495 ◽  
pp. 143548 ◽  
Author(s):  
Nidhi Dhull ◽  
Gurpreet Kaur ◽  
Prateek Jain ◽  
Priyanka Mishra ◽  
Divya Singh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amperometric Biosensor ◽  
Escherichia Coli O157 ◽  
Label Free
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