scholarly journals Identification of potential sialic acid binding proteins on cell membranes by proximity chemical labeling

2019 ◽  
Vol 10 (24) ◽  
pp. 6199-6209 ◽  
Author(s):  
Qiongyu Li ◽  
Yixuan Xie ◽  
Gege Xu ◽  
Carlito B. Lebrilla
Keyword(s):  
Sialic Acid ◽  
Protein Oxidation ◽  
Cell Membranes ◽  
Binding Proteins ◽  
Binding Protein ◽  
Chemical Labeling ◽  
Acid Binding Protein ◽  
Mapping Tool ◽  
Sialic Acid Binding ◽  
Protein Discovery

A “protein oxidation of sialic acid environments” (POSE) mapping tool is developed for sialic acid binding protein discovery.

scholarly journals A Glycal-Based Photoaffinity Probe That Enriches Sialic Acid Binding Proteins

2019 ◽  
Author(s):  
Peter Thuy-Boun ◽  
Dennis Wolan
Keyword(s):  
Sialic Acid ◽  
Binding Proteins ◽  
Binding Protein ◽  
Factor H ◽  
Protein Profiling ◽  
Complement Factor ◽  
Acid Binding Protein ◽  
Sialic Acid Binding ◽  
Kinetic Inhibition ◽  
Protein Complement

<p>To identify sialic acid binding proteins from complex proteomes, three photocrosslinking affinity-based probes were constructed using Neu5Ac (<b>5 </b>and <b>6</b>) and Neu5Ac2en (<b>7</b>) scaffolds. Kinetic inhibition assays and Western blotting revealed the Neu5Ac2en-based <b>7 </b>to be an effective probe for the labeling of a purified gut microbial sialidase (BDI_2946) and a purified human sialic acid binding protein (hCD33). Additionally, LC-MS/MS affinity-based protein profiling verified the ability of <b>7</b>to enrich a low-abundance sialic acid binding protein (complement factor H) from human serum thus validating the utility of this probe in a complex context.</p>

scholarly journals A Glycal-Based Photoaffinity Probe That Enriches Sialic Acid Binding Proteins

2019 ◽  
Author(s):  
Peter Thuy-Boun ◽  
Dennis Wolan
Keyword(s):  
Sialic Acid ◽  
Binding Proteins ◽  
Binding Protein ◽  
Factor H ◽  
Protein Profiling ◽  
Complement Factor ◽  
Acid Binding Protein ◽  
Sialic Acid Binding ◽  
Kinetic Inhibition ◽  
Protein Complement

<p>To identify sialic acid binding proteins from complex proteomes, three photocrosslinking affinity-based probes were constructed using Neu5Ac (<b>5 </b>and <b>6</b>) and Neu5Ac2en (<b>7</b>) scaffolds. Kinetic inhibition assays and Western blotting revealed the Neu5Ac2en-based <b>7 </b>to be an effective probe for the labeling of a purified gut microbial sialidase (BDI_2946) and a purified human sialic acid binding protein (hCD33). Additionally, LC-MS/MS affinity-based protein profiling verified the ability of <b>7</b>to enrich a low-abundance sialic acid binding protein (complement factor H) from human serum thus validating the utility of this probe in a complex context.</p>

Purification of sialic acid binding protein from saprophytic bacteria by hydrophobic-interaction chromatography on butyl-toyopearl and polymer-coated porous glass

1993 ◽  
Vol 7 (9) ◽  
pp. 667-670 ◽  
Author(s):  
Larisa S. Zhigis ◽  
Alexander E. Ivanov ◽  
Eugenya M. Rapoport ◽  
Emma A. Kovalenko ◽  
Ekaterina I. Getman ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Hydrophobic Interaction ◽  
Porous Glass ◽  
Binding Protein ◽  
Hydrophobic Interaction Chromatography ◽  
Acid Binding Protein ◽  
Sialic Acid Binding

scholarly journals A Sialic Acid-Binding Protein SABP1 of Toxoplasma gondii Mediates Host Cell Attachment and Invasion

2020 ◽  
Vol 222 (1) ◽  
pp. 126-135 ◽  
Author(s):  
Mengen Xing ◽  
Na Yang ◽  
Ning Jiang ◽  
Dawei Wang ◽  
Xiaoyu Sang ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Toxoplasma Gondii ◽  
Host Cell ◽  
Cell Attachment ◽  
Binding Protein ◽  
Critical Role ◽  
Uncharacterized Protein ◽  
Neuraminidase Treatment ◽  
Acid Binding Protein ◽  
Sialic Acid Binding

Abstract Many obligate intracellular apicomplexan parasites have adapted a distinct invasion mechanism involving a close interaction between the parasite ligands and the sialic acid (SA) receptor. We found that sialic acid binding protein-1 (SABP1), localized on the outer membrane of the zoonotic parasite Toxoplasma gondii, readily binds to sialic acid on the host cell surface. The binding was sensitive to neuraminidase treatment. Cells preincubated with recombinant SABP1 protein resisted parasite invasion in vitro. The parasite lost its invasion capacity and animal infectivity after the SABP1 gene was deleted, whereas complementation of the SABP1 gene restored the virulence of the knockout strain. These data establish the critical role of SABP1 in the invasion process of T. gondii. The previously uncharacterized protein, SABP1, facilitated T. gondii attachment and invasion via sialic acid receptors.

Identification of a cell cycle-dependent gene product as a sialic acid-binding protein

1989 ◽  
Vol 163 (1) ◽  
pp. 506-512 ◽  
Author(s):  
Hans-J. Gabius ◽  
Attila Bardosi ◽  
Sigrun Gabius ◽  
Klaus P. Hellmann ◽  
Michael Karas ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Sialic Acid ◽  
Gene Product ◽  
Binding Protein ◽  
Acid Binding Protein ◽  
Sialic Acid Binding ◽  
A Cell ◽  
Cycle Dependent

scholarly journals Preparation of a sialic acid-binding protein from Streptococcus mitis KS32AR.

1986 ◽  
Vol 53 (2) ◽  
pp. 359-365 ◽  
Author(s):  
P A Murray ◽  
M J Levine ◽  
M S Reddy ◽  
L A Tabak ◽  
E J Bergey
Keyword(s):  
Sialic Acid ◽  
Binding Protein ◽  
Streptococcus Mitis ◽  
Acid Binding Protein ◽  
Sialic Acid Binding

scholarly journals The Sialic Acid Binding Protein, Hsa, in Streptococcus gordonii DL1 also Mediates Intergeneric Coaggregation with Veillonella Species

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0143898 ◽  
Author(s):  
Peng Zhou ◽  
Jinman Liu ◽  
Xiaoli Li ◽  
Yukihiro Takahashi ◽  
Fengxia Qi
Keyword(s):  
Sialic Acid ◽  
Binding Protein ◽  
Streptococcus Gordonii ◽  
Acid Binding Protein ◽  
Sialic Acid Binding

scholarly journals Structural and functional characterization of NanU, a novel high-affinity sialic acid-inducible binding protein of oral and gut-dwelling Bacteroidetes species

2014 ◽  
Vol 458 (3) ◽  
pp. 499-511 ◽  
Author(s):  
Chatchawal Phansopa ◽  
Sumita Roy ◽  
John B. Rafferty ◽  
C. W. Ian Douglas ◽  
Jagroop Pandhal ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Binding Protein ◽  
Functional Characterization ◽  
Acid Binding Protein ◽  
High Affinity ◽  
Sialic Acid Binding ◽  
Polysaccharide Utilization Locus ◽  
Specific Polysaccharide ◽  
Human Dwelling

We biochemically and structurally characterize a novel inducible surface-associated sialic acid-binding protein present in human-dwelling Bacteroidetes species that represents a novel type of sialic acid-specific polysaccharide utilization locus.

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