NanoPADs and nanoFACEs: an optically transparent nanopaper-based device for biomedical applications

Lab on a Chip ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 3322-3333
Author(s):  
Binbin Ying ◽  
Siwan Park ◽  
Longyan Chen ◽  
Xianke Dong ◽  
Edmond W. K. Young ◽  
...  
Keyword(s):  
Cell Culture ◽  
Microfluidic Device ◽  
Biomedical Applications ◽  
Adherent Cell ◽  
Optically Transparent ◽  
Adherent Cell Culture

A highly transparent nanopaper-based microfluidic device for chemical/biosensing and cell culture, which is branded as nanopaper-based analytical devices (nanoPADs) and nanofibrillated adherent cell-culture platforms (nanoFACEs).

scholarly journals Microfluidic device capable of medium recirculation for non-adherent cell culture

2014 ◽  
Vol 8 (1) ◽  
pp. 016503 ◽  
Author(s):  
Angela R. Dixon ◽  
Shrinidhi Rajan ◽  
Chuan-Hsien Kuo ◽  
Tom Bersano ◽  
Rachel Wold ◽  
...  
Keyword(s):  
Cell Culture ◽  
Microfluidic Device ◽  
Adherent Cell ◽  
Adherent Cell Culture

scholarly journals Use of a non-adherent cell culture system for testing the effect of 2′,3′-dideoxyinosine againstCryptosporidium parvum

1999 ◽  
Vol 176 (1) ◽  
pp. 235-240
Author(s):  
Philippe Lawton ◽  
Carine Hejl ◽  
Marie-Elisabeth Sarciron ◽  
Roselyne Mancassola ◽  
Muriel Naciri ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Adherent Cell ◽  
Cell Culture System ◽  
Adherent Cell Culture

scholarly journals The Combined Effects of Co-Culture and Substrate Mechanics on 3D Tumor Spheroid Formation within Microgels Prepared via Flow-Focusing Microfluidic Fabrication

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 229 ◽  
Author(s):  
Dongjin Lee ◽  
Chaenyung Cha
Keyword(s):  
Mechanical Properties ◽  
Cell Culture ◽  
Microfluidic Device ◽  
3D Cell Culture ◽  
Breast Adenocarcinoma ◽  
Adherent Cell ◽  
Flow Focusing ◽  
Spheroid Formation ◽  
3D Scaffold ◽  
Tumor Spheroids

Tumor spheroids are considered a valuable three dimensional (3D) tissue model to study various aspects of tumor physiology for biomedical applications such as tissue engineering and drug screening as well as basic scientific endeavors, as several cell types can efficiently form spheroids by themselves in both suspension and adherent cell cultures. However, it is more desirable to utilize a 3D scaffold with tunable properties to create more physiologically relevant tumor spheroids as well as optimize their formation. In this study, bioactive spherical microgels supporting 3D cell culture are fabricated by a flow-focusing microfluidic device. Uniform-sized aqueous droplets of gel precursor solution dispersed with cells generated by the microfluidic device are photocrosslinked to fabricate cell-laden microgels. Their mechanical properties are controlled by the concentration of gel-forming polymer. Using breast adenocarcinoma cells, MCF-7, the effect of mechanical properties of microgels on their proliferation and the eventual spheroid formation was explored. Furthermore, the tumor cells are co-cultured with macrophages of fibroblasts, which are known to play a prominent role in tumor physiology, within the microgels to explore their role in spheroid formation. Taken together, the results from this study provide the design strategy for creating tumor spheroids utilizing mechanically-tunable microgels as 3D cell culture platform.

Laminin-adherent versus suspension-non-adherent cell culture conditions for the isolation of cancer stem cells in the DAOY medulloblastoma cell line

Tumor Biology ◽  
2016 ◽  
Vol 37 (9) ◽  
pp. 12359-12370 ◽  
Author(s):  
Javier de la Rosa ◽  
Ander Sáenz Antoñanzas ◽  
Mehdi H. Shahi ◽  
Bárbara Meléndez ◽  
Juan A. Rey ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Cancer Stem Cells ◽  
Cell Line ◽  
Culture Conditions ◽  
Adherent Cell ◽  
Medulloblastoma Cell ◽  
Medulloblastoma Cell Line ◽  
Adherent Cell Culture ◽  
Cell Culture Conditions

Development of rolled scaffold for high-density adherent cell culture

2019 ◽  
Vol 22 (1) ◽  
Author(s):  
Ashkan YekrangSafakar ◽  
Katie M. Hamel ◽  
Ali Mehrnezhad ◽  
Jangwook P. Jung ◽  
Kidong Park
Keyword(s):  
Cell Culture ◽  
High Density ◽  
Adherent Cell ◽  
Adherent Cell Culture

scholarly journals Quantification of Carbon Nanotube Doses in Adherent Cell Culture Assays Using UV-VIS-NIR Spectroscopy

Nanomaterials ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1765 ◽  
Author(s):  
Dedy Septiadi ◽  
Laura Rodriguez-Lorenzo ◽  
Sandor Balog ◽  
Miguel Spuch-Calvar ◽  
Giovanni Spiaggia ◽  
...  
Keyword(s):  
Cell Culture ◽  
Colloidal Stability ◽  
Near Infrared ◽  
Culture Media ◽  
Nir Spectroscopy ◽  
Lung Epithelial Cells ◽  
Adherent Cell ◽  
Adherent Cell Culture ◽  
Protein Rich

The overt hazard of carbon nanotubes (CNTs) is often assessed using in vitro methods, but determining a dose–response relationship is still a challenge due to the analytical difficulty of quantifying the dose delivered to cells. An approach to accurately quantify CNT doses for submerged in vitro adherent cell culture systems using UV-VIS-near-infrared (NIR) spectroscopy is provided here. Two types of multi-walled CNTs (MWCNTs), Mitsui-7 and Nanocyl, which are dispersed in protein rich cell culture media, are studied as tested materials. Post 48 h of CNT incubation, the cellular fractions are subjected to microwave-assisted acid digestion/oxidation treatment, which eliminates biological matrix interference and improves CNT colloidal stability. The retrieved oxidized CNTs are analyzed and quantified using UV-VIS-NIR spectroscopy. In vitro imaging and quantification data in the presence of human lung epithelial cells (A549) confirm that up to 85% of Mitsui-7 and 48% for Nanocyl sediment interact (either through internalization or adherence) with cells during the 48 h of incubation. This finding is further confirmed using a sedimentation approach to estimate the delivered dose by measuring the depletion profile of the CNTs.

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