scholarly journals Development of a multicolor upconversion fluorescence immunoassay for the simultaneous detection of thiamethoxam and dextran by magnetic separation

RSC Advances ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 517-524
Author(s):  
Chuanyong Li ◽  
Wanlin Sun ◽  
Lianrun Huang ◽  
Nana Sun ◽  
Xiude Hua ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Simultaneous Determination ◽  
Magnetic Separation ◽  
Simultaneous Detection ◽  
Fluorescence Immunoassay ◽  
Upconversion Fluorescence

The anti-thiamethoxam and anti-dextran monoclonal antibodies were prepared to develop a multicolor upconversion fluorescence immunoassay for the simultaneous determination of thiamethoxam (544 nm) and dextran (477 nm).

scholarly journals MnO2@Reduced Graphene Oxide Nanocomposite-Based Electrochemical Sensor for the Simultaneous Determination of Trace Cd(II), Zn(II) and Cu(II) in Water Samples

Membranes ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 517
Author(s):  
Siyamthanda Hope Mnyipika ◽  
Tshimangadzo Saddam Munonde ◽  
Philiswa Nosizo Nomngongo
Keyword(s):  
Heavy Metals ◽  
Electrochemical Sensor ◽  
Simultaneous Determination ◽  
Rapid Detection ◽  
Electrochemical Sensors ◽  
Simultaneous Detection ◽  
Cyclic Voltammograms ◽  
Current Response ◽  
Mno2 Nanoparticles

The rapid detection of trace metals is one of the most important aspect in achieving environmental monitoring and protection. Electrochemical sensors remain a key solution for rapid detection of heavy metals in environmental water matrices. This paper reports the fabrication of an electrochemical sensor obtained by the simultaneous electrodeposition of MnO2 nanoparticles and RGO nanosheets on the surface of a glassy carbon electrode. The successful electrodeposition was confirmed by the enhanced current response on the cyclic voltammograms. The XRD, HR-SEM/EDX, TEM, FTIR, and BET characterization confirmed the successful synthesis of MnO2 nanoparticles, RGO nanosheets, and MnO2@RGO nanocomposite. The electrochemical studies results revealed that MnO2@RGO@GCE nanocomposite considerably improved the current response on the detection of Zn(II), Cd(II) and Cu(II) ions in surface water. These remarkable improvements were due to the interaction between MnO2 nanomaterials and RGO nanosheets. Moreover, the modified sensor electrode portrayed high sensitivity, reproducibility, and stability on the simultaneous determination of Zn(II), Cd(II), and Cu(II) ions. The detection limits of (S/N = 3) ranged from 0.002–0.015 μg L−1 for the simultaneous detection of Zn(II), Cd(II), and Cu(II) ions. The results show that MnO2@RGO nanocomposite can be successfully used for the early detection of heavy metals with higher sensitivity in water sample analysis.

scholarly journals A dual-label time-resolved fluorescence immunoassay for the simultaneous determination of ferritin and β2-microglobulin

2017 ◽  
Vol 31 (6) ◽  
pp. e22132 ◽  
Author(s):  
Zhi Liu ◽  
Jing Huang ◽  
Rui-ming Ou ◽  
Meng-dong Yao ◽  
Yan-lin She ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Fluorescence Immunoassay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Β2 Microglobulin ◽  
Dual Label ◽  
Label Time

scholarly journals Determination of Morphine in Human Urine by the Novel Competitive Fluorescence Immunoassay

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Jie Cao ◽  
Xiao-Ying Chen ◽  
Wu-Rong Zhao
Keyword(s):  
Monoclonal Antibodies ◽  
Human Urine ◽  
Screening Tool ◽  
Fluorescein Isothiocyanate ◽  
Cross Reactivity ◽  
The Novel ◽  
Fluorescence Immunoassay ◽  
Coated Plate ◽  
Efficient Screening

A competitive fluorescence immunoassay for the identification and quantification of morphine has been developed on the basis of hapten-coated plate format. Hapten was prepared through covalent conjugating a morphine derivative with albumin bovine. In the immunoassay, the hapten was inoculated on a 96-well plate and then bound with monoclonal antibodies labeled with a signal indicating dye, fluorescein isothiocyanate (FITC). Unbound FITC-antibodies were rinsed off from the plate. The fluorescein intensity decreases in the presence of morphine molecules due to the competitively binding to antibodies against hapten. The intensity is inversely correlated with the concentration of morphine. In quantitative analysis for urine samples, we obtained a linearity range of 0.2 μg/mL∼2.5 μg/mL, along with a detection limit of c.a. 1 ng/mL. The fluorescence immunoassay shows low cross-reactivity (below 10%) to 6-acetylmorphine, 3-acetylmorphine, and heroine. The developed method produced comparable results to the standard GC-MS/MS method. In conclusion, a rapid and efficient screening tool for morphine in clinical human urine has been established.

scholarly journals Development of a method for the simultaneous detection of Cr(iii) and Cr(vi) in exhaled breath condensate samples using μLC-ICP-MS

2016 ◽  
Vol 31 (4) ◽  
pp. 924-933 ◽  
Author(s):  
Elizabeth Leese ◽  
Jackie Morton ◽  
Philip H. E. Gardiner ◽  
Vikki A. Carolan
Keyword(s):  
Simultaneous Determination ◽  
Method Development ◽  
Exhaled Breath Condensate ◽  
Simultaneous Detection ◽  
Exhaled Breath ◽  
Development Study ◽  
Icp Ms ◽  
Breath Condensate

A method development study describing the first simultaneous determination of Cr(III) and Cr(VI) in an exhaled breath condensate sample.

Simultaneous Detection of Several Fusarium Mycotoxins in Cereals, Grains, and Foodstuffs

1981 ◽  
Vol 64 (5) ◽  
pp. 1067-1073 ◽  
Author(s):  
Hisashi Kamimura ◽  
Motohiro Nishijima ◽  
Kazuo Yasuda ◽  
Kazuo Saito ◽  
Akihiro Ibe ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Simultaneous Determination ◽  
Simultaneous Detection ◽  
Fusarium Mycotoxins ◽  
Aqueous Methanol ◽  
Systematic Method ◽  
Chromatographic Procedure ◽  
Cereal Samples

Abstract A systematic method is described for the simultaneous determination of Fusarium mycotoxins (nivalenol, deoxynivalenol, fusarenon-x, diacetoxyscirpenol, neosolaniol, T-2 toxin, HT-2 toxin, butenolide, moniliformin, and zearalenone) in cereals, grains, and foodstuffs. Mycotoxins were extracted with aqueous methanol and purified by a 2-step chromatographic procedure using Amberlite XAD-4 and Florisil columns. The column eluates were concentrated and spotted on a thin layer chromatographic (TLC) plate which was then developed in CHCL3-methanol (93 + 7) and toluene-acetonemethanol (5 + 3 + 2). Each mycotoxin was quantitated by gas chromatography (GO and TLC densitometry. The minimum detectable concentrations (μg/kg) in various test materials were: nivalenol, deoxynivalenol, and fusarenon-x, 2.0; diacetoxyscirpenol, neosolaniol, T-2 toxin, and HT-2 toxin, 80; zearalenone, 10; butenolide, 30; and moniliformin, 50. Recoveries of the mycotoxins added to various cereal samples at 1.0-2.0 Mg/g were greater than 71% and averaged 85%.

scholarly journals Simultaneous Determination of α-Fetoprotein and Free β-Human Chorionic Gonadotropin by Element-Tagged Immunoassay with Detection by Inductively Coupled Plasma Mass Spectrometry

2004 ◽  
Vol 50 (7) ◽  
pp. 1214-1221 ◽  
Author(s):  
Sichun Zhang ◽  
Chao Zhang ◽  
Zhi Xing ◽  
Xinrong Zhang
Keyword(s):  
Mass Spectrometry ◽  
Monoclonal Antibodies ◽  
Simultaneous Determination ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Inductively Coupled Plasma ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

Abstract Background: An inductively coupled plasma mass spectrometry (ICP-MS)-based immunoassay has been proposed independently by Baranov et al. (Anal Chem 2002;74:1629–36) and our group, but the applicability of this method for multianalyte analysis in clinical samples has not been fully illustrated. We developed a dual-label immunoassay method for the simultaneous determination of α-fetoprotein (AFP) and free β-human chorionic gonadotropin (hCGβ) in human serum. Methods: Monoclonal antibodies immobilized on microtiter plates captured AFP and hCGβ, which were detected by use of Eu3+-labeled anti-AFP and Sm3+-labeled anti-hCGβ monoclonal antibodies. Eu3+ and Sm3+ were dissociated from the immunocomplex with HNO3 solution (10 mL/L) and delivered by peristaltic pump to the ICP mass spectrometer. Results: The measurable ranges of AFP and hCGβ were 4.6–500 and 5.0–170 μg/L, respectively, with detection limits of 1.2 and 1.7 μg/L (3 SD above mean of zero calibrator), respectively. The intraassay imprecision (CV) for AFP was 8.3%, 4.0%, and 2.7% at 16.3, 86, and 354 μg/L, respectively, and the interassay CV was 10%, 5.7%, and 3.5%. For hCGβ, the intraassay CV was 5.4%, 6.4%, and 3.1%, respectively, at 10.5, 45.2, and 105 μg/L, and the interassay CV was 7.2%, 8.0%, and 3.7%. Comparison with IRMAs for AFP and hCGβ yielded correlation coefficients (r2) of 0.97 and 0.95. Conclusions: Two proteins can be measured simultaneously by immunoassays using two rare earth elemental tags (Eu3+ and Sm3+) and ICP-MS detection. The multielement capability and the multiple potential elemental labels make ICP-MS attractive for multianalyte immunoassays. Implementation of ICP-MS-linked immunoassays may be relatively straightforward because the labeling and immunoreaction procedures have been well developed for clinical time-resolved immunofluorometric assays.

Preparation of Broadly Specific Monoclonal Antibodies for Simultaneous Determination of Fluoroquinolone Residues in Eggs

2016 ◽  
Vol 9 (12) ◽  
pp. 3520-3531 ◽  
Author(s):  
Dapeng Peng ◽  
Yulian Wang ◽  
Liang Feng ◽  
Guangcai Cao ◽  
Yanfei Tao ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Simultaneous Determination

Simultaneous determination of eight ginsenosides in Zibu Piyin Recipe using ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UPLC-MS/MS)

2015 ◽  
Vol 7 (18) ◽  
pp. 7527-7533
Author(s):  
Hui-Ying Xu ◽  
Hong Xiang ◽  
Li-Bin Zhan ◽  
Lin Zhang ◽  
Xiao-Xin Sun
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Simultaneous Detection ◽  
Ultra Performance Liquid Chromatography ◽  
Triple Quadrupole ◽  
Quadrupole Mass ◽  
Quadrupole Mass Spectrometry ◽  
Detection And Quantification

A simple, sensitive, selective, and reliable UPLC-MS/MS method was developed and validated for the simultaneous detection and quantification of eight compounds found in Zibu Piyin Recipe (ZBPYR).

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