Towards high-throughput optimization of microbial lipid production: from strain development to process monitoring

2020 ◽  
Vol 4 (12) ◽  
pp. 5958-5969
Author(s):  
Dania Awad ◽  
Samer Younes ◽  
Matthias Glemser ◽  
Franz M. Wagner ◽  
Gerhard Schenk ◽  
...  

We describe, for the first time, the use of fast neutron irradiation for the rapid metabolic optimization of an oleaginous yeast. The approach established in this study provides a progressive path towards automatable strain development efforts.

2011 ◽  
Vol 35 (5) ◽  
pp. 1906-1911 ◽  
Author(s):  
Jie Zhang ◽  
Xu Fang ◽  
Xiao-Ling Zhu ◽  
Yan Li ◽  
Hai-Peng Xu ◽  
...  

Energies ◽  
2019 ◽  
Vol 12 (6) ◽  
pp. 1053 ◽  
Author(s):  
Xiaozan Dai ◽  
Hongwei Shen ◽  
Qiang Li ◽  
Kamal Rasool ◽  
Qian Wang ◽  
...  

Dry acid pretreatment and biodetoxification (DryPB) has been considered as an advanced technology to treat lignocellulosic materials for improved downstream bioconversion. In this study, the lipid production from DryPB corn stover was investigated by the oleaginous yeast Rhodosporidium toruloides using a new process designated prehydrolysis followed by simultaneous saccharification and lipid production (PreSSLP). The results found that prehydrolysis at 50 °C and then lipid production at 30 °C improved lipid yield by more than 17.0% compared with those without a prehydrolysis step. The highest lipid yield of 0.080 g/g DryPB corn stover was achieved at a solid loading of 12.5%. The fatty acid distribution of lipid products was similar to those of conventional vegetable oils that are used for biodiesel production. Our results suggested that the integration of DryPB process and PreSSLP process can be explored as an improved technology for microbial lipid production from lignocellulosic materials.


RSC Advances ◽  
2014 ◽  
Vol 4 (66) ◽  
pp. 34944 ◽  
Author(s):  
Jintao Lin ◽  
Shuangyue Li ◽  
Mingzhong Sun ◽  
Cuili Zhang ◽  
Wenbo Yang ◽  
...  

2015 ◽  
Vol 72 ◽  
pp. 95-103 ◽  
Author(s):  
Yi-Huang Chang ◽  
Ku-Shang Chang ◽  
Ching-Fu Lee ◽  
Chuan-Liang Hsu ◽  
Cheng-Wei Huang ◽  
...  

2021 ◽  
Vol 9 (34) ◽  
pp. 11439-11447
Author(s):  
Emmanuel D. Revellame ◽  
Alex Zappi ◽  
Rafael Hernandez ◽  
Daniel Gang ◽  
William E. Holmes ◽  
...  

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