A simple high-yield procedure for isolation of human urinary kallikreins
Keyword(s):
Two human urinary kallikreins (fractions A-1 and A-2) were purified to apparently homogeneous forms. The two kallikreins were separated by affinity chromatography using Trasylol (aprotinin) covalently bound to Sepharose. The kallikreins were eluted with a pH gradient (pH 9.5-3.0). Fraction A-1 was eluted between pH 6.2 and 4.2 and fraction A-2 was eluted between pH 4.2 and 3.1. Final purification was obtained by chromatography on Sephacryl SS-200. Antibodies prepared against fraction A-2 were also reactive with fraction A-1; thus it may be possible to measure both by radioimmunoassay using the same antibody preparation.
1983 ◽
Vol 268
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2020 ◽
1978 ◽
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pp. 1198-1205
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2017 ◽
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pp. 104-111
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1976 ◽
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pp. 586-593
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1979 ◽
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pp. 171-175
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Keyword(s):
1982 ◽
Vol 126
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pp. 149-153
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