scholarly journals Decoction of heat-clearing, detoxifying and blood stasis removing relieves acute soft tissue injury via modulating miR-26b-5p/COX2 axis to inhibit inflammation

2020 ◽  
Vol 40 (12) ◽  
Author(s):  
Shunwan Jiang ◽  
Zhi Chen ◽  
Wenqiang Lai ◽  
Qingchun Mai ◽  
Dayu Chen ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Cell Line ◽  
Tissue Injury ◽  
Blood Stasis ◽  
Soft Tissue Injury ◽  
Enzyme Linked Immunosorbent Assay ◽  
Microvascular Endothelium ◽  
In Vivo Models ◽  
Tnf Α

Abstract Traditional Chinese medicine (TCM), such as Huanglian-Jie-Du-Tang, a heat-clearing and detoxifying decoction is beneficial for alleviation of inflammation-related diseases. The objective of the present study is to uncover the effect and mechanism of heat-clearing, detoxifying and blood stasis removing decoction (HDBD) on the treatment of acute soft tissue injury (STI) which is characterized with excessive inflammatory cascade at the onset. Male Sprague–Dawley (SD) rats with hammer beating served as the in vivo models of acute STI. Hematoxylin–Eosin (HE) staining was used for histopathology assessment. The levels of inflammatory factors, including prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α), interleukin (IL)-1t and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA). Human dermal microvascular endothelium cell line HMEC-1 and rat vascular endothelium cell line RAOEC were used to explore the mechanism in vitro. Luciferase gene reporter assay was applied to determine the relationship between miR-26b-5p and Cyclo-oxygenase 2 (COX2). The results showed that HDBD intervention significantly reduced the temperature difference between the healthy side and affected side of rats with hammer beating, together with the decreased levels of COX2, PGE2, TNF-α, IL-6 and IL-1β, and the increased level of miR-26b-5p. In mechanism, miR-26b-5p targeted COX2 and decreased its expression, leading to significant decreases in the levels of PGE2, TNF-α and IL-6 in RAOEC and HMEC-1 cells. In addition, miR-26b-5p inhibition impaired the effects of HDBD on the suppression of PGE2, TNF-α, IL-6 and IL-1β in vitro. In conclusion, the present study revealed that HDBD relieved acute STI via modulating miR-26b-5p/COX2 axis to inhibit inflammation.

scholarly journals Cefazolin sodium pentahydrate combined with vacuum sealing drainage in the treatment of open fracture complicated with soft tissue injury

2020 ◽  
Vol 66 (4) ◽  
pp. 430-436
Author(s):  
Wenjie Lou ◽  
Kai Zou ◽  
Zhongbin Yu ◽  
Song Qin ◽  
Kai Wang ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Open Fracture ◽  
Tissue Injury ◽  
Soft Tissue Injury ◽  
Serum Levels ◽  
Control Group ◽  
Observation Group ◽  
Tnf Α ◽  
Cefazolin Sodium ◽  
Vacuum Sealing Drainage

SUMMARY OBJECTIVE To investigate the clinical efficacy of cefazolin sodium pentahydrate combined with vacuum sealing drainage (VSD) in the treatment of open fracture complicated with soft tissue injury. METHODS Sixty-three patients with open fracture complicated with soft tissue injury were divided into observation (n = 33) and control (n = 30) groups. After surgical reduction, fixation, and repair of the fractures, the control group was treated with VSD for 10 days, and the observation group was treated with cefazolin sodium pentahydrate based on VSD for 10 days. The infection control time was recorded. After treatment, the pain of patients was evaluated. Before and after treatment, the serum levels of C-reactive protein (CRP), interleukin (IL)-6, IL-8, tumor necrosis factor α (TNF-α), cortisol, epinephrine, norepinephrine, and glucose were detected. After 6 months of treatment, the total effective rate of the treatment was evaluated. RESULTS The infection control time and Visual Analogue Scale score after treatment in the observation group were significantly lower than in the control group, respectively (P < 0.05). After the treatment, the serum levels of CRP, IL-6, IL-8, TNF-α, cortisol, epinephrine, norepinephrine, and glucose in each group were significantly lower than before the treatment (P < 0.05), and each index in observation was significantly lower than in the control group (P < 0.05). CONCLUSIONS In the treatment of open fractures complicated with soft tissue injury, cefazolin sodium pentahydrate combined with VSD can effectively reduce inflammation and stress, thus improving the treatment efficacy.

Auto seat restraint soft-tissue injury

1987 ◽  
Vol 148 (2) ◽  
pp. 458-458 ◽  
Author(s):  
DR Pennes ◽  
WA Phillips
Keyword(s):  
Soft Tissue ◽  
Tissue Injury ◽  
Soft Tissue Injury

Soft tissue injury associated with intravenous phenytoin in a neonate

1994 ◽  
Vol 83 (11) ◽  
pp. 1218-1219 ◽  
Author(s):  
N. Sharief ◽  
C. Goonasekera
Keyword(s):  
Soft Tissue ◽  
Tissue Injury ◽  
Soft Tissue Injury

Establishment of soft-tissue-injury model of high-voltage electrical burn and observation of its pathological changes

Burns ◽  
2009 ◽  
Vol 35 (8) ◽  
pp. 1158-1164 ◽  
Author(s):  
Chai Jia-ke ◽  
Li Li-gen ◽  
Gao Quan-wen ◽  
Shen Xiao-peng ◽  
Zhang Hai-jun ◽  
...  
Keyword(s):  
Soft Tissue ◽  
High Voltage ◽  
Tissue Injury ◽  
Soft Tissue Injury ◽  
Pathological Changes ◽  
Injury Model

The Hazards of Skateboard-Riding

PEDIATRICS ◽  
1976 ◽  
Vol 57 (5) ◽  
pp. 793-793
Author(s):  
◽  
Fernando Atienza ◽  
Calvin Sia
Keyword(s):  
Emergency Department ◽  
Soft Tissue ◽  
Bone Fractures ◽  
Tissue Injury ◽  
Skull Fracture ◽  
Soft Tissue Injury ◽  
Soft Tissue Injuries ◽  
Major Surgery ◽  
Cerebral Concussion ◽  
Tissue Injuries

Skateboard-riding has become increasingly popular among Hawaii's children. The thrill of the ride and the challenge of keeping one's balance and working intricate maneuvers while speeding down a hill captures the fancy of many of our young. This sport, however, has produced an alarmingly high toll of injury and illness. Pediatricians and emergency departments of our major hospitals have seen and taken care of large numbers of patients (aged between 3 years and 35 years, but with a distribution overwhelmingly pediatric) with significant injuries which include cerebral concussion, fractures, soft tissue injuries of varying degrees of severity and complications, and injury to internal organs. During a three-month period at the Kauikeolani Children's Hospital, July to August 1975, there were 16 patients admitted with the following: seven cerebral concussions, one skull fracture, five assorted bone fractures, one soft tissue injury and infection, one retroperitoneal hemorrhage, and one instance of major surgery for removal of the spleen. During the months of August and September 1975 the Emergency Department of Straub Clinic reported the following skateboard injuries: 14 fractures, 14 soft tissue injuries, 5 lacerations, and 2 cerebral concussions. Of the 35 patients seen, three were admitted—one with an open fracture, one with cerebral concussion, and one with a skull fracture. During a four-week period (two weeks in June and July and two weeks in August and September) at the Emergency Department of Kaiser Medical Center, 66 cases of skateboard injuries were seen with six patients requiring admission for fractures and brain concussion.

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