124 Screening for Bence Jones proteinuria using the Coomassie Brilliant Blue and Pyrogallol Red protein assay values

1997 ◽  
Vol 25 (4) ◽  
pp. S658-S658 ◽  
Author(s):  
KATHERINE M. WILLIAMS ◽  
THOMAS MARSHALL ◽  
NICOLA J. ABBOTT ◽  
JOHN WILLIAMS
Keyword(s):  
Brilliant Blue ◽  
Coomassie Brilliant Blue ◽  
Protein Assay ◽  
Pyrogallol Red ◽  
Coomassie Brilliant ◽  
Bence Jones Proteinuria

Study of Protein Adsorption and Conformational Change on DLC and Ti Surfaces Using FTIR and Coomassie Brilliant Blue Protein Assay

2007 ◽  
Vol 330-332 ◽  
pp. 893-896 ◽  
Author(s):  
Huang Yan ◽  
Xiao Ying Lü ◽  
Nan Huang
Keyword(s):  
Secondary Structure ◽  
Structure Analysis ◽  
Brilliant Blue ◽  
Material Surface ◽  
Coomassie Brilliant Blue ◽  
Protein Assay ◽  
Human Plasma Protein ◽  
Secondary Structure Analysis ◽  
Adsorbed Proteins ◽  
Coomassie Brilliant

The aim of present work was to study the interaction between human plasma protein-albumin (Alb) and immunoglobulin G (IgG) and the surfaces of two kinds of diamond-like carbon (DLC-A and DLC-B) and titanium (Ti) film. Fourier transform infrared spectroscopy (FTIR) was used to perform both quantity investigation and secondary structure analysis of above two proteins adsorbed on material surfaces. A modified Coomassie brilliant blue (CBB) protein assay was also used to study the amount of adsorbed proteins. The result of FTIR quantitative evaluation shows that the ratio of adsorbed Alb to IgG (RA/I) on three kinds of material surface has an order: DLC-A > DLC-B > Ti, which is coincide with the result from CBB protein assay. The result of secondary structure analysis shows that the conformation of Alb and IgG changes in a largest degree after adsorbed on Ti and a smallest degree on DLC-A surface. Both the results indicate that the anti-thrombogenicity of DLC-A seems to be the best and Ti is the worst.

A Mathematical Model for the Description of the Coomassie Brilliant Blue Protein Assay

1996 ◽  
Vol 233 (2) ◽  
pp. 160-168 ◽  
Author(s):  
B.A. Atherton ◽  
E.L. Cunningham ◽  
A.G. Splittgerber
Keyword(s):  
Mathematical Model ◽  
Brilliant Blue ◽  
Coomassie Brilliant Blue ◽  
Protein Assay ◽  
Coomassie Brilliant

A Comparison of Three Procedures for the Detection of Bence–Jones Proteinuria

1997 ◽  
Vol 34 (4) ◽  
pp. 371-374 ◽  
Author(s):  
W K Wong ◽  
G E Wieringa ◽  
Z Stec ◽  
J Russell ◽  
S Cooke ◽  
...  
Keyword(s):  
Reference Method ◽  
Urine Concentration ◽  
Brilliant Blue ◽  
Suitable Alternative ◽  
Coomassie Brilliant Blue ◽  
Enhanced Sensitivity ◽  
Traditional Procedure ◽  
Amido Black ◽  
Coomassie Brilliant ◽  
Bence Jones Proteinuria

A traditional electrophoretic procedure for detection of Bence–Jones proteinuria, employing Amido black stain on 200-fold concentrated urine, has been compared to two procedures employing highly sensitive protein stains not requiring prior urine concentration. All three procedures were carried out on 80 random urine samples screened for Bence–Jones proteinuria and 10 samples were provided by patients attending a myeloma clinic. A new procedure employing modified Coomassie brilliant blue stain on unconcentrated urine showed comparable sensitivity to the established procedure (82% versus 88%, respectively) and specificity (77% versus 74%, respectively), when assessed against immunofixation as a reference method. However, the new method is considerably quicker and cheaper. A second method, employing Gold stain, showed enhanced sensitivity (94% versus 88% for Amido black) but lower specificity (62% versus 74% for Amido black). However, this method is labour intensive and relatively expensive. Our data suggest that the procedure employing modified Coomassie brilliant blue may be a suitable alternative to the traditional procedure commonly used in many clinical laboratories.

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