Bone Marrow Colony Stimulating Activity from the Isolated Perfused Rat Liver

1974 ◽  
Vol 46 (2) ◽  
pp. 17P-17P
Author(s):  
A. Howell ◽  
W. G. E. Cooksley ◽  
C. J. Danpure
Keyword(s):  
Bone Marrow ◽  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Colony Stimulating Activity

Release of Colony-Stimulating Activity from the Isolated Perfused Rat Liver

1976 ◽  
Vol 50 (6) ◽  
pp. 539-544
Author(s):  
A. Howell ◽  
W. G. E. Cooksley ◽  
C. J. Danpure
Keyword(s):  
Rat Liver ◽  
Colony Formation ◽  
Rat Plasma ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Albumin Synthesis ◽  
Isolated Rat Liver ◽  
Colony Stimulating Activity ◽  
Isolated Rat

1. Colony-stimulating activity appeared in the perfusate of the isolated rat liver during perfusions with either whole rat blood, rat plasma or an artificial perfusate of Eagle's medium and albumin. 2. Dialysable inhibitors of colony formation were also released during perfusions. 3. Colony-stimulating activity in artificial perfusate could be enhanced by the addition of rat plasma in vitro. Concentrations of cycloheximide that inhibited albumin synthesis by the liver did not inhibit the release of colony-stimulating activity.

A Comparison of Plasminogen Activators Derived from Rat Plasma, Primary Rat Hepatocytes and Isolated Perfused Rat Liver

1982 ◽  
Vol 47 (02) ◽  
pp. 166-172 ◽  
Author(s):  
Yoav Sharoni ◽  
Maria C Topal ◽  
Patricia R Tuttle ◽  
Henry Berger
Keyword(s):  
Rat Liver ◽  
Isoelectric Point ◽  
Rat Hepatocytes ◽  
Cell Types ◽  
Plasminogen Activators ◽  
Rat Plasma ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Molecular Weights ◽  
Physiological Relevance

SummaryOf the two cell types it was possible to culture from the dissociated rat liver, hepatocytes and Kupffer cells, only the former were fibrinolytically active. Rat hepatocytes during the first 24 hr in culture secreted two plasminogen activators with molecular weights identical to those found in rat plasma, an 80,000-dalton form (PA-80) and a 45,000-dalton form (PA-45). Partially purified preparations of plasminogen activators from both sources were subjected to isoelectric focusing (IEF) to compare characteristics further. There were three distinct peaks of PA-45 in each preparation with isoelectric points of 7.1, 7.2 and 7.4; all electrophoretic forms had the same low affinity to fibrin. PA-80 from both sources displayed similar IEF profiles with forms ranging from pH values of 7 to 8, all with the same high affinity to fibrin. The major form of PA-80 in the plasma preparation had an isoelectric point of 7.9 whereas that in the hepatocyte preparation had an isoelectric point of 7.6. The isolated perfused rat liver was also shown to produce both PA-80 and PA-45 emphasizing the physiological relevance of the findings with hepatocytes. It is concluded that in the rat hepatocytes contribute to the plasma profile with regard to the plasminogen activator content.

Troxerutin protects the isolated perfused rat liver from a possible lipid peroxidation by coumarin

Phytomedicine ◽  
2005 ◽  
Vol 12 (1-2) ◽  
pp. 52-61 ◽  
Author(s):  
B.S. Adam ◽  
R. Pentz ◽  
C.P. Siegers ◽  
O. Strubelt ◽  
M. Tegtmeier
Keyword(s):  
Lipid Peroxidation ◽  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver

scholarly journals Influence of Kynurenate on Cholesterol and Fatty Acid Synthesis in Isolated Perfused Rat Liver

1973 ◽  
Vol 248 (2) ◽  
pp. 738-739
Author(s):  
Christian A. Barth ◽  
H. Jürgen Hackenschmidt ◽  
Elmar E. Weis ◽  
Karl F.A. Decker
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Synthesis ◽  
Acid Synthesis ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver

scholarly journals Degradation of serum amyloid A by isolated perfused rat liver.

1987 ◽  
Vol 262 (4) ◽  
pp. 1583-1589
Author(s):  
L L Bausserman ◽  
A L Saritelli ◽  
P Van Zuiden ◽  
C J Gollaher ◽  
P N Herbert
Keyword(s):  
Rat Liver ◽  
Serum Amyloid A ◽  
Serum Amyloid ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Amyloid A

scholarly journals The Rate of Creatine Synthesis in the Isolated, Perfused Rat Liver

1962 ◽  
Vol 237 (7) ◽  
pp. 2246-2250
Author(s):  
Georg B. Gerber ◽  
Gisela Gerber ◽  
Thomas R. Koszalka ◽  
Leon L. Miller
Keyword(s):  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver
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