The normally expressed κ immunoglobulin light chain gene repertoire and somatic mutations studied by single-sided specific polymerase chain reaction (PCR); frequent occurrence of features often assigned to autoimmunity

We used the polymerase chain reaction to detect insertions of the transposon Tc1 into mlc-2, one of two Caenorhabditis elegans regulatory myosin light chain genes. Our goals were to develop a general method to identify mutations in any sequenced gene and to establish the phenotype of mlc-2 loss-of-function mutants. The sensitivity of the polymerase chain reaction allowed us to identify nematode populations containing rare Tc1 insertions into mcl-2. mlc-2::Tc1 mutants were subsequently isolated from these populations by a sib selection procedure. We isolated three mutants with Tc1 insertions within the mlc-2 third exon and a fourth strain with Tc1 inserted in nearby noncoding DNA. To demonstrate the generality of our procedure, we isolated two additional mutants with Tc1 insertions within hlh-1, the C. elegans MyoD homolog. All of these mutants are essentially wild type when homozygous. Despite the fact that certain of these mutants have Tc1 inserted within exons of the target gene, these mutations may not be true null alleles. All three of the mlc-2 mutants contain mlc-2 mRNA in which all or part of Tc1 is spliced from the pre-mRNA, leaving small in-frame insertions or deletions in the mature message. There is a remarkable plasticity in the sites used to splice Tc1 from these mlc-2 pre-mRNAs; certain splice sites used in the mutants are very different from typical eukaryotic splice sites.

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Site-selected insertion of the transposon Tc1 into a Caenorhabditis elegans myosin light chain gene

Molecular and Cellular Biology ◽

10.1128/mcb.13.2.902-910.1993 ◽

1993 ◽

Vol 13 (2) ◽

pp. 902-910

Author(s):

A M Rushforth ◽

B Saari ◽

P Anderson

Keyword(s):

Polymerase Chain Reaction ◽

Caenorhabditis Elegans ◽

Light Chain ◽

Myosin Light Chain ◽

Null Alleles ◽

Chain Gene ◽

Splice Sites ◽

Chain Reaction ◽

Light Chain Gene ◽

Polymerase Chain

We used the polymerase chain reaction to detect insertions of the transposon Tc1 into mlc-2, one of two Caenorhabditis elegans regulatory myosin light chain genes. Our goals were to develop a general method to identify mutations in any sequenced gene and to establish the phenotype of mlc-2 loss-of-function mutants. The sensitivity of the polymerase chain reaction allowed us to identify nematode populations containing rare Tc1 insertions into mcl-2. mlc-2::Tc1 mutants were subsequently isolated from these populations by a sib selection procedure. We isolated three mutants with Tc1 insertions within the mlc-2 third exon and a fourth strain with Tc1 inserted in nearby noncoding DNA. To demonstrate the generality of our procedure, we isolated two additional mutants with Tc1 insertions within hlh-1, the C. elegans MyoD homolog. All of these mutants are essentially wild type when homozygous. Despite the fact that certain of these mutants have Tc1 inserted within exons of the target gene, these mutations may not be true null alleles. All three of the mlc-2 mutants contain mlc-2 mRNA in which all or part of Tc1 is spliced from the pre-mRNA, leaving small in-frame insertions or deletions in the mature message. There is a remarkable plasticity in the sites used to splice Tc1 from these mlc-2 pre-mRNAs; certain splice sites used in the mutants are very different from typical eukaryotic splice sites.

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Selection of antigen receptors in splenic marginal-zone lymphoma: further support from the analysis of the immunoglobulin light-chain gene repertoire

Leukemia ◽

10.1038/leu.2012.207 ◽

2012 ◽

Vol 26 (12) ◽

pp. 2567-2569 ◽

Cited By ~ 13

Author(s):

V Bikos ◽

E Stalika ◽

P Baliakas ◽

N Darzentas ◽

Z Davis ◽

...

Keyword(s):

Light Chain ◽

Marginal Zone ◽

Marginal Zone Lymphoma ◽

Chain Gene ◽

Splenic Marginal Zone Lymphoma ◽

Gene Repertoire ◽

Antigen Receptors ◽

Immunoglobulin Light Chain ◽

Light Chain Gene ◽

Selection Of

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Routine identification of four sympatric species of calanoid copepods Pseudocalanus spp. in the Atlantic Arctic using a species-specific polymerase chain reaction

Journal of Oceanological Research ◽

10.29006/1564-2291.jor-2020.48(1).4 ◽

2020 ◽

Vol 48 (1) ◽

pp. 62-72

Author(s):

E. A. Ershova

Keyword(s):

Polymerase Chain Reaction ◽

Life Cycles ◽

The Arctic ◽

Relative Distribution ◽

Chain Reaction ◽

Specific Polymerase Chain Reaction ◽

Routine Identification ◽

Polymerase Chain ◽

Species Specific ◽

Plankton Communities

Сalanoid copepods of the genus Pseudocalanus play an important role in the plankton communities of the Arctic and boreal seas, often dominating in numbers and constituting a significant proportion of the biomass of zooplankton. Despite their high presence and significance in the shelf plankton communities, species-specific studies of the biology of these are significantly hampered by extremely small morphological differences between them, especially at the juvenile stages, at which they are virtually indistinguishable. In this paper, we describe a new, routine and low-cost molecular method for identifying all Pseudocalanus species found in the Atlantic sector of the Arctic: the Arctic P. acuspes, P. minutus and the boreal P. moultoni and P. elongatus, and apply it to describe the relative distribution of these species in four locations of the Arctic and sub-Arctic. With this method, species-specific polymerase chain reaction (ssPCR), mass identification of individuals of any developmental stage, including nauplii, is possible. This method can serve as an excellent tool for studying the species-specific biology of this group, describing their life cycles, as well as monitoring changes in Arctic marine ecosystems under the influence of changing climate.

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