Microsatellite DNA markers reveal genetic differentiation among populations of Glossina palpalis gambiensis collected in the agro-pastoral zone of Sideradougou, Burkina Faso

2000 ◽  
Vol 9 (4) ◽  
pp. 433-439 ◽  
Author(s):  
P. Solano ◽  
S. De La Rocque ◽  
T. De Meeus ◽  
G. Cuny ◽  
G. Duvallet ◽  
...  
Keyword(s):  
Genetic Differentiation ◽  
Burkina Faso ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Glossina Palpalis Gambiensis ◽  
Microsatellite Dna Markers ◽  
Glossina Palpalis

Genetic Evaluation of Black Sea Bream (Acanthopagrus schlegelii) Stock Enhancement in the South China Sea Based on Microsatellite DNA Markers

Fishes ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 47
Author(s):  
Xi Wang ◽  
Zhuoying Weng ◽  
Yang Yang ◽  
Sijie Hua ◽  
Hanfei Zhang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
South China Sea ◽  
Genetic Differentiation ◽  
Black Sea ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Stock Enhancement ◽  
Sea Bream ◽  
Contribution Rate ◽  
Microsatellite Dna Markers

This is the first genetic evaluation of hatchery-based stock enhancement of black sea bream (Acanthopagrus schlegelii) in the South China Sea after a two-year monitoring period. In this study, microsatellite DNA markers were used to calculate the contribution rate and analyze genetic changes before and after stock enhancement. Two out of one hundred and sixty nine individuals from three recaptured populations were assigned to broodstock with a contribution rate of 1.18%, revealing that the hatchery-released juvenile fish could survive in the natural environment and had a positive effect on population replenishment in wild black sea bream abundance. However, we found that the release population had the lowest genetic diversity and significant genetic differentiation from other populations. In addition, genetic diversity detected in the recaptured population was lower than that in the wild population, and their genetic differentiation reached a significant level. Our results suggested that releasing cultured black sea bream juveniles with low genetic quality might be genetically harmful for the maintenance of wild genotypes. Therefore, it is necessary to assess the genetic variation of the hatchery population before implementing a stock enhancement and establish a long-term evaluation for monitoring the genetic effect caused by releasing this fish species.

scholarly journals Admixture analysis of stocked brown trout populations using mapped microsatellite DNA markers: indigenous trout persist in introgressed populations

2009 ◽  
Vol 5 (5) ◽  
pp. 656-659 ◽  
Author(s):  
Michael M. Hansen ◽  
Karen-Lise D. Mensberg
Keyword(s):  
Genetic Differentiation ◽  
Brown Trout ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Indigenous Population ◽  
Population Level ◽  
Captive Populations ◽  
Microsatellite Dna Markers ◽  
Spawning Time ◽  
Clear Identification

Admixture between wild and captive populations is an increasing concern in conservation biology. Understanding the extent of admixture and the processes involved requires identification of admixed and non-admixed individuals. This can be achieved by statistical methods employing Bayesian clustering, but resolution is low if genetic differentiation is weak. Here, we analyse stocked brown trout populations represented by historical (1943–1956) and contemporary (2000s) samples, where genetic differentiation between wild populations and stocked trout is weak (pairwise F ST of 0.047 and 0.053). By analysing a high number of microsatellite DNA markers (50) and making use of linkage map information, we achieve clear identification of admixed and non-admixed trout. Moreover, despite strong population-level admixture by hatchery strain trout in one of the populations (70.8%), non-admixed individuals nevertheless persist (7 out of 53 individuals). These remnants of the indigenous population are characterized by later spawning time than the majority of the admixed individuals. We hypothesize that isolation by time mediated by spawning time differences between wild and hatchery strain trout is a major factor rescuing a part of the indigenous population from introgression.

ANALYSIS OF GENETIC STRUCTURE OF DOGS OF FRENCH BULLDOG BREED USING MICROSATELLITE DNA MARKERS

2018 ◽  
Vol 51 ◽  
pp. 185-192
Author(s):  
S. Kruhlyk ◽  
V. Dzitsiuk ◽  
V. Spyrydonov
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
High Variability ◽  
Molecular Diagnostic ◽  
Allelic Variants ◽  
Microsatellite Dna Markers ◽  
Gene Complexes ◽  
French Bulldog

Genetic variability of domestic dogs is a source for effective process of breed formation and creating unique gene complexes. In the world, for preservation of genetic resources of dogs, there are dog training associations which have great confidence: American Club Dog Breeders (AKC), the British Kennel Club (KC) and the Federation Cynologique Internationale (FCI), aimed at protecting breeding dogs, standards creation, registration of a breed, and issuance of accurate pedigrees. Evaluation of the genetic diversity of dog breeds is able to significantly complement and improve their breeding programs. Since breeds of dog differ in morphological and economic characteristics, the problem of finding of the breed features in the genome of animals is becoming more topical. From this point of view, French Bulldog is an interesting breed of dog (FRANC.BULLDOGGE, FCI standard number 101) which belongs by the classification of breeds, adopted in FCI, to the group IX – a dog-companion for health and fun, but to a subgroup of fighting dogs of a small format. French Bulldog breed has been researched slightly not only in Ukraine and also abroad, as the main work of all dog association is focused on solving theoretical and practical issues of breeding, keeping, feeding, veterinary protection and others. The study was conducted at Research Department of Molecular Diagnostic Tests of Ukrainian Laboratory of Quality and Safety of Agricultural Products. 33 animals of French Bulldog breed, admitted to use in dog breeding of Ukrainian Kennel Union (UKU), were involved for the genetic analysis using DNA markers. The materials of the research were buccal epithelial cells, selected before the morning feeding of animals by scraping mucous membrane of oral cavity with disposable, dry, sterile cotton swab. Genomic DNA was extracted using KIT-set of reagents for DNA isolation according to the manufacturer's instructions. PEZ1, PEZ3, PEZ6, PEZ8, FHC 2010, FHC 2054 markers, recommended by International Society for Animal Genetics (ISAG), ACN, КC and FCI, were used for research. As a result of research 25 alleles for all the loci were detected in the experimental sample of dogs. The average number of alleles at the locus Na, obtained by direct counting, was 4.16. The most polymorphic loci for this breed were PEZ6 and PEZ3 with 8 and 6 allelic variants. Monomorphic loci were PEZ8 and FHC 2054 which had 4 and 3 alleles and the lowest level of polymorphism was observed for PEZ 1 and FHC 2010 loci in which only 2 alleles were identified. On analyzing the molecular genetic characteristics of dogs of French Bulldog breed, we found a high variability of genotype on rare alleles, which included alleles: M, C, D, E, J, K, L, O, N and representing 60% of the total number of the identified alleles. C, D, E alleles for PEZ3 locus and O allele at PEZ6 locus are unique to the sampling of dogs because they are not repeated in other loci. Typical alleles: N, F, R, I, P, K, M are 40% of the total. But F, R alleles for PEZ3 locus and P allele for locus PEZ6 are not repeated either in standard allelic variants or in rare one, indicating a high information content of these alleles and loci to be used for further monitoring of allele pool, genetic certification and identification of dogs. Microsatellite DNA loci were analyzed as a result of investigations of French Bulldogs and the most informative: PEZ3, PEZ6 and PEZ8 were found, which have high efficiency in individual and breed certification of dogs due to high variability. These data allow further monitoring of the state of genetic diversity of the breed and the development of measures for improvement of breeding to preserve the structure of breeding material. The study of individual and population genetic variability is advisable to continue for breeding of French Bulldogs "in purity" and preserving valuable gene complexes. The results are the basis for further monitoring of the proposed informative panels of microsatellite DNA markers for genotyping dog of French Bulldog breed and their complex evaluation.

Genetic diversity of five native Chinese yak breeds based on microsatellite DNA markers

2008 ◽  
Vol 16 (2) ◽  
pp. 156
Author(s):  
Liao Xinjun ◽  
Chang Hong ◽  
Zhang Guixiang ◽  
Wang Donglei ◽  
Song Weitao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Microsatellite Dna Markers

Correlation analysis of microsatellite DNA markers with major growth traits of tilapia (Oreochromis niloticus)

2010 ◽  
Vol 34 (2) ◽  
pp. 169-177
Author(s):  
Fu-ping LIU ◽  
Jun-jie BAI ◽  
Hong-mei SONG ◽  
Xing YE ◽  
Sheng-jie LI ◽  
...  
Keyword(s):  
Correlation Analysis ◽  
Dna Markers ◽  
Oreochromis Niloticus ◽  
Microsatellite Dna ◽  
Growth Traits ◽  
Microsatellite Dna Markers

scholarly journals Characterisation of microsatellite DNA markers for Grevillea globosa C. A. Gardner

2014 ◽  
Vol 6 (3) ◽  
pp. 689-691 ◽  
Author(s):  
Melissa A. Millar ◽  
Margaret Byrne ◽  
David J. Coates ◽  
J. Dale Roberts
Keyword(s):  
Dna Markers ◽  
Microsatellite Dna ◽  
Microsatellite Dna Markers
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