scholarly journals Interaction between the IncHI1 plasmid R27 coupling protein and type IV secretion system: TraG associates with the coiled-coil mating pair formation protein TrhB

2003 ◽  
Vol 49 (1) ◽  
pp. 105-116 ◽  
Author(s):  
Matthew W. Gilmour ◽  
James E. Gunton ◽  
Trevor D. Lawley ◽  
Diane E. Taylor
Keyword(s):  
Coiled Coil ◽  
Secretion System ◽  
Pair Formation ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Mating Pair ◽  
Type Iv ◽  
Coupling Protein

scholarly journals Transcriptional and translational responsiveness of the Neisseria gonorrhoeae type IV secretion system to conditions of host infections

2021 ◽  
Author(s):  
Melanie M. Callaghan ◽  
Amy K. Klimowicz ◽  
Abigail C. Shockey ◽  
John Kane ◽  
Caitlin S. Pepperell ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Protein Levels ◽  
Type Iv ◽  
Coupling Protein ◽  
Host Infection ◽  
System Coupling ◽  
Translational Mechanisms

AbstractThe type IV secretion system of Neisseria gonorrhoeae translocates single-stranded DNA into the extracellular space, facilitating horizontal gene transfer and initiating biofilm formation. Expression of this system has been observed to be low under laboratory conditions, and multiple levels of regulation have been identified. We used a translational fusion of lacZ to traD, the gene for the type IV secretion system coupling protein, to screen for increased type IV secretion system expression. We identified several physiologically relevant conditions, including surface adherence, decreased manganese or iron, and increased zinc or copper, which increase gonococcal type IV secretion system protein levels through transcriptional and/or translational mechanisms. These metal treatments are reminiscent of the conditions in the macrophage phagosome. The ferric uptake regulator, Fur, was found to repress traD transcript levels, but to also have a second role, acting to allow TraD protein levels to increase only in the absence of iron. To better understand type IV secretion system regulation during infection, we examined transcriptomic data from active urethral infection samples from five men. These data demonstrated differential expression of 20 of 21 type IV secretion system genes during infection, indicating upregulation of genes necessary for DNA secretion during host infection.

scholarly journals Transcriptional and translational responsiveness of the Neisseria gonorrhoeae type IV secretion system to conditions of host infections

2021 ◽  
Author(s):  
Melanie M. Callaghan ◽  
Amy K. Klimowicz ◽  
Abigail C. Shockey ◽  
John Kane ◽  
Caitlin S. Pepperell ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Protein Levels ◽  
Type Iv ◽  
Coupling Protein ◽  
Host Infection ◽  
System Coupling ◽  
Translational Mechanisms

The type IV secretion system of Neisseria gonorrhoeae translocates single-stranded DNA into the extracellular space, facilitating horizontal gene transfer and initiating biofilm formation. Expression of this system has been observed to be low under laboratory conditions, and multiple levels of regulation have been identified. We used a translational fusion of lacZ to traD , the gene for the type IV secretion system coupling protein, to screen for increased type IV secretion system expression. We identified several physiologically relevant conditions, including surface adherence, decreased manganese or iron, and increased zinc or copper, which increase gonococcal type IV secretion system protein levels through transcriptional and/or translational mechanisms. These metal treatments are reminiscent of the conditions in the macrophage phagosome. The ferric uptake regulator, Fur, was found to repress traD transcript levels, but to also have a second role, acting to allow TraD protein levels to increase only in the absence of iron. To better understand type IV secretion system regulation during infection, we examined transcriptomic data from active urethral infection samples from five men. These data demonstrated differential expression of 20 of 21 type IV secretion system genes during infection, indicating upregulation of genes necessary for DNA secretion during host infection.

scholarly journals Identification of the DotL coupling protein subcomplex of the Legionella Dot/Icm type IV secretion system

2012 ◽  
Vol 85 (2) ◽  
pp. 378-391 ◽  
Author(s):  
Carr D. Vincent ◽  
Jonathan R. Friedman ◽  
Kwang Cheol Jeong ◽  
Molly C. Sutherland ◽  
Joseph P. Vogel
Keyword(s):  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Type Iv ◽  
Coupling Protein

scholarly journals Conjugative Plasmid Protein TrwB, an Integral Membrane Type IV Secretion System Coupling Protein

2001 ◽  
Vol 277 (9) ◽  
pp. 7556-7566 ◽  
Author(s):  
F. Xavier Gomis-Rüth ◽  
Gabriel Moncalián ◽  
Fernando de la Cruz ◽  
Miquel Coll
Keyword(s):  
Secretion System ◽  
Type Iv Secretion ◽  
Conjugative Plasmid ◽  
Type Iv Secretion System ◽  
Type Iv ◽  
Coupling Protein ◽  
Membrane Type ◽  
System Coupling

High prevalence of functional type IV secretion system for CagA protein in Japanese clinical Helicobacter pylori

2001 ◽  
Vol 120 (5) ◽  
pp. A652-A653
Author(s):  
Y HIRATA ◽  
S MAEDA ◽  
Y MITUNO ◽  
M AKANUMA ◽  
T KAWABE ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Functional Type ◽  
Caga Protein ◽  
Type Iv ◽  
High Prevalence
Sign in / Sign up

Export Citation Format

Share Document