Enumeration of Carnobacterium divergens V41, Carnobacterium piscicola V1 and Lactobacillus brevis LB62 by in situ hybridization-flow cytometry

Polyploidy has played a crucial role in the evolution of many plant taxa, namely in higher latitudinal zones. Surprisingly, after several decades of an intensive research on polyploids, there are still common polyploid species whose evolutionary history is virtually unknown. Here, we addressed the origin of sweet vernal grass (Anthoxanthum odoratum) using flow cytometry, DNA sequencing, and in situ hybridization-based cytogenetic techniques. An allotetraploid and polytopic origin of the species has been verified. The chromosome study reveals an extensive variation between the European populations. In contrast, an autopolyploid origin of the rarer tetraploid vernal grass species, A. alpinum, has been corroborated. Diploid A. alpinum played an essential role in the polyploidization of both European tetraploids studied.

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Telomere length analysis by fluorescence in situ hybridization and flow cytometry (Flow-FISH)/Telomerlängen-Messung mittels Fluoreszenz-in-situ-Hybridisierung und Durchflusszytometrie (Flow-FISH)

LaboratoriumsMedizin ◽

10.1515/labmed.2004.047 ◽

2004 ◽

Vol 28 (4) ◽

pp. 307-316

Author(s):

Ulrike Hartmann ◽

Fabian Beier ◽

Tim H. Brümmendorf

Keyword(s):

Flow Cytometry ◽

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Telomere Length ◽

Length Analysis

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Rapid Detection of Lactobacillus Brevis in Beer Production by the Combination of Microcolony and Fluorescence in Situ Hybridization (FISH)

Journal of the American Society of Brewing Chemists ◽

10.1094/asbcj-2012-0410-01 ◽

2012 ◽

Vol 70 (2) ◽

pp. 91-94

Author(s):

Si Meng ◽

Deliang Wang ◽

Xiaoyu Liu ◽

Yanqing Zhang ◽

Liyun Guo ◽

...

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Rapid Detection ◽

Lactobacillus Brevis ◽

Beer Production

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Correlation of assessment of plasma cells by flow cytometry and detection of cytogenomic abnormalities by fluorescence in situ hybridization in plasma cell neoplasms

International Journal of Laboratory Hematology ◽

10.1111/j.1751-553x.2011.01323.x ◽

2011 ◽

pp. no-no

Author(s):

G. LU ◽

X. X. ZHANG ◽

M. J. YOU ◽

W. CHEN

Keyword(s):

Flow Cytometry ◽

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Plasma Cell ◽

Plasma Cells ◽

Plasma Cell Neoplasms

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Detection of t(14;18)(q32;q21) in B-Cell Chronic Lymphocytic Leukemia

Archives of Pathology & Laboratory Medicine ◽

10.5858/2005-129-410-doqibc ◽

2005 ◽

Vol 129 (3) ◽

pp. 410-411

Author(s):

Wolfgang Kern ◽

Torsten Haferlach ◽

Susanne Schnittger ◽

Claudia Schoch

Keyword(s):

Flow Cytometry ◽

In Situ Hybridization ◽

Chronic Lymphocytic Leukemia ◽

Follicular Lymphoma ◽

B Cell ◽

Lymphocytic Leukemia ◽

Multiparameter Flow Cytometry ◽

Lymphoma Therapy ◽

Cell Chronic Lymphocytic Leukemia

Abstract Cytomorphologic testing and multiparameter flow cytometry are the mainstays in diagnosing B-cell chronic lymphocytic leukemia, whereas fluorescence in situ hybridization that targets the translocation t(14;18)(q32;q21) often is used to identify follicular lymphoma. Therapy is highly diverse between both diseases. We describe a case with cytomorphologically and immunologically proven B-cell chronic lymphocytic leukemia in which t(14;18)(q32;q21) was found.

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Ultrasensitive RNA In Situ Hybridization for Kappa and Lambda Light Chain mRNA in Marginal Zone Lymphoma Demonstrates Comparable Performance to Flow Cytometry

American Journal of Clinical Pathology ◽

10.1093/ajcp/aqz121.011 ◽

2019 ◽

Vol 152 (Supplement_1) ◽

pp. S109-S109

Author(s):

Michael Franklin ◽

Chelsey Deel ◽

Mohammad Vasef

Keyword(s):

Flow Cytometry ◽

In Situ Hybridization ◽

B Cell ◽

Light Chain ◽

Marginal Zone ◽

Marginal Zone Lymphoma ◽

Ffpe Tissue ◽

Light Chains ◽

Light Chain Restriction

Abstract Objectives Evaluation of light chain restriction is critical to establish clonality in B-cell lymphoproliferative disorders (LPDs). Immunohistochemistry (IHC) and in situ hybridization (ISH) are commonly used to assess light chain restriction in formalin-fixed, paraffin-embedded (FFPE) tissues. However, except for cases with plasma cell differentiation, these techniques often fail to identify immunoglobulin light chains. An ultrasensitive technique, RNAscope, has been recently introduced that can identify light chains in cases of B-cell LPDs. We analyzed the utility of this ultrasensitive method in detection of clonality and correlated with flow cytometry results when available. Methods A tissue microarray was constructed using 1.6-mm diameter tissue punches of 31 FFPE tissue blocks from 27 cases that were previously characterized as marginal zone lymphoma (MZL) by a combination of morphology, IHC, and/or flow cytometry. Cases included 8 nodal and 19 extranodal MZLs. In two cases, additional blocks were included to assess reproducibility. For ultrasensitive ISH RNAscope assay, 4-µm thickness tissue sections were hybridized using kappa and lambda probes, incubated overnight, counterstained with hematoxylin, cover-slipped, and reviewed blindly without knowledge of prior flow cytometry results. Results Of 18 cases with evaluable staining, 15 were clonal and 3 were polytypic. Flow cytometry was available in 14 of these 18 cases with concordance in 13 of 14 (93%). The discordant case was polytypic by flow cytometry but kappa restricted by RNAscope. The false-negative flow results could be due to sampling issues. In six cases, staining failed and could not be evaluated. Conclusion Ultrasensitive RNAscope is a reliable assay in the detection of clonality in FFPE tissue, particularly where fresh tissue is not available for flow cytometry. In addition, our results confirm and further expand prior observations that RNAscope is a highly sensitive and specific assay with high concordance with flow cytometry.

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