Mass spectrometry in microbial metabolomic analysis as an analytical tool for dereplication strategy

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
E Crevelin ◽  
L Beraldo de Moraes ◽  
I Soares de Melo
Keyword(s):  
Mass Spectrometry ◽  
Analytical Tool ◽  
Metabolomic Analysis

scholarly journals Brightly coloured tissues in limid bivalves chemically deter predators

2019 ◽  
Vol 6 (10) ◽  
pp. 191298
Author(s):  
Lindsey F. Dougherty ◽  
Alexandria K. Niebergall ◽  
Corey D. Broeckling ◽  
Kevin L. Schauer ◽  
Jingchun Li
Keyword(s):  
Mass Spectrometry ◽  
Chemical Defence ◽  
Chemical Compositions ◽  
Marine Bivalve ◽  
Metabolomic Analysis ◽  
Defence Mechanism ◽  
Defensive Compounds ◽  
Mantis Shrimp ◽  
Metabolite Profiles ◽  
Bivalve Tissues

Members of the marine bivalve family Limidae are known for their bright appearance. In this study, their colourful tissues were examined as a defence mechanism towards predators. We showed that when attacked by the peacock mantis shrimp ( Odontodactylus scyllarus ), the ‘disco’ clam, Ctenoides ales , opened wide to expose brightly coloured tissues to the predator. The predator also significantly preferred to consume the internal, non-colourful clam tissues than the external, colourful tissues. Mass spectrometry-based metabolomic analysis confirmed that colourful tissues had significantly different chemical compositions than the non-colourful ones. The internal, non-colourful tissues had metabolite profiles more similar to an outgroup bivalve than to the species' own colourful external tissues. A number of the compounds that differentiated the colourful tissues from the non-colourful tissues appeared to be peptide-like, which potentially serve as the underlying defensive compounds. This is the first study demonstrating that colourful bivalve tissues are used for chemical defence.

scholarly journals Metabolomic Analysis of Gingival Crevicular Fluid Using Gas Chromatography/Mass Spectrometry

2016 ◽  
Vol 5 (1) ◽  
pp. A0047-A0047 ◽  
Author(s):  
Miho Ozeki ◽  
Takenori Nozaki ◽  
Jun Aoki ◽  
Takeshi Bamba ◽  
Kirk R. Jensen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gingival Crevicular Fluid ◽  
Gas Chromatography Mass Spectrometry ◽  
Metabolomic Analysis ◽  
Chromatography Mass Spectrometry ◽  
Crevicular Fluid

Preliminary comparative deep metabolomic analysis of spermatozoa from zebu and crossbred cattle suggests associations between metabolites, sperm quality and fertility

2021 ◽  
Vol 33 (6) ◽  
pp. 427
Author(s):  
Mohua DasGupta ◽  
Arumugam Kumaresan ◽  
Kaustubh Kishor Saraf ◽  
Gayathree Karthikkeyan ◽  
T. S. Keshava Prasad ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Semen Quality ◽  
Sperm Quality ◽  
Tandem Mass ◽  
Metabolomic Analysis ◽  
Metabolomic Profile ◽  
Crossbred Cattle ◽  
Acetyl Coenzyme A ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Poor semen quality and infertility/subfertility are more frequent in crossbred than zebu bulls. Using a high-throughput liquid chromatography–tandem mass spectrometry (LC-MS/MS)-based approach, we established the preliminary metabolomic profile of crossbred and zebu bull spermatozoa (n=3 bulls each) and identified changes in sperm metabolomics between the two groups. In all, 1732 and 1240 metabolites were detected in zebu and crossbred bull spermatozoa respectively. After excluding exogenous metabolites, 115 and 87 metabolites were found to be unique to zebu and crossbred bull spermatozoa respectively whereas 71 metabolites were common to both. In the normalised data, 49 metabolites were found to be differentially expressed between zebu and crossbred bull spermatozoa. The significantly enriched (P<0.05) pathways in spermatozoa were taurine and hypotaurine metabolism (observed metabolites taurine and hypotaurine) in zebu and glycerophospholipid metabolism (observed metabolites phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine) in crossbred bulls. The abundance of nitroprusside (variable importance in projection (VIP) score >1.5) was downregulated, whereas that of l-cysteine, acetyl coenzyme A and 2′-deoxyribonucleoside 5′-diphosphate (VIP scores >1.0) was upregulated in crossbred bull spermatozoa. In conclusion, this study established the metabolomic profile of zebu and crossbred bull spermatozoa and suggests that aberrations in taurine, hypotaurine and glycerophospholipid metabolism may be associated with the higher incidence of infertility/subfertility in crossbred bulls.

scholarly journals Cell-Wide Survey of Amide-Bonded Lysine Modifications by Using Deacetylase CobB

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Yun Wei ◽  
Wan-Jie Yang ◽  
Qi-Jun Wang ◽  
Peng-Cheng Lin ◽  
Jian-Yuan Zhao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Protein Function ◽  
Gas Chromatography Mass Spectrometry ◽  
Human Tissues ◽  
Metabolomic Analysis ◽  
Dynamic Changes ◽  
Post Translational Modifications ◽  
Covalently Bonded ◽  
Different Origins

Abstract Background Lysine post-translational modifications are important regulators of protein function. Proteomic and biochemical approaches have resulted in identification of several lysine modifications, including acetylation, crotonylation, and succinylation. Here, we developed an approach for surveying amide-bonded lysine modifications in the proteome of human tissues/cells based on the observation that many lysine modifications are amide-bonded and that the Salmonella enterica deacetylase, CobB, is an amidase. Results After the proteome of human tissues/cells was denatured and the non-covalently bonded metabolites were removed by acetone washes, and the amide-bonded modifiers were released by CobB and analyzed using liquid- and/or gas chromatography/mass spectrometry metabolomic analysis. This protocol, which required 3–4 days for completion, was used to qualitatively identify more than 40 documented and unreported lysine modifications from the human proteome and to quantitatively analyze dynamic changes in targeted amide-bonded lysine modifications. Conclusions We developed a method that was capable of monitoring and quantifying amide-bonded lysine modifications in cells of different origins.

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