Genotoxic and antigenotoxic activities of washed water extracts from brown jasmine 105 Thai rice cultivar in human lymphocytes in vitro

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
T Ratanavalachai ◽  
S Tanuchit ◽  
S Thitiorul ◽  
N Lerdvuthisopon ◽  
C Jansom
Keyword(s):  
Human Lymphocytes ◽  
Rice Cultivar ◽  
Water Extracts

Antigenotoxic study of rice extracts from Thai Sung-Yod red rice cultivar in human lymphocytes in vitro

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
T Ratanavalachai ◽  
S Thitiorul ◽  
S Tanuchit ◽  
C Jansom ◽  
S Uttama ◽  
...  
Keyword(s):  
Human Lymphocytes ◽  
Rice Cultivar ◽  
Red Rice

Potentiation of doxorubicin (DXR)'s genotoxic activities by ethanolic extract from Thai Plumbago indica roots (EEPIR) in human lymphocytes in vitro

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
T Ratanavalachai ◽  
S Thitiorul ◽  
S Tanuchit ◽  
A Itharat ◽  
I Sakpakdeejaroen
Keyword(s):  
Human Lymphocytes ◽  
Ethanolic Extract ◽  
Plumbago Indica

Protective effects of Dioscorea membranacea rhizome ethanolic extract against doxorubicin-induced genotoxicity in human lymphocytes in vitro

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
T Ratanavalachai ◽  
S Thitiorul ◽  
A Itharat ◽  
N Runraksa ◽  
S Ruangnoo
Keyword(s):  
Human Lymphocytes ◽  
Ethanolic Extract ◽  
Protective Effects

A synthetic corticosteroid, dexamethasone regulates generation of soluble form of interleukin-6 receptor of human lymphocytes, in vitro

2002 ◽  
Vol 53 (3) ◽  
pp. 307-315 ◽  
Author(s):  
Anna Polgár ◽  
Márta Brózik ◽  
Sára Tóth ◽  
Marcsilla Holub ◽  
A. Falus
Keyword(s):  
Interleukin 6 ◽  
Human Lymphocytes ◽  
Soluble Form ◽  
Interleukin 6 Receptor

A Sensitive Method for Assay of Mixed-Function Oxygenase (p-450 complex) in Cell Culture

1988 ◽  
Vol 15 (3) ◽  
pp. 219-223
Author(s):  
Jørgen Clausen ◽  
Søren Achim Nielsen
Keyword(s):  
Human Lymphocytes ◽  
Cellular Systems ◽  
Metabolic Effects ◽  
Assay Method ◽  
Related Family ◽  
Oxygenase Activity ◽  
Metabolic Transformation ◽  
Mixed Function Oxygenase

The mixed-function oxygenase system involved in the metabolism of drugs and xenobiotics has been extensively studied in various animal species and in various organs (1). It is now apparent that in humans the p-450 complex is one representative of a related family, expressed by 13 c-DNA genes showing approximately 36% similarity between the different subfamilies (2). In order to compare the in vivo and in vitro metabolic effects of drugs and xenobiotics, the induction capabilities of the mixed-function oxygenase must be known. The most sensitive non-isotopic assay system for determination of mixed-function oxygenase activity is the method of Nebert & Gelboin (3,4), which is based on the metabolic transformation of benzo-(a)-pyrene to its fluorescent hydroxyl derivatives (5). However, the levels of the mixed-function oxygenase enzymes in different cellular systems show great variations, with the highest activities in liver cells. Therefore, in order to use human lymphocytes and other cellular systems with low mixed-function oxygenase activities, the assay method for determining oxygenase activity must have the highest possible sensitivity. The present communication is devoted to a study aimed at increasing the sensitivity of Nebert & Gelboin's methods for assay of mixed-function oxygenase subfamilies using benzo-(a)-pyrene as a substrate.

scholarly journals Pharmacological comparison of four biopolymeric natural gums as hemostatic agents for management of bleeding wounds: preliminary in vitro and in vivo results

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Himanshu Kushwah ◽  
Nidhi Sandal ◽  
Meenakshi Chauhan ◽  
Gaurav Mittal
Keyword(s):  
Xanthan Gum ◽  
Guar Gum ◽  
Human Lymphocytes ◽  
Sprague Dawley ◽  
Gum Tragacanth ◽  
Civilian Trauma ◽  
Sprague Dawley Rats ◽  
Cytotoxicity Studies

Abstract Background Uncontrolled bleeding is one of the primary reasons for preventable death in both civilian trauma and military battle field. This study evaluates in vitro and in vivo hemostatic potential of four biopolymeric natural gums, namely, gum tragacanth, guar gum, xanthan gum, and gum acacia. In vitro evaluation of whole blood clotting time and erythrocyte agglutination assay were carried out. In vitro cytotoxicity studies with respect to each gum were done in human lymphocytes to ascertain percent cell viability. In vivo hemostatic potential of each gum (as sponge dressing and powder form) was evaluated in Sprague Dawley rats using tail bleeding assay and compared with commercially available hemostatic sponge. Other important parameters like (a) time taken for complete hemostasis, (b) amount of blood absorbed, (c) adherence strength of developed hemostatic dressing(s), (d) incidence of re-bleeding, and (e) survival of animals were also studied. Results Of the four test gums studied, xanthan gum (@3mg/ml of blood) and gum tragacanth (@35mg/ml of blood) were able to clot blood in least time (58.75±6.408 s and 59.00±2.082 s, respectively) and exhibited very good hemostatic potential in vitro. Except for xanthan gum, all other test gums did not exhibit any significant cytotoxicity at different time points till 24 h. In rat tail bleeding experiments, gum tragacanth sponge dressing and powder achieved hemostasis in least time (156.2±12.86 s and 76±12.55 s, respectively) and much earlier than commercially available product (333.3±38.84 s; p˂0.01). Conclusion Results indicate potential of gum tragacanth to be developed into a suitable hemostatic product.

Sign in / Sign up

Export Citation Format

Share Document