Platelet-Derived NO in Subjects Affected by Type 2 Diabetes with and without Complications: Is there any Relationship with their Offspring?

Abstract Macro- and microvascular complications are currently the principal causes of morbidity and mortality in patients with diabetes mellitus. Aim of this study was to determine if type 2 diabetic patients with nephropathy and coronary artery disease showed altered platelet-derived nitric oxide (NO) production, compared with diabetic subjects without complications, and if this alteration is also present in their diabetic offspring. In this case-control observational study, platelet NO and peroxynitrite content was determined on plasma from 60 male adult type 2 diabetic patients and 60 male offspring type 2 diabetic patients. Plasmatic levels of homocysteine were also determined in the same individuals. Moreover, Western blot analysis of platelet lysates was performed with specific monoclonal antibody for endothelial (eNOS) and inducible (iNOS) nitric oxide synthase. Our study showed a lower piastrinic production of NO in the group of parents without complications (ADH), compared with the group of offspring without complications (YDH) and with the groups of parents with complications. Furthermore, we observed a lower synthesis of peroxynitrite in platelets from the ADH group than in the groups of patients with complications, and in the YDH group compared with all other groups. Subjects from YDH group also showed lower iNOS expression, compared with all other groups. Our data suggest that alterations in nitric oxide metabolism may represent potential risk factors in type 2 diabetes complications, such as nephropathy and cardiovascular diseases, leading to development of new therapeutic strategies in order to delay and prevent the onset of such complications.

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Serum Levels of Progranulin Are Closely Associated with Microvascular Complication in Type 2 Diabetes

Disease Markers ◽

10.1155/2015/357279 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 17

Author(s):

Lin Xu ◽

Bo Zhou ◽

Huixia Li ◽

Jiali Liu ◽

Junhui Du ◽

...

Keyword(s):

Blood Pressure ◽

Type 2 Diabetes ◽

Microvascular Complications ◽

Linear Regression Analysis ◽

Serum Levels ◽

Diabetic Microangiopathy ◽

Diabetic Patients ◽

Type 2 Diabetic Patients ◽

Type 2 Diabetic

Objective. Progranulin (PGRN) was recently introduced as a novel marker of chronic inflammatory response in obesity and type 2 diabetes capable of directly affecting the insulin signaling pathway. This study aimed to investigate the correlation between PGRN and type 2 diabetics with microvascular complications.Methods. PGRN serum levels and glucose metabolism related substance were measured in 84 type 2 diabetic patients with or without microangiopathies and 12 health persons. Further analyses of serum PGRN in different stages of diabetic microangiopathies were conducted.Results. Serum levels of PGRN were markedly higher in type 2 diabetic patients with microangiopathies. PGRN serum levels increased with the progress of diabetic microangiopathies with significantly highest values detectable in clinical diabetic nephropathy (CDN) and proliferative diabetic retinopathy (PDR) groups. Serum PGRN concentrations in all individuals positively and markedly correlated with systolic blood pressure (SBP), diastolic blood pressure (DBP), body mass index (BMI), triglyceride (TG), urinary albumin excretion rate (UAER), blood urea nitrogen (BUN), creatinine (CRE), white blood cell (WBC), disease duration, IL-6, and TNF-α, while correlating negatively and significantly with eGFR. Multiple linear regression analysis showed that only UAER and CRE were independently associated with serum PGRN.Conclusion. PGRN might be considered as a marker for diabetic microangiopathy and its severity.

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Nitric oxide modulation of renal and cardiac hemodynamics in type 2 diabetes

Acta Endocrinologica ◽

10.1530/eje.0.1460687 ◽

2002 ◽

pp. 687-694 ◽

Cited By ~ 12

Author(s):

M Dalla Vestra ◽

D Sacerdoti ◽

G Bombonato ◽

P Fioretto ◽

G Finucci ◽

...

Keyword(s):

Nitric Oxide ◽

Type 2 Diabetes ◽

Cardiac Output ◽

Diabetic Nephropathy ◽

Renal Plasma Flow ◽

Diabetic Patients ◽

Type 2 Diabetic Patients ◽

Control Subjects ◽

Type 2 Diabetic

OBJECTIVE: To evaluate endothelial function in type 2 diabetic patients with and without diabetic nephropathy. METHODS: We studied the effects of systemic infusion of the nitric oxide (NO) synthase inhibitor NG-monomethyl-l-arginine (L-NMMA) on cardiovascular and renal hemodynamics in six type 2 diabetic patients with microalbuminuria (D2-MA), six type 2 diabetic patients with normoalbuminuria (D2-NA) and five control subjects. Both type 2 diabetic patients and control subjects had mild arterial hypertension. RESULTS: L-NMMA infusion decreased the cardiac index in all groups. A reduction in glomerular filtration rate (GFR) and an increase in filtration fraction were observed only in controls. Renal plasma flow decreased in controls and D2-NA patients and renal vascular resistance increased in all groups. CONCLUSIONS: The effect of L-NMMA on cardiac output was similar in controls and type 2 diabetic patients with and without diabetic nephropathy. In contrast, the effect on GFR was impaired in both diabetic groups, suggesting that glomerular NO homeostasis is altered in type 2 diabetes. Moreover the discrepancy, in diabetic patients, between cardiac and renal effects during L-NMMA infusion suggests that the modulation of glomerular hemodynamics is independent from NO-regulated cardiac output.

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HDL & LDL Status in Untreated Newly Detected Type 2 Diabetic Patients

Chattagram Maa-O-Shishu Hospital Medical College Journal ◽

10.3329/cmoshmcj.v14i1.22877 ◽

2015 ◽

Vol 14 (1) ◽

pp. 31-33

Author(s):

Abu Tarek Iqbal ◽

M Jalal Uddin ◽

Md Salehuddin ◽

Hasan Murad

Keyword(s):

Type 2 Diabetes ◽

Risk Factor ◽

Microvascular Complications ◽

Diabetic Patients ◽

Study Group ◽

Type Ii ◽

Newly Diagnosed ◽

Type 2 Diabetic Patients ◽

Type 2 Diabetic

Objective: The study was conducted with a view to find out HDL & LDL status in newly detected untreated Type II diabetic patients. Methods: Study was carried out on 116 (66 male & 50 female) newly diagnosed untreated type 2 diabetes patients aged 40 - 65 years and were selected randomly for study. Fifty (25 male & 25 female) non diabetic subjects aged 40 - 65 years were randomly selected as control. Results: LDL - C was significantly higher (P<.0001) and HDL -C was significantly lower (<.0001) in study group in comparison to control group.Conclusion: Dyslipidemia is commonly found in type - 2 diabetic patients. It is a risk factor for microvascular complications. So it should be controlled effectively. DOI: http://dx.doi.org/10.3329/cmoshmcj.v14i1.22877 Chatt Maa Shi Hosp Med Coll J; Vol.14 (1); Jan 2015; Page 31-33

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Prevalence and Risk Factors of Depression among Adult Type 2 Diabetic Patients at Al-Jemaih Diabetic Center, Dongola, Northern State, Sudan, 2019

Sudan Journal of Medical Sciences ◽

10.18502/sjms.v15i4.8163 ◽

2020 ◽

Author(s):

Mohamed Osman Abdelaziz ◽

Mohamed Ali Alzain ◽

Mohammed Abdalmageed Abdalkhaleg

Keyword(s):

Risk Factors ◽

Type 2 Diabetes ◽

Diabetic Patients ◽

Type 2 Diabetic Patients ◽

Adult Type ◽

Type 2 Diabetics ◽

Northern State ◽

Type 2 Diabetic

Background: Type 2 diabetes and depression are common diseases with bidirectional associations. Depression in diabetics leads to poor adherence to diet and medication, poor glycemic control, reduced quality of life, and increased diabetes-related healthcare expenditure. This study aimed at evaluating diabetic patients with depression to improve the quality of care delivered. Materials and Methods: An institution-based cross-sectional study was conducted among adult type 2 diabetics at Al-Jemaih Diabetic Center, Dongola, in July–December 2019A standardized pretested questionnaire was used to collect data, and the depression subscale of the Patient Health Questionnaire was used to diagnose depression. Chi-square test and logistic regression were used to analyze the data using the Statistical Package for Social Sciences version 25.0. Results: Out of the 407 participants, 282 (69.3%) were females, and more than half were aged between 40 and 59 years. The prevalence of depression among type 2 diabetic patients was 35.6%, ranging from mild (24.3%) to moderate (7.4%), moderately severe (2.2%), and severe depression (1.7%). The risk factors associated with depression in type 2 diabetes were: combining antidiabetic therapy, long duration of diabetes, family history of psychiatric illness, diabetes-related complications, poor glycemic control, and lack of regular exercise. Conclusion: More than a third of type 2 diabetics have depression. Screening diabetic patients for depression, establishing a psychiatric unit at the diabetic center, and community-based studies are recommended. Key words: diabetes mellitus, depression, Dongola, Northern State

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Hba1c Variability Has a Strong Relationship With Peripheral Sensory And Motor Neuropathy in Type-2 Diabetes Mellitus

Journal of Bio-Science ◽

10.3329/jbs.v29i0.54825 ◽

2021 ◽

pp. 93-100

Author(s):

MA Hossain ◽

MK Sarkar ◽

I Mahbub ◽

SMS Islam

Keyword(s):

Type 2 Diabetes ◽

Peripheral Neuropathy ◽

Diabetic Peripheral Neuropathy ◽

Microvascular Complications ◽

Diabetic Patients ◽

Motor Neuropathy ◽

Type 2 Diabetic Patients ◽

Hba1c Variability ◽

Type 2 Diabetic

Diabetic peripheral neuropathy (DPN) is one of the most common long-term microvascular complications of diabetes. This study was undertaken to investigate the association of HbA1c variability with diabetic peripheral neuropathy (sensory and motor) in patients with type-2 diabetes. In this cross-sectional study, a total of 150 type-2 diabetic patients were screened for DPN and undergone quarterly HbA1c measurements during the year preceding enrolment were recruited. DPN was confirmed in patients displaying both clinical manifestations of neuropathy and neurological abnormalities assessment. Among the recruited patients, 24% (n = 36) were found to have DPN, and these patients also presented with a higher HbA1c as compared to the patients without DPN (p<0.05). In addition, the advanced age and longer duration of diabetes were important and significant (p<0.05) risk factors for peripheral neuropathy. Overall study suggests that increased HbA1c level is strongly associated with DPN (sensory and motor) in type-2 diabetic patients and could be considered as a potent indicator for DPN in the recruited patients. J. Bio-Sci. 29(1): 93-100, 2021 (June)

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Impact of a Booklet about Diabetes Genetic Susceptibility and Its Prevention on Attitudes towards Prevention and Perceived Behavioral Change in Patients with Type 2 Diabetes and Their Offspring

Advances in Preventive Medicine ◽

10.4061/2011/365132 ◽

2011 ◽

Vol 2011 ◽

pp. 1-7 ◽

Cited By ~ 7

Author(s):

Masakazu Nishigaki ◽

Eiko Sato ◽

Ryota Ochiai ◽

Taiga Shibayama ◽

Keiko Kazuma

Keyword(s):

Type 2 Diabetes ◽

Genetic Susceptibility ◽

Behavioral Change ◽

Behavioral Changes ◽

Diabetic Patients ◽

Type 2 Diabetic Patients ◽

The Patient’S View ◽

Self Administered Questionnaire ◽

Type 2 Diabetic

Background. Offspring of type 2 diabetic patients are at a high risk of type 2 diabetes. Information on diabetes genetic susceptibility and prevention should be supplied to the offspring.Methods. A six-page booklet on diabetes genetic susceptibility and prevention was distributed to 173 patients who ere ordered to hand it to their offspring. The patients answered a self-administered questionnaire on booklet delivery and attitudinal and behavioral changes toward diabetes and its prevention in themselves and their offspring.Results. Valid responses were obtained from 130 patients. Forty-nine patients had actually handed the booklet. Booklet induces more relief than anxiety. From the patient's view, favorable attitudinal and/or behavioral changes occurred in more than half of the offspring who were delivered the booklet.Conclusion. The booklet worked effectively on attitudes and behaviors toward diabetes and its prevention both in patients and their offspring. However, the effectiveness of patients as information deliverers was limited.

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Awareness of Complications in Diabetic Patients - Study in a Tertiary Care Centre in Kerala

Journal of Evidence Based Medicine and Healthcare ◽

10.18410/jebmh/2021/605 ◽

2021 ◽

Vol 8 (37) ◽

pp. 3334-3338

Author(s):

Satish Kumar

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Tertiary Care ◽

Diabetic Patients ◽

Tertiary Care Centre ◽

Type 2 Diabetic Patients ◽

Complications Of Diabetes ◽

Type 2 Diabetic

BACKGROUND Type 2 diabetes mellitus is common in the Indian population and awareness regarding the disease and its complications is low among the patients with diabetes. There is lack of studies regarding awareness of complications of diabetes among type 2 diabetic patients. The purpose of this study was to assess the awareness of diabetic complications among type 2 diabetic patients. METHODS This is an institutional based observational study. The sample comprised of 150 adult patients with type 2 diabetes mellitus who underwent treatment in the Department of Medicine, Medical College Kottayam and the patient’s age ranged from 18 - 77 years. RESULTS Majority of patients were in the age group 38 to 57 and the sample comprised of 56 % males and 44 % female patients. Out of 150 patients involved in the study, 120 patients (80 %) were aware of the complications of diabetes mellitus. Among the male patients, 70 (83.33 %) and among females, 50 (75.75 %) were aware of the complications in diabetes. In the study sample, 92 (61.33 %) were aware of the foot problems, 120 patients (80 %) were aware of renal complications, 91 patients were aware regarding eye problems of diabetes, 38 (25.33 %) regarding development of hypertension, 62 (41.33 %) regarding heart attack, 42 (28 %) patients were aware regarding development of stroke and 78 patients (52 %) had awareness regarding occurrence of recurrent infections. CONCLUSIONS Awareness regarding diabetes and its complications is fairly good among the diabetic patients in Kerala. Awareness regarding all complications of diabetes was higher among males than females. Providing awareness to type 2 diabetic patients by various educational programs may be of further help to prevent complications and to decrease the mortality and morbidity in type 2 diabetes patients. KEYWORDS Awareness, Diabetes

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Increased Urine IgM and IgG2Levels, Indicating Decreased Glomerular Size Selectivity, Are Not Affected by Dalteparin Therapy in Patients with Type 2 Diabetes

Biochemistry Research International ◽

10.1155/2012/480529 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Ole Torffvit ◽

Majid Kalani ◽

Jan Apelqvist ◽

Björn Eliasson ◽

Jan W. Eriksson ◽

...

Keyword(s):

Type 2 Diabetes ◽

Physiological Saline ◽

Diabetic Patients ◽

Size Selectivity ◽

Type 2 Diabetic Patients ◽

Once Daily ◽

Urinary Levels ◽

Glomerular Size ◽

Type 2 Diabetic

Fifty-four type 2 diabetic patients with neuroischemic foot ulcers were randomised to treatment with 5000 IU of dalteparin, (n=28), or physiological saline, (n=26), once daily until ulcer healing or for a maximum of 6 months. Thirty-three patients had normo-, 15 micro-, and 6 macroalbuminuria. The urinary levels of IgM and IgG2were elevated in 47 and 50 patients, respectively. Elevated urinary levels of IgM and IgG2indicate decreased glomerular size selectivity. Urine IgM levels were associated with IGF-1/IGFBP-1 and IGFBP-1 levels. Dalteparin treatment increased urinary levels of glycosaminoglycans (P<0.001) and serum IGFBP-1 (P<0.05) while no significant effects were seen in any of the other studied parameters. In conclusion, dalteparin therapy in patients with type 2 diabetes had no effects on urinary levels of albumin, IgM, or IgG2despite significantly increased glycosaminoglycans in urine. Elevated urinary levels of IgM and IgG2might be more sensitive markers of renal disease than albuminuria in patients with type 2 diabetes and antihypertensive therapy.

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