Binding of Steroid Hormones and Anabolic Agents to Bovine Sex-Hormone Binding Globulin

1985 ◽  
Vol 17 (09) ◽  
pp. 454-457 ◽  
Author(s):  
K. Shrimanker ◽  
L. Salter ◽  
R. L. S. Patterson
Keyword(s):  
Steroid Hormones ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Anabolic Agents

Efficient eukaryotic expression system for authentic human sex hormone-binding globulin

2001 ◽  
Vol 360 (3) ◽  
pp. 609-615 ◽  
Author(s):  
Jan HILPERT ◽  
Henrik VORUM ◽  
Regina BURMEISTER ◽  
Robert SPOELGEN ◽  
Irina GRISHKOVSKAYA ◽  
...  
Keyword(s):  
Steroid Hormones ◽  
Plasma Membranes ◽  
Recombinant Expression ◽  
Expression System ◽  
Eukaryotic Expression ◽  
Sex Hormone ◽  
Membrane Receptors ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Glycosylation Pattern

Sex hormone-binding globulin (SHBG) is the main carrier for androgens and oestrogens in humans. It mediates the transport of steroid hormones in the circulation and testicular fluid, and regulates their bioavailability to steroid-responsive tissues. In addition, the protein interacts with membrane receptors expressed in target tissues. Binding to the receptors is suspected to facilitate the uptake of steroid hormones and/or elicit cellular signal transduction. The identity of the SHBG receptor has not yet been resolved, in part due to a lack of sufficient quantities of authentic SHBG for receptor purification and molecular characterization. We have successfully addressed this problem by establishing an episomal expression system in human embryonic kidney cells that produces 5mg of fully active human SHBG per litre. The recombinant protein resembles native SHBG in terms of structure, glycosylation pattern and steroid-binding activity. Moreover, the protein interacts with plasma membranes in steroid target tissues, an activity not observed with SHBG from other recombinant expression systems. Thus our studies have removed an important obstacle to the further elucidation of the role SHBG plays in steroid hormone action.

scholarly journals Long-term weight loss maintenance, sex steroid hormones, and sex hormone-binding globulin

2019 ◽  
Vol 26 (4) ◽  
pp. 417-422 ◽  
Author(s):  
Catherine Duggan ◽  
Jean de Dieu Tapsoba ◽  
Frank Stanczyk ◽  
Ching-Yun Wang ◽  
Karen Foster Schubert ◽  
...  
Keyword(s):  
Weight Loss ◽  
Steroid Hormones ◽  
Weight Loss Maintenance ◽  
Sex Steroid Hormones ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Sex Steroid ◽  
Hormone Binding

scholarly journals Profile of Steroid Hormones and sex Hormone-Binding Globulin of Elite Soldiers

2020 ◽  
Vol 8 (3) ◽  
Author(s):  
Thomaz Talarico Neto ◽  
Higino Carlos Hahns Júnior ◽  
Maria Clara Santos Minelli ◽  
José Mauricio Magraner Paixão dos Santos ◽  
Marcos Corrêa Junior ◽  
...  
Keyword(s):  
Steroid Hormones ◽  
Police Officers ◽  
Physiological Stress ◽  
Police Training ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Age Group ◽  
Hormone Binding ◽  
Military Police ◽  
Special Operations

Background: Military police training should characterize the necessary abilities to conduct their activities with excellence. Biochemical parameters have a significant role in the performance of individuals and, therefore, are of crucial importance when trying to improve the teamwork of police officers, particularly for special operations groups. Hence, the use of laboratory analysis in activities that cause great physiological stress is of paramount importance. Objectives: The present study aimed to evaluate the profile of steroid hormones and the sex hormone-binding-globulin of soldiers belonging to a special operations force. Methods: In this cross-sectional study, blood samples of 75 volunteers collected after 12 hours of fasting are analyzed. Data were analyzed using descriptive statistics (i.e., mean and standard deviation) and compared to the reference values for each biomarker, referencing each individual in their age group. Results: Most of the participants presented adequate concentrations for their respective ages in relation to the data analyzed. Even the inclusion of individuals from different age groups did not change the good results found in the group. Conclusions: The positive results found in such a heterogeneous group with respect to age group can be attributed to an extremely rigorous selection process and a training program that elevates them to the rank of elite soldiers of the military police.

Opposite effects of thyroid hormones on binding proteins for steroid hormones (sex hormone-binding globulin and corticosteroid-binding globulin) in humans

1995 ◽  
Vol 132 (5) ◽  
pp. 594-598 ◽  
Author(s):  
Sonia C Dumoulin ◽  
Bertrand P Perret ◽  
Antoine P Bennet ◽  
Philippe J Caron
Keyword(s):  
Thyroid Hormone ◽  
Thyroid Hormones ◽  
Steroid Hormones ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Thyroid Status ◽  
Corticosteroid Binding Globulin ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients

Dumoulin SC, Perret BP, Bennet AP, Caron PJ. Opposite effects of thyroid hormones on binding proteins for steroid hormones (sex hormone-binding globulin and corticosteroid-binding globulin) in humans. Eur J Endocrinol 1995;132:594–8. ISSN 0804–4643 Sex hormone-binding globulin (SHBG) and corticosteroid-binding globulin (CBG) levels were evaluated in euthyroid (N = 111), hyper- (N = 58) and hypothyroid (N = 38) men, in pre- and postmenopausal women (study 1) and in hyper- (N = 24) and hypothyroid (N = 15) patients before and after treatment with carbimazole or levothyroxine therapy (study 2). The SHBG levels are increased in hyper- and decreased in hypothyroid patients, whereas CBG levels are increased in hypo- and decreased in hyperthyroid patients. The SHBG levels are higher in women than in men with similar thyroid status. Plasma SHBG levels are correlated positively whereas CBG levels are correlated negatively with free thyroid hormone concentrations in men as well as women. In hypothyroid patients, SHBG concentrations increased (p < 0.01) and CBG concentrations decreased (p < 0.01) during levothyroxine treatment. In hyperthyroid patients, SHBG concentrations decreased (p < 0.01) and CBG concentrations increased (p < 0.01) during antithyroid treatment. The SHBG and CBG concentrations in treated hypo- and hyperthyroid patients were not significantly different from those of euthyroid controls. Our data indicate that SHBG and CBG levels depend on thyroid status. Corticosteroid-binding globulin is an index of thyroid hormone action at the liver level whose changes are opposite to those of SHBG in hyper- and hypothyroidism. Philippe Caron, Service d'Endocrinologie et Maladies Métaboliques, CHU Rangueil, 1 Avenue J Poulhès, 31054 Toulouse Cedex, France

Evaluation of a new automated electrochemiluminescent sex hormone-binding globulin (SHBG) immunoassay

2005 ◽  
Vol 43 (1) ◽  
Author(s):  
Marijke Reynders ◽  
Ellen Anckaert ◽  
Johan Schiettecatte ◽  
Johan Smitz
Keyword(s):  
Steroid Hormones ◽  
Sex Hormone ◽  
Biologically Active ◽  
Sex Hormone Binding Globulin ◽  
Total Testosterone ◽  
Hormone Binding

AbstractSerum sex hormone-binding globulin (SHBG) regulates the cellular bioavailability of SHBG-bound steroid hormones. Since variations in SHBG levels may affect the concentration of free, i.e., biologically active testosterone in serum, SHBG levels are commonly measured as a supplement to total testosterone determination. The recently developed electrochemiluminescence Elecsys

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