'Click' Bioconjugation of a Well-Defined Synthetic Polymer and a Protein Transduction Domain

2007 ◽  
Vol 60 (6) ◽  
pp. 410 ◽  
Author(s):  
Jean-François Lutz ◽  
Hans G. Börner ◽  
Katja Weichenhan
Keyword(s):  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
Sodium Azide ◽  
Room Temperature ◽  
Synthetic Polymer ◽  
Protein Transduction ◽  
Protein Transduction Domain ◽  
Tat Peptide ◽  
Optimal Coupling ◽  
High Yields

The copper-catalyzed 1,3-dipolar ‘click’ cycloaddition of azides and alkynes was studied to link a model synthetic polymer to a sequence-defined protein transduction domain (PTD). The bromine chain-ends of a well-defined polystyrene (PS) sample synthesized by atom transfer radical polymerization (Mn 2200 g mol–1, Mw/Mn 1.21) were first transformed into azide functions by substitution with sodium azide, and subsequently reacted with an alkyne-functionalized PTD (i.e., the oligopeptide sequence GGYGRKKRRQRRRG, also known as the TAT peptide). The click bioconjugation proceeded successfully at room temperature, thus affording the targeted PS-b-GGYGRKKRRQRRRG bioconjugate in high yields. However, a slight molar excess of polystyrene was required for optimal coupling.

Reverse Atom-Transfer Radical Polymerization at Room Temperature

2001 ◽  
Vol 22 (6) ◽  
pp. 439-443 ◽  
Author(s):  
Wenxin Wang ◽  
Deyue Yan ◽  
Xuling Jiang ◽  
Christophe Detrembleur ◽  
Philippe Lecomte ◽  
...  
Keyword(s):  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
Room Temperature ◽  
Atom Transfer

Facile synthesis of well-controlled poly(glycidyl methacrylate) and its block copolymers via SARA ATRP at room temperature

2015 ◽  
Vol 6 (10) ◽  
pp. 1875-1882 ◽  
Author(s):  
Francisco Catalão ◽  
Joana R. Góis ◽  
A. S. M. Trino ◽  
Arménio C. Serra ◽  
Jorge F. J. Coelho
Keyword(s):  
Block Copolymers ◽  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
Catalytic System ◽  
Glycidyl Methacrylate ◽  
Room Temperature ◽  
Atom Transfer ◽  
Facile Synthesis

The detailed synthesis of poly(glycidyl methacrylate) (PGMA) by atom transfer radical polymerization (ATRP) using a catalytic system of Fe(0)/Cu(ii)Br2 at room temperature is reported.

Surface molecular imprinted polymers based on Mn-doped ZnS quantum dots by atom transfer radical polymerization for a room-temperature phosphorescence probe of bifenthrin

2017 ◽  
Vol 9 (31) ◽  
pp. 4609-4615 ◽  
Author(s):  
Xuelian Wu ◽  
Xiaodong Lv ◽  
Jixiang Wang ◽  
Lin Sun ◽  
Yongsheng Yan
Keyword(s):  
Quantum Dots ◽  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
Room Temperature ◽  
Atom Transfer ◽  
Room Temperature Phosphorescence ◽  
Molecular Imprinted Polymers ◽  
Imprinted Polymers ◽  
Phosphorescence Probe ◽  
Mn Doped

Based on the room-temperature phosphorescence (RTP) peculiarity of Mn-doped ZnS quantum dots (QDs), a bifenthrin sensor was fabricated by coupling molecular imprinted polymers (MIPs) on the surface of silane modified QDs via atom transfer radical polymerization (ATRP).

Photoinduced metal-free atom transfer radical polymerizations: state-of-the-art, mechanistic aspects and applications

2018 ◽  
Vol 9 (14) ◽  
pp. 1757-1762 ◽  
Author(s):  
Gorkem Yilmaz ◽  
Yusuf Yagci
Keyword(s):  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
State Of The Art ◽  
Synthetic Polymer ◽  
Polymer Chemistry ◽  
Macromolecular Structure ◽  
Atom Transfer ◽  
Precise Control ◽  
Metal Free ◽  
Intensive Research

Photoinduced atom transfer radical polymerization has recently been the center of intensive research in synthetic polymer chemistry because of the unique possibility of topological and temporal control in addition to precise control of macromolecular structure offered by conventional ATRP.

Polymerization of Poly(Itaconic Acid) on Surfaces by Atom Transfer Radical Polymerization in Aqueous Solution

2001 ◽  
Vol 710 ◽  
Author(s):  
Amit Y. Sankhe ◽  
Scott M. Husson ◽  
S. Michael Kilbey
Keyword(s):  
Aqueous Solution ◽  
Fourier Transform ◽  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
Itaconic Acid ◽  
Room Temperature ◽  
Layer Growth ◽  
Atom Transfer ◽  
Organometallic Catalyst ◽  
Kinetics Of

ABSTRACTPoly(itaconic acid) (PIA) was grown from surface-tethered initiator sites via atom transfer radical polymerization (ATRP). The surface-tethered PIA layers were grown from hydroxyl-terminated SAMs capped with initiator molecules of 4-(chloromethyl)-benzoylchloride. This polymerization initiator molecule and a copper-based organometallic catalyst allowed tethered PIA chains to be grown via ATRP at room temperature in aqueous solutions. Ellipsometric studies and external-reflection, Fourier-transform infrared spectroscopy (ER-FTIR) confirm the presence and growth of the surface-tethered PIA layer. We describe here how changing the temperature of polymerization alters the layer growth and kinetics of the process, and demonstrate, via ER-FTIR spectroscopy, that these surface-tethered layers do bind cationic dyes through ion-exchange mechanisms.

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