scholarly journals Influence of odorant receptor repertoire on odor perception in humans and fruit flies

2007 ◽  
Vol 104 (13) ◽  
pp. 5614-5619 ◽  
Author(s):  
A. Keller ◽  
L. B. Vosshall
Keyword(s):  
Odorant Receptor ◽  
Fruit Flies ◽  
Odor Perception ◽  
Receptor Repertoire

Odor perception between heterosexual partners: Its association with depression, anxiety, and genetic variation in odorant receptor OR7D4

2011 ◽  
Vol 86 (3) ◽  
pp. 153-157 ◽  
Author(s):  
Silvia Sookoian ◽  
Adriana Burgueño ◽  
Tomas Fernández Gianotti ◽  
Guillermo Marillet ◽  
Carlos Jose Pirola
Keyword(s):  
Genetic Variation ◽  
Odorant Receptor ◽  
Odor Perception

scholarly journals Implications for human odor sensing revealed from the statistics of the odorant-receptor interactions

2018 ◽  
Author(s):  
Ji Hyun Bak ◽  
Seogjoo Jang ◽  
Changbong Hyeon
Keyword(s):  
High Throughput Screening ◽  
Odorant Receptor ◽  
Olfactory Receptors ◽  
Assay Method ◽  
Theoretic Approach ◽  
Screening Assay ◽  
Odor Perception ◽  
Activation Kinetics ◽  
High Throughput Screening Assay ◽  
Human Odor

Binding of odorants to olfactory receptors (ORs) elicits downstream chemical and neural signals, which are further processed to odor perception in the brain. Recently, Mainland et al. [Sci. data, (2015) 2:sdata20152] have measured ≳ 500 pairs of odorant-OR interaction by a high-throughput screening assay method, opening a new avenue to understanding the principles of human odor coding. Here, using a recently developed minimal model for OR activation kinetics [J. Phys. Chem. B (2017) 121, 1304–1311], we characterize the statistics of OR activation by odorants in terms of three empirical parameters: the half-maximum effective concentration EC50, the efficacy, and the basal activity. While the data size of odorants is still limited, the statistics offer meaningful information on the breadth and optimality of the tuning of human ORs to odorants, and allow us to relate the three parameters with the microscopic rate constants and binding affinities that define the OR activation kinetics. Despite the stochastic nature of the response expected at individual OR-odorant level, we assess that the confluence of signals in a neuron released from the multitude of ORs is effectively free of noise and deterministic with respect to changes in odorant concentration. Thus, setting a threshold to the fraction of activated OR copy number for neural spiking binarizes the electrophysiological signal of olfactory sensory neuron, thereby making an information theoretic approach a viable tool in studying the principles of odor perception.

scholarly journals De Novo Transcriptome Identifies Olfactory Genes in Diachasmimorpha longicaudata (Ashmead)

Genes ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 144 ◽  
Author(s):  
Liangde Tang ◽  
Jimin Liu ◽  
Lihui Liu ◽  
Yonghao Yu ◽  
Haiyan Zhao ◽  
...  
Keyword(s):  
De Novo ◽  
Biological Control Agent ◽  
Odorant Receptor ◽  
Parasitoid Wasp ◽  
Control Agent ◽  
Fruit Flies ◽  
Ionotropic Receptor ◽  
Protein Levels ◽  
Diachasmimorpha Longicaudata ◽  
Sensory Neuron Membrane Protein

Diachasmimoorpha longicaudata (Ashmead, D. longicaudata) (Hymenoptera: Braconidae) is a solitary species of parasitoid wasp and widely used in integrated pest management (IPM) programs as a biological control agent in order to suppress tephritid fruit flies of economic importance. Although many studies have investigated the behaviors in the detection of their hosts, little is known of the molecular information of their chemosensory system. We assembled the first transcriptome of D. longgicaudata using transcriptome sequencing and identified 162,621 unigenes for the Ashmead insects in response to fruit flies fed with different fruits (guava, mango, and carambola). We annotated these transcripts on both the gene and protein levels by aligning them to databases (e.g., NR, NT, KEGG, GO, PFAM, UniProt/SwissProt) and prediction software (e.g., SignalP, RNAMMER, TMHMM Sever). CPC2 and MIREAP were used to predict the potential noncoding RNAs and microRNAs, respectively. Based on these annotations, we found 43, 69, 60, 689, 26 and 14 transcripts encoding odorant-binding protein (OBP), chemosensory proteins (CSPs), gustatory receptor (GR), odorant receptor (OR), odorant ionotropic receptor (IR), and sensory neuron membrane protein (SNMP), respectively. Sequence analysis identified the conserved six Cys in OBP sequences and phylogenetic analysis further supported the identification of OBPs and CSPs. Furthermore, 9 OBPs, 13 CSPs, 3 GRs, 4IRs, 25 ORs, and 4 SNMPs were differentially expressed in the insects in response to fruit flies with different scents. These results support that the olfactory genes of the parasitoid wasps were specifically expressed in response to their hosts with different scents. Our findings improve our understanding of the behaviors of insects in the detection of their hosts on the molecular level. More importantly, it provides a valuable resource for D. longicaudata research and will benefit the IPM programs and other researchers in this filed.

scholarly journals The missense of smell: functional variability in the human odorant receptor repertoire

2013 ◽  
Vol 17 (1) ◽  
pp. 114-120 ◽  
Author(s):  
Joel D Mainland ◽  
Andreas Keller ◽  
Yun R Li ◽  
Ting Zhou ◽  
Casey Trimmer ◽  
...  
Keyword(s):  
Odorant Receptor ◽  
Receptor Repertoire

scholarly journals Pheromone reception in fruit flies expressing a moth's odorant receptor

2006 ◽  
Vol 103 (44) ◽  
pp. 16538-16543 ◽  
Author(s):  
Z. Syed ◽  
Y. Ishida ◽  
K. Taylor ◽  
D. A. Kimbrell ◽  
W. S. Leal
Keyword(s):  
Odorant Receptor ◽  
Fruit Flies ◽  
Pheromone Reception

scholarly journals Evolution of herbivory in Drosophilidae linked to loss of behaviors, antennal responses, odorant receptors, and ancestral diet

2015 ◽  
Vol 112 (10) ◽  
pp. 3026-3031 ◽  
Author(s):  
Benjamin Goldman-Huertas ◽  
Robert F. Mitchell ◽  
Richard T. Lapoint ◽  
Cécile P. Faucher ◽  
John G. Hildebrand ◽  
...  
Keyword(s):  
Odorant Receptor ◽  
Green Leaf Volatiles ◽  
Odorant Receptors ◽  
Leaf Volatiles ◽  
Receptor Repertoire ◽  
Key Innovation ◽  
Leaf Mining ◽  
The Family ◽  
Trophic Shifts ◽  
Antennal Responses

Herbivory is a key innovation in insects, yet has only evolved in one-third of living orders. The evolution of herbivory likely involves major behavioral changes mediated by remodeling of canonical chemosensory modules. Herbivorous flies in the genus Scaptomyza (Drosophilidae) are compelling species in which to study the genomic architecture linked to the transition to herbivory because they recently evolved from microbe-feeding ancestors and are closely related to Drosophila melanogaster. We found that Scaptomyza flava, a leaf-mining specialist on plants in the family (Brassicaceae), was not attracted to yeast volatiles in a four-field olfactometer assay, whereas D. melanogaster was strongly attracted to these volatiles. Yeast-associated volatiles, especially short-chain aliphatic esters, elicited strong antennal responses in D. melanogaster, but weak antennal responses in electroantennographic recordings from S. flava. We sequenced the genome of S. flava and characterized this species’ odorant receptor repertoire. Orthologs of odorant receptors, which detect yeast volatiles in D. melanogaster and mediate critical host-choice behavior, were deleted or pseudogenized in the genome of S. flava. These genes were lost step-wise during the evolution of Scaptomyza. Additionally, Scaptomyza has experienced gene duplication and likely positive selection in paralogs of Or67b in D. melanogaster. Olfactory sensory neurons expressing Or67b are sensitive to green-leaf volatiles. Major trophic shifts in insects are associated with chemoreceptor gene loss as recently evolved ecologies shape sensory repertoires.

scholarly journals A First Glimpse of the Mexican Fruit Fly Anastrepha ludens (Diptera: Tephritidae) Antenna Morphology and Proteome in Response to a Proteinaceous Attractant

2020 ◽  
Vol 21 (21) ◽  
pp. 8086
Author(s):  
Eliel Ruiz-May ◽  
Alma Altúzar-Molina ◽  
José M. Elizalde-Contreras ◽  
Jiovanny Arellano-de los Santos ◽  
Juan Monribot-Villanueva ◽  
...  
Keyword(s):  
Odorant Receptor ◽  
Management Strategies ◽  
Fruit Fly ◽  
Anastrepha Ludens ◽  
Mexican Fruit Fly ◽  
Odor Perception ◽  
Related Proteins ◽  
Odorant Binding ◽  
Odor Receptors ◽  
Specific Odor

Anastrepha ludens is a key pest of mangoes and citrus from Texas to Costa Rica but the mechanisms of odorant perception in this species are poorly understood. Detection of volatiles in insects occurs mainly in the antenna, where molecules penetrate sensillum pores and link to soluble proteins in the hemolymph until reaching specific odor receptors that trigger signal transduction and lead to behavioral responses. Scrutinizing the molecular foundation of odorant perception in A. ludens is necessary to improve biorational management strategies against this pest. After exposing adults of three maturity stages to a proteinaceous attractant, we studied antennal morphology and comparative proteomic profiles using nano-LC-MS/MS with tandem mass tags combined with synchronous precursor selection (SPS)-MS3. Antennas from newly emerged flies exhibited dense agglomerations of olfactory sensory neurons. We discovered 4618 unique proteins in the antennas of A. ludens and identified some associated with odor signaling, including odorant-binding and calcium signaling related proteins, the odorant receptor co-receptor (Orco), and putative odorant-degrading enzymes. Antennas of sexually immature flies exhibited the most upregulation of odor perception proteins compared to mature flies exposed to the attractant. This is the first report where critical molecular players are linked to the odor perception mechanism of A. ludens.

scholarly journals The odorant receptor repertoire of teleost fish

BMC Genomics ◽  
2005 ◽  
Vol 6 (1) ◽  
Author(s):  
Tyler S Alioto ◽  
John Ngai
Keyword(s):  
Teleost Fish ◽  
Odorant Receptor ◽  
Receptor Repertoire
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