scholarly journals Inhibition of DNA synthesis in Ehrlich ascites cells by actinomycin D. I. Delayed inhibition by low doses.

1965 ◽  
Vol 54 (3) ◽  
pp. 745-751 ◽  
Author(s):  
R. Baserga ◽  
R. D. Estensen ◽  
R. O. Petersen ◽  
J. P. Layde
Keyword(s):  
Dna Synthesis ◽  
Actinomycin D ◽  
Low Doses ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells ◽  
Inhibition Of Dna Synthesis

Delayed inhibition of DNA synthesis in mouse jejunum by low doses of Actinomycin D

1966 ◽  
Vol 68 (2) ◽  
pp. 177-184 ◽  
Author(s):  
Renato Baserga ◽  
Richard D. Estensen ◽  
Robert O. Petersen
Keyword(s):  
Dna Synthesis ◽  
Actinomycin D ◽  
Low Doses ◽  
Inhibition Of Dna Synthesis

scholarly journals K+/H+-antiporter nigericin arrests DNA synthesis in Ehrlich ascites carcinoma cells

1989 ◽  
Vol 86 (17) ◽  
pp. 6626-6629 ◽  
Author(s):  
L B Margolis ◽  
Y u Novikova I ◽  
I A Rozovskaya ◽  
V P Skulachev
Keyword(s):  
Membrane Potential ◽  
Dna Synthesis ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Low Concentrations ◽  
Ehrlich Ascites ◽  
Inhibition Of Dna Synthesis ◽  
Dna Synthesis Inhibition

Acidification of the cytoplasm of Ehrlich ascites carcinoma cells to pH 6.3 arrests DNA synthesis in these cells. Such an effect can be achieved by incubating the cells at pH 6.2 or by adding low concentrations of the K+/H+ antiporter, the antibiotic nigericin, at neutral pH. Glucose and anaerobiosis potentiate the nigericin effect. The inhibition of DNA synthesis by nigericin occurs without any significant decrease in the ATP concentration and in the mitochondrial membrane potential. The DNA synthesis inhibition is caused neither by a decrease in the intracellular [K+] nor by an increase in the intracellular [Na+] accompanying the nigericin effect (at least at low concentrations of the antibiotic). Nigericin should thus be regarded as a type of a cytostatic primarily affecting intracellular pH.

DNA Synthesis and Incorporation of P32 in Irradiated Ehrlich Ascites Cells.

1957 ◽  
Vol 94 (1) ◽  
pp. 83-86 ◽  
Author(s):  
L. S. Kelly ◽  
J. D. Hirsch ◽  
G. Beach ◽  
N. L. Petrakis
Keyword(s):  
Dna Synthesis ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells

Inhibition of DNA synthesis and cell division by actinomycin D

1966 ◽  
Vol 22 (5) ◽  
pp. 294-295 ◽  
Author(s):  
H. Rothstein ◽  
J. Fortin ◽  
D. Sonneborn
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Actinomycin D ◽  
Inhibition Of Dna Synthesis

DNA Synthesis and Mitosis in Fused Cells

1969 ◽  
Vol 5 (3) ◽  
pp. 645-697
Author(s):  
R. T. JOHNSON ◽  
H. HARRIS
Keyword(s):  
Dna Synthesis ◽  
Hela Cells ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells

Unlike HeLa homokaryons and HeLa-erythrocyte heterokaryons, heterokaryons made by fusing HeLa cells with Ehrlich ascites cells do not, in general, succeed in synchronizing DNA synthesis or mitosis. Indeed, a form of antisynchrony is observed in which most of the Ehrlich nuclei synthesize DNA and most of the HeLa nuclei do not. This anomalous situation appears to be the consequence of competition between the Ehrlich nuclei and the HeLa nuclei for some factor or factors essential for DNA synthesis. In this competition the Ehrlich nuclei are over-whelmingly successful, so that synthesis of DNA in the HeLa nuclei is largely inhibited. The patterns of DNA synthesis in these heterokaryons are thus essentially determined by the proportions of the two kinds of nuclei in the cell.

Changes in Ribonucleic Acid Degrading Enzymes in Ehrlich Ascites Cells During Growth and After Actinomycin D Treatment

1972 ◽  
Vol 50 (3) ◽  
pp. 244-252 ◽  
Author(s):  
Richard G. von Tigerstrom
Keyword(s):  
Acid Phosphatase ◽  
Tumor Cells ◽  
Enzyme Activities ◽  
Actinomycin D ◽  
Inhibitor Activity ◽  
Rnase Inhibitor ◽  
Ehrlich Ascites ◽  
Untreated Tumor ◽  
Ehrlich Ascites Cells ◽  
Rnase Ii

Ehrlich ascites cells were treated with actinomycin D before transplantation. These cells, collected after 7 days, show increased alkaline RNase II, acid RNase II, and phosphodiesterase II activities and low RNase inhibitor activity as compared to untreated tumor cells collected 7 days after transplantation. RNase I, phosphodiesterase I, and acid phosphatase activities were unchanged. Smaller increases in the same enzyme activities, but no change in RNase inhibitor activity, were observed when untreated cells collected after 4 days were compared to untreated cells collected after 7 days of growth. Ehrlich ascites cells collected after 4 days synthesized protein and RNA at a slightly faster rate than those collected after 7 days. Actinomycin D treated cells synthesized protein at a rate identical to that of the control cells; net synthesis of RNA, however, was significantly reduced. One possible reason for this may be the higher RNase and phosphodiesterase activities in actinomycin D treated cells.

4,5′-Dimethylangelicin, a New Very Active Monofunctional Furoeoumarin

1978 ◽  
Vol 33 (3-4) ◽  
pp. 296-298 ◽  
Author(s):  
F. Bordon ◽  
F. Baccichetti ◽  
F. Carlassare
Keyword(s):  
Dna Synthesis ◽  
Tumor Cells ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Strong Inhibition ◽  
Ehrlich Ascites ◽  
Cross Linkage ◽  
Inhibition Of Dna Synthesis ◽  
Very High

4,5′-dimethylangelicin is a new angular furocoumarin that by irradiation at 365 nm shows a very high photo­ reactivity towards DNA in vivo, without forming any cross-linkage; by photosensitization of Ehrlich ascites tumor cells a strong inhibition of DNA synthesis was observed.

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