scholarly journals Ceramide 1-Phosphate Acts as a Positive Allosteric Activator of Group IVA Cytosolic Phospholipase A2α and Enhances the Interaction of the Enzyme with Phosphatidylcholine

2005 ◽  
Vol 280 (18) ◽  
pp. 17601-17607 ◽  
Author(s):  
Preeti Subramanian ◽  
Robert V. Stahelin ◽  
Zdzislaw Szulc ◽  
Alicja Bielawska ◽  
Wonhwa Cho ◽  
...  
Keyword(s):  
Cytosolic Phospholipase ◽  
Group Iva ◽  
Cytosolic Phospholipase A2α ◽  
Ceramide 1 ◽  
Allosteric Activator

scholarly journals Ceramide 1-Phosphate Is Required for the Translocation of Group IVA Cytosolic Phospholipase A2and Prostaglandin Synthesis

2009 ◽  
Vol 284 (39) ◽  
pp. 26897-26907 ◽  
Author(s):  
Nadia F. Lamour ◽  
Preeti Subramanian ◽  
Dayanjan S. Wijesinghe ◽  
Robert V. Stahelin ◽  
Joseph V. Bonventre ◽  
...  
Keyword(s):  
Prostaglandin Synthesis ◽  
Cytosolic Phospholipase ◽  
Group Iva ◽  
Ceramide 1

Ceramide-1-phosphate activates cytosolic phospholipase A2α directly and by PKC pathway

2006 ◽  
Vol 71 (6) ◽  
pp. 850-857 ◽  
Author(s):  
Hiroyuki Nakamura ◽  
Tetsuya Hirabayashi ◽  
Masaya Shimizu ◽  
Toshihiko Murayama
Keyword(s):  
Cytosolic Phospholipase ◽  
Cytosolic Phospholipase A2α ◽  
Ceramide 1

scholarly journals Ceramide-1-phosphate Binds Group IVA Cytosolic Phospholipase a2via a Novel Site in the C2 Domain

2007 ◽  
Vol 282 (28) ◽  
pp. 20467-20474 ◽  
Author(s):  
Robert V. Stahelin ◽  
Preeti Subramanian ◽  
Mohsin Vora ◽  
Wonhwa Cho ◽  
Charles E. Chalfant
Keyword(s):  
C2 Domain ◽  
Cytosolic Phospholipase ◽  
Group Iva ◽  
Ceramide 1

scholarly journals The interaction of ceramide 1-phosphate with group IVA cytosolic phospholipase A2 coordinates acute wound healing and repair

2019 ◽  
Vol 12 (610) ◽  
pp. eaav5918 ◽  
Author(s):  
H. Patrick MacKnight ◽  
Daniel J. Stephenson ◽  
L. Alexis Hoeferlin ◽  
Savannah D. Benusa ◽  
James T. DeLigio ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Dermal Fibroblasts ◽  
Mutant Form ◽  
Collagen Deposition ◽  
Wild Type ◽  
Fibroblast Migration ◽  
Cytosolic Phospholipase ◽  
Group Iva ◽  
Ceramide 1

The sphingolipid ceramide 1-phosphate (C1P) directly binds to and activates group IVA cytosolic phospholipase A2 (cPLA2α) to stimulate the production of eicosanoids. Because eicosanoids are important in wound healing, we examined the repair of skin wounds in knockout (KO) mice lacking cPLA2α and in knock-in (KI) mice in which endogenous cPLA2α was replaced with a mutant form having an ablated C1P interaction site. Wound closure rate was not affected in the KO or KI mice, but wound maturation was enhanced in the KI mice compared to that in wild-type controls. Wounds in KI mice displayed increased infiltration of dermal fibroblasts into the wound environment, increased wound tensile strength, and a higher ratio of type I:type III collagen. In vitro, primary dermal fibroblasts (pDFs) from KI mice showed substantially increased collagen deposition and migration velocity compared to pDFs from wild-type and KO mice. KI mice also showed an altered eicosanoid profile of reduced proinflammatory prostaglandins (PGE2 and TXB2) and an increased abundance of certain hydroxyeicosatetraenoic acid (HETE) species. Specifically, an increase in 5-HETE enhanced dermal fibroblast migration and collagen deposition. This gain-of-function role for the mutant cPLA2α was also linked to the relocalization of cPLA2α and 5-HETE biosynthetic enzymes to the cytoplasm and cytoplasmic vesicles. These findings demonstrate the regulation of key wound-healing mechanisms in vivo by a defined protein-lipid interaction and provide insights into the roles that cPLA2α and eicosanoids play in orchestrating wound repair.

Role of cytosolic phospholipase A2α in cell rounding and cytotoxicity induced by ceramide-1-phosphate via ceramide kinase

2011 ◽  
Vol 512 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Hiroyuki Nakamura ◽  
Eiko Tada ◽  
Tomohiko Makiyama ◽  
Kana Yasufuku ◽  
Toshihiko Murayama
Keyword(s):  
Cell Rounding ◽  
Ceramide Kinase ◽  
Cytosolic Phospholipase ◽  
Cytosolic Phospholipase A2α ◽  
Ceramide 1

C2-di-ethyl-ceramide-1-phosphate as an inhibitor of group IVA cytosolic phospholipase A2

2012 ◽  
Vol 697 (1-3) ◽  
pp. 144-151 ◽  
Author(s):  
Tomohiko Makiyama ◽  
Hiroyuki Nakamura ◽  
Atsushi Nishida ◽  
Toshihiko Murayama
Keyword(s):  
Phospholipase A2 ◽  
Cytosolic Phospholipase A2 ◽  
Cytosolic Phospholipase ◽  
Group Iva ◽  
Ceramide 1

scholarly journals A Phospholipid Substrate Molecule Residing in the Membrane Surface Mediates Opening of the Lid Region in Group IVA Cytosolic Phospholipase A2

2008 ◽  
Vol 283 (45) ◽  
pp. 31227-31236 ◽  
Author(s):  
John E. Burke ◽  
Yuan-Hao Hsu ◽  
Raymond A. Deems ◽  
Sheng Li ◽  
Virgil L. Woods ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Membrane Surface ◽  
Cytosolic Phospholipase A2 ◽  
Substrate Molecule ◽  
Cytosolic Phospholipase ◽  
Group Iva
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