scholarly journals The ribosomal maturation factor P fromMycobacterium smegmatisfacilitates the ribosomal biogenesis by binding to the small ribosomal protein S12

2018 ◽  
Vol 294 (1) ◽  
pp. 372-378 ◽  
Author(s):  
Tinyi Chu ◽  
Xing Weng ◽  
Carmen Oi Kwan Law ◽  
Hoi-Kuan Kong ◽  
Jeffrey Lau ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Ribosomal Biogenesis ◽  
Maturation Factor ◽  
Ribosomal Protein S12

scholarly journals Cloning and sequencing of mouse ribosomal protein S12 cDNA

1989 ◽  
Vol 17 (16) ◽  
pp. 6722-6722 ◽  
Author(s):  
Mohamed Ayane ◽  
Peter Nielsen ◽  
Georges Köhler
Keyword(s):  
Ribosomal Protein ◽  
Cloning And Sequencing ◽  
Ribosomal Protein S12

scholarly journals Perturbation of 60 S Ribosomal Biogenesis Results in Ribosomal Protein L5- and L11-dependent p53 Activation

2010 ◽  
Vol 285 (33) ◽  
pp. 25812-25821 ◽  
Author(s):  
Xiao-Xin Sun ◽  
Yue-Gang Wang ◽  
Dimitris P. Xirodimas ◽  
Mu-Shui Dai
Keyword(s):  
Ribosomal Protein ◽  
Ribosomal Biogenesis ◽  
P53 Activation ◽  
Ribosomal Protein L5

An intergenic G-rich region in Leishmania tarentolae kinetoplast maxicircle DNA is a pan-edited cryptogene encoding ribosomal protein S12

1992 ◽  
Vol 12 (1) ◽  
pp. 56-67
Author(s):  
D A Maslov ◽  
N R Sturm ◽  
B M Niner ◽  
E S Gruszynski ◽  
M Peris ◽  
...  
Keyword(s):  
Amino Acid ◽  
Ribosomal Protein ◽  
Ribosomal Proteins ◽  
Sequence Similarity ◽  
Rich Region ◽  
Leishmania Tarentolae ◽  
Significant Sequence Similarity ◽  
The Family ◽  
Intergenic Regions ◽  
Ribosomal Protein S12

Six short G-rich intergenic regions in the maxicircle of Leishmania tarentolae are conserved in location and polarity in two other kinetoplastid species. We show here that G-rich region 6 (G6) represents a pan-edited cryptogene which contains at least two domains edited independently in a 3'-to-5' manner connected by short unedited regions. In the completely edited RNA, 117 uridines are added at 49 sites and 32 uridines are deleted at 13 sites, creating a translated 85-amino-acid polypeptide. Similar polypeptides are probably encoded by pan-edited G6 transcripts in two other species. The G6 polypeptide has significant sequence similarity to the family of S12 ribosomal proteins. A minicircle-encoded gRNA overlaps 12 editing sites in G6 mRNA, and chimeric gRNA/mRNA molecules were shown to exist, in agreement with the transesterification model for editing.

scholarly journals The enigma of the gene coding for ribosomal protein S12 in the chtoroplasts of Nicotiana

1986 ◽  
Vol 14 (2) ◽  
pp. 883-898 ◽  
Author(s):  
H. Fromm ◽  
M. Edelman ◽  
B. Koller ◽  
P. Goloubinoff ◽  
E. Galun
Keyword(s):  
Ribosomal Protein ◽  
Gene Coding ◽  
Ribosomal Protein S12

Modulation of 16S rRNA function by ribosomal protein S12

2007 ◽  
Vol 1769 (7-8) ◽  
pp. 462-471 ◽  
Author(s):  
Anton Vila-Sanjurjo ◽  
Ying Lu ◽  
Jamie L. Aragonez ◽  
Rebekah E. Starkweather ◽  
Manoj Sasikumar ◽  
...  
Keyword(s):  
16S Rrna ◽  
Ribosomal Protein ◽  
Ribosomal Protein S12

Bone Marrow Cells from Patients with Shwachman-Diamond Syndrome Abnormally Express Ribosomal Protein and Ribosomal Biogenesis-Related Genes.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3303-3303
Author(s):  
Piya Rujkijyanont ◽  
Joseph Beyene ◽  
Yigal Dror
Keyword(s):  
Gene Expression ◽  
Ribosomal Protein ◽  
Rna Processing ◽  
Real Time Pcr ◽  
Ribosomal Proteins ◽  
Ribosomal Biogenesis ◽  
Rrna Processing ◽  
Rrna Transcription ◽  
Shwachman Diamond Syndrome ◽  
Marrow Cells

Abstract Background and rational: Shwachman-Diamond syndrome (SDS) is an autosomal recessive disorder characterized by varying degrees of cytopenia and high propensity for myelodysplastic syndrome and acute leukemia. SBDS, the gene associated with SDS, has recently been identified and is postulated to play a role in ribosomal biogenesis and RNA processing, but its functions are still unknown. Defects in ribosomal biogenesis can be characterized by abnormal synthesis of rRNA synthesis or ribosomal proteins or both. Determining the mRNA expression pattern of the various RP genes in SBDS deficient cells will help deciphering the role of SBDS in ribosomal biogenesis. Objectives: To determine whether the primary SDS marrow cells which carry homozygous SBDS mutations abnormally express genes which code for ribosomal proteins (RP) or for proteins that are involved in its transcription. Methods: Total RNA from marrow cells from 9 SDS patients who had hypocellular marrow with normal differential and no malignant transformation and 7 healthy age-matched donors of bone marrows for transplantation was extracted. RNA was labeled and hybridized to Affymetrix HG_U133_Plus2.0 GeneChip. Data were pre-processed using robust multichip analysis (RMA) and differentially expressed genes were identified with permutation-based methods. False discovery rate (FDR)-adjusted p-values were used to rank genes and cluster analysis grouped genes and samples. T-statistic values were used to screen for differentially expressed RP-related genes. Real-time PCR was performed to confirm differential expression of genes found by oligonucleotide microarray. Results: Of the 38,500 genes on the HG_133_Plus2.0 we analyzed 375 known ribosomal protein and RNA processing-related genes. Interestingly, there were differences in the expression pattern of the RP genes, suggesting differential regulation of these genes in Sbds-deficient cells. Interestingly, despite uniform decrease in RP gene expression in reduced cell growth conditions, only 27 of the 85 RP genes were downregulated. Downregulation of representative 2 genes was confirmed by real-time PCR. Further, one of the RP genes, RPL27L was upregulated. This gene, which is a target of p53, has a non-ribosomal function and lead to accelerated apoptosis. It is noteworthy that several genes involved in mRNA transcription such as GABPA and YY1were downregulated without dysregulation of genes involved in mRNA degradation, suggesting that the downregulation of the RP gene expression is at the transcription level. In addition to dysregulation of the RP mRNA we also found dysregulation of genes involved in rRNA transcription (e.g. MKI67IP) and pre rRNA processing (e.g. FBL). Conclusions: SBDS-deficiency results in dysregulation of selective group of RP genes as well as genes related to rRNA processing and rRNA transcription. Future studies should focus on the mechanism of the abnormal expression as well as its biological consequences.

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