Nuclear DNA content of the whitefly Bemisia tabaci (Aleyrodidae: Hemiptera) estimated by flow cytometry

2005 ◽  
Vol 95 (4) ◽  
pp. 309-312 ◽  
Author(s):  
J.K. Brown ◽  
G.M. Lambert ◽  
M. Ghanim ◽  
H. Czosnek ◽  
D.W. Galbraith
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Bemisia Tabaci ◽  
Dna Content ◽  
Blood Cells ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Chromosome Counting ◽  
Male And Female ◽  
Haploid Genome Size

AbstractThe nuclear DNA content of the whitefly Bemisia tabaci (Gennnadius) was estimated using flow cytometry. Male and female nuclei were stained with propidium iodide and their DNA content was estimated using chicken red blood cells and Arabidopsis thaliana L. (Brassicaceae) as external standards. The estimated nuclear DNA content of male and female B. tabaci was 1.04 and 2.06 pg, respectively. These results corroborated previous reports based on chromosome counting, which showed that B. tabaci males are haploid and females are diploid. Conversion between DNA content and genome size (1 pg DNA = 980 Mbp) indicate that the haploid genome size of B. tabaci is 1020 Mbp, which is approximately five times the size of the genome of the fruitfly Drosophila melanogaster Meigen. These results provide an important baseline that will facilitate genomics-based research for the B. tabaci complex.

scholarly journals Genome Sizes in Hepatica Mill: (Ranunculaceae) Show a Loss of DNA, Not a Gain, in Polyploids

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
B. J. M. Zonneveld
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Dna Content ◽  
Close Relationships ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Dna Contents ◽  
Asiatic Species ◽  
Nuclear Dna Contents ◽  
Allotetraploid Species

Genome size (C-value) was applied anew to investigate the relationships within the genus Hepatica (Ranunculaceae). More than 50 samples representing all species (except H. falconeri), from wild and cultivated material, were investigated. Species of Hepatica turn out to be diploid (), tetraploid ( ), and a possible pentaploid. The somatic nuclear DNA contents (2C-value), as measured by flow cytometry with propidium iodide, were shown to range from 33 to 80 pg. The Asiatic and American species, often considered subspecies of H. nobilis, could be clearly distinguished from European H. nobilis. DNA content confirmed the close relationships in the Asiatic species, and these are here considered as subspecies of H. asiatica. Parents for the allotetraploid species could be suggested based on their nuclear DNA content. Contrary to the increase in genome size suggested earlier for Hepatica, a significant (6%–14%) loss of nuclear DNA in the natural allopolyploids was found.

scholarly journals Evolution of Genome Size in Duckweeds (Lemnaceae)

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Wenqin Wang ◽  
Randall A. Kerstetter ◽  
Todd P. Michael
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Propidium Iodide ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Continuous Increase ◽  
Spirodela Polyrhiza ◽  
Wolffia Arrhiza ◽  
Basic Reference

To extensively estimate the DNA content and to provide a basic reference for duckweed genome sequence research, the nuclear DNA content for 115 different accessions of 23 duckweed species was measured by flow cytometry (FCM) stained with propidium iodide as DNA stain. The 1C-value of DNA content in duckweed family varied nearly thirteen-fold, ranging from 150 megabases (Mbp) in Spirodela polyrhiza to 1,881 Mbp in Wolffia arrhiza. There is a continuous increase of DNA content in Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia that parallels a morphological reduction in size. There is a significant intraspecific variation in the genus Lemna. However, no such variation was found in other studied species with multiple accessions of genera Spirodela, Landoltia, Wolffiella, and Wolffia.

scholarly journals NUCLEAR DNA CONTENT IN BLUEBERRY DETERMINED BY FLOW CYTOMETRY

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 580e-580
Author(s):  
Rodomiro Ortiz ◽  
D.E. Costich ◽  
T.P. Meagher ◽  
N. Vorsa
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Haploid Genome ◽  
Dna Flow Cytometry ◽  
Dna Amount ◽  
Ploidy Levels ◽  
Polyploid Evolution ◽  
Haploid Genome Size

DNA flow cytometry was used to determine nuclear DNA content in diploid blueberry species, and 3x, 4x, 5x, and 6x ploidy levels. Relative fluorescence intensity of stained nuclei measured by flow cytometry was a function of the number of chromosome sets (X): Y = 3.7X – 2.3 (r2 = 95.1%). DNA flow cytometry should be useful for ploidy level determination in the seedling stage. A significant linear relationship was established between nuclear DNA content and number of chromosomes (x); DNA (pg) = 0.52 x1 (r2 = 99.8%). Based on this equation the haploid genome DNA amount (1C) was calculated as 0.62 ± 0.08 pg, with an approximate haploid genome size of 602 Mbp/1C. The results indicate that conventional polyploid evolution occured in the section Cyanococcus, genus Vaccinium: the increase in DNA was concurrent with increase in chromosome number. DNA content differences among 2x species were correlated with Nei's genetic distance estimates based on 20 isozyme markers. Most of the variation was among species (49%), with 26% between populations within species, and 25% within populations.

Genome size, fluorochrome banding, and karyotype evolution in some Hypochoeris species

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 689-695 ◽  
Author(s):  
M. Cerbah ◽  
J. Coulaud ◽  
B. Godelle ◽  
S. Siljak-Yakovlev
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Nucleolar Organizer ◽  
Phylogenetic Position ◽  
Nucleolar Organizer Regions ◽  
Fluorochrome Banding ◽  
South American

Four South American and two European species of Hypochoeris (Asteraceae) were studied using fluorochrome banding, and genome size was determined by flow cytometry, in order to obtain information about microevolution in this genus and about its primary origin. Fluorochrome banding patterns showed GC-rich repeated sequences, particularly around the nucleolar organizer regions. Few differences appeared among the South American species. Nevertheless, determination of nuclear DNA content and base composition revealed significant differences among these species. The phylogenetic position of Hypochoeris robertia, which has the smallest DNA content, is discussed with regard to chromosome evolution in this genus.Key words: Hypochoeris, Asteraceae, fluorochromes, flow cytometry, nucleolar organizer regions, microevolution.

Genome size differences in Hyalella cryptic species

Genome ◽  
2012 ◽  
Vol 55 (2) ◽  
pp. 134-139 ◽  
Author(s):  
Roland Vergilino ◽  
Kaven Dionne ◽  
Christian Nozais ◽  
France Dufresne ◽  
Claude Belzile
Keyword(s):  
Flow Cytometry ◽  
Cryptic Species ◽  
Dna Barcoding ◽  
Genome Size ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Complex Species ◽  
Numerous Species ◽  
Freshwater Habitats

The Hyalella azteca (Saussure) complex includes numerous amphipod cryptic species in freshwater habitats in America as revealed by DNA barcoding surveys. Two ecomorphs (small and large) have evolved numerous times in this complex. Few phenotypic criteria have been found to differentiate between the numerous species of this complex. The present study aims to explore genome size differences between some species of the H. azteca complex co-occurring in a Canadian boreal lake using flow cytometry. Nuclear DNA content was estimated for 50 individuals belonging to six COI haplotypes corresponding to four provisional species of the H. azteca complex. Species from the large ecomorph had C-values significantly larger than species from the small ecomorph, whereas slight differences were found among species of the small ecomorph. These differences in genome sizes might be linked to ecological and physiological differences among species of the H. azteca complex.

Analysis of intraspecific nuclear DNA content variation in Gleditsiatriacanthos by flow cytometry

1995 ◽  
Vol 25 (3) ◽  
pp. 440-445
Author(s):  
CM. Wake ◽  
P.R. Schaefer ◽  
L.K. Jost ◽  
D.P. Evenson
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Dna Content ◽  
Blood Cells ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Evolutionary Changes ◽  
Wide Range ◽  
Dna Content Variation ◽  
Angiosperm Species

Honeylocust trees (Gleditsiatriacanthos L.) are distributed across a wide range of climatic and edaphic conditions, demonstrating considerable variation in cold, drought, and salinity tolerances. In many angiosperm species, an evolutionary change in nuclear DNA content accompanies colonization into more stressful environments. Fresh leaf nuclei were isolated from 22 honeylocust sources from across the species' native range, mixed with trout red blood cells, stained with propidium iodide, and their DNA content was measured by flow cytometry. According to DNA stainability of the trout red blood cells, leaf nuclei had a DNA content of 1.72 ± 0.02 pg (mean ± SD). Variation in DNA content among the sources was not significant (p < 0.05), suggesting that adaptive evolutionary changes in honeylocust are not evident in increasing or decreasing amounts of leaf nuclear DNA content.

scholarly journals Estimating Nuclear DNA Content in Peach and Related Diploid Species Using Laser Flow Cytometry and DNA Hybridization

1994 ◽  
Vol 119 (6) ◽  
pp. 1312-1316 ◽  
Author(s):  
W. Vance Baird ◽  
Agnes S. Estager ◽  
John K. Wells
Keyword(s):  
Flow Cytometry ◽  
Chromosome Number ◽  
Genome Size ◽  
Dna Content ◽  
Nuclear Dna ◽  
Diploid Species ◽  
Nuclear Genome ◽  
Nuclear Dna Content ◽  
Gene Copy ◽  
Polyploid Series

Using laser flow cytometry, nuclear DNA amounts were estimated for 12 Prunus species, representing three subgenera [Prunophora (Prunus), Amygdalus, and Cerasus (Lithocerasus)], two interspecific hybrids, four cultivars, and a synthetic polyploid series of peach consisting of haploids, diploids, triploids, and tetraploids (periclinal cytochimeras). Peach nuclear DNA content ranged from 0.30 pg for the haploid nuclei to 1.23 pg for the tetraploid nuclei. The diploid genome of peach is relatively small and was estimated to be 0.60±0.03 pg (or 5.8×108 nucleotide base pairs). The polyploid series represented the expected arithmetic progression, as genome size positively correlated with ploidy level (i.e., DNA content was proportional to chromosome number). The DNA content for the 12 diploid species and two interspecific diploid hybrids ranged from 0.57 to 0.79 pg. Genome size estimates were verified independently by Southern blot analysis, using restriction fragment length polymorphism clones as gene-copy equivalents. Thus, a relatively small and stable nuclear genome typifies the Prunus species investigated, consistent with their low, basic chromosome number (× = 8).

scholarly journals Determination of DNA content and relative 2C genome sizes of some promising commercial varieties of sugarcane using flow cytometer

2017 ◽  
Vol 8 ◽  
Author(s):  
A. Mondal S.K. Ghosal ◽  
T. Pal Kalyan Kumar De
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Cane Yield ◽  
Sugarcane Varieties ◽  
Yield Information ◽  
A Genome ◽  
2C Dna Content

<p>In the present study, 2C DNA content and the genome sizes (in picograms-pg and megabase pairs-Mbp respectively) of 19 promising commercial varieties of sugarcane, the derivatives of man-made interspecific hybrids between cultivated and wild species were analyzed using flow cytometry. In this work, 2C nuclear DNA content was determined. Knowing the 2C nuclear DNA content, the unknown chromosome numbers of the varieties could be predicted. Large differences (65 % variation) in DNA content (2C) of 19 varieties were detected, ranging, from 3.80 pg to 10.96 pg, which corresponds to a genome size ranging from 3724.00 Mbp to 10740.80 Mbp due to the variation of ploidy level and are considered the most complex genomes among crop plants. However, the relationship between chromosome number and genome size was highly significant (P &lt; 0.001). In the present study, internode diameter, Sugar juice content and cane yield/ha are also positively correlated with DNA content. The estimated genome sizes would also yield information critical for sugarcane breeding and genome sequencing programs.                                </p><p><strong>Keywords</strong><strong>: </strong>Genome size, Sugarcane varieties, Flow cytometry, DNA content.</p>

Genome size in 21 Artemisia L. species (Asteraceae, Anthemideae): Systematic, evolutionary, and ecological implications

Genome ◽  
2001 ◽  
Vol 44 (2) ◽  
pp. 231-238 ◽  
Author(s):  
Montserrat Torrell ◽  
Joan Vallès
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Life Forms ◽  
Dna Amount ◽  
Ploidy Levels ◽  
Ecological Selection ◽  
Ecological Implications

Genome size was estimated by flow cytometry in 24 populations belonging to 22 Artemisia taxa (21 species, 1 with two subspecies), which represent the distinct subgenera, life forms, basic chromosome numbers, and ploidy levels in the genus. 2C nuclear DNA content values range from 3.5 to 25.65 pg, which represents a more than sevenfold variation. DNA content per haploid genome ranges from 1.75 to 5.76 pg. DNA amount is very well correlated with karyotype length and ploidy level. Some variations in genome size have systematic and evolutionary implications, whereas others are linked to ecological selection pressures.Key words: Artemisia, Asteraceae, flow cytometry, genome size, nuclear DNA amount variation, systematics, evolution, ecology.

Nuclear DNA content of commercially important Eucalyptus species and hybrids

1994 ◽  
Vol 24 (5) ◽  
pp. 1074-1078 ◽  
Author(s):  
D. Grattapaglia ◽  
H.D. Bradshaw Jr.
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Positional Cloning ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Eucalyptus Species ◽  
Ploidy Levels ◽  
2C Dna Content ◽  
Haploid Genome Size ◽  
Commercially Important

This paper reports the nuclear DNA content estimates obtained by flow cytometry for a group of twelve Eucalyptus species and five fast-growing hybrids that includes those most widely planted throughout the world. Estimates of nuclear (2C) DNA content for the species surveyed ranged from 0.77 pg/2C for Eucalyptuscitriodora Hook. (subgenus Corymbia) to 1.47 pg/2C for Eucalyptussaligna Smith (subgenus Symphyomyrtus). This range corresponds to a haploid genome size range of 370–700 megabase pairs. The average physical equivalent of a 1 cM distance could be as low as 200 kilobase pairs in Eucalyptus, an attractive feature for positional cloning efforts in woody plants. The closer the species were in phylogenetic relationship the more similar were their nuclear DNA content values. All the interspecific hybrids surveyed displayed a nuclear DNA content in the expected intermediate range between the respective parental species, with the exception of one originating from Rio Claro, Brazil, whose exact parentage is unknown. No evidence of polyploidy was observed in any of the hybrids. The flow cytometry procedure employed in this study is an efficient method for investigating ploidy levels of high yielding hybrids of Eucalyptus.

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