scholarly journals The effects of some dietary changes upon the concentrations of serum lipids in rats

1971 ◽  
Vol 25 (2) ◽  
pp. 213-224 ◽  
Author(s):  
D. Mcgregor
Keyword(s):  
Serum Lipids ◽  
Wheat Gluten ◽  
Dietary Cholesterol ◽  
Male Rats ◽  
Groundnut Oil ◽  
Adult Rats ◽  
Serum Triglycerides ◽  
Rat Serum ◽  
Lipid Source ◽  
Weanling Rats

1. The responses of serum lipids of weanling and adult male rats to different diets have been examined. Total cholesterol, phospholipids and triglycerides were measured. The proteins used in the diets were casein and wheat gluten, the carbohydrates were starch and sucrose, and the main lipid source was groundnut oil supplemented with either gluten oil or maize oil.2. In general, the type of carbohydrate did not influence the experimental results, whereas the type of protein had a marked effect. Compared with casein, gluten caused an increase in concentration of serum triglycerides, phospholipids and cholesterol in weanling rats, but there was no change in adult animals. Much of this ‘gluten effect’ in weanlings was not ob- served when the diet was supplemented with 0.7% L-lysine hydrochloride.3. When 1% cholesterol was added to the gluten diets, the serum cholesterol concentrations were only slightly and variably affected in weanlings. Serum triglycerides and phospholipids were not altered in adult rats by the dietary cholesterol, whereas there were reductions in these lipid components in the weanlings, whether the protein was supplemented with L-lysine hydrochloride or not.4. Gluten oil was not shown to be responsible for any major portion of the differences observed between gluten and casein in their effects upon the lipids of rat serum.

scholarly journals Interaction of dietary protein, cholesterol and age on lipid metabolism of the rat

1989 ◽  
Vol 61 (3) ◽  
pp. 531-543 ◽  
Author(s):  
Yong-Soon Choi ◽  
Shoichiro Goto ◽  
Ikuo Ikeda ◽  
Michihiro Sugano
Keyword(s):  
Liver Microsomes ◽  
Dietary Cholesterol ◽  
Cholesterol Homeostasis ◽  
Male Rats ◽  
Liver Cholesterol ◽  
Adult Rats ◽  
Young Rats ◽  
Age Related ◽  
Coa Reductase ◽  
Hydroxymethylglutaryl Coa Reductase

1. Male rats at 1 (young) and 9 months (adult) of age were fed on purified diets, supplemented with or without cholesterol, containing 200 g protein/kg (casein (CAS), whey protein (WHY) or soya-bean protein (SOY)) for 4 weeks.2. SOY exerted a hypocholesterolaemic effect in young rats regardless of dietary cholesterol, whereas in adult rats it was observed only when a cholesterol-enriched diet was given. WHY was also hypocholesterolaemic in rats of both ages given cholesterol. SOY tended to reduce the liver cholesterol both in young and adult rats.3. The liver hydroxymethylglutaryl-CoA reductase (NADPH) (EC 1.1.1.34) activity tended to be lower in the vegetable-protein groups than in the animal-protein groups in young rats, but the age-related reduction was observed only in the latter groups.4. There was no significant age-related difference in the activity of liver cholesterol 7α-monooxygenase (EC 1.14.13.17) in response to diets. However, when cholesterol was given, activity tended to decrease with age. Rats given SOY excreted more faecal steroids than those given casein, particularly adult rats.5. The fatty acid profile of phosphatidylcholine and the Δ6-desaturase activity of liver microsomes indicated the reduced desaturation of linoleate in the SOY and WHY groups compared with the CAS groups.6. The results thus showed a complex interaction of protein type, cholesterol and age on cholesterol homeostasis.

Gestational and early postnatal dietary NaCl levels affect NaCl intake, but not stimulated water intake, by adult rats

2004 ◽  
Vol 286 (6) ◽  
pp. R1043-R1050 ◽  
Author(s):  
Kathleen S. Curtis ◽  
Eric G. Krause ◽  
Donna L. Wong ◽  
Robert J. Contreras
Keyword(s):  
Water Intake ◽  
Body Fluid ◽  
Male Rats ◽  
Short Term ◽  
Adult Rats ◽  
Weanling Rats ◽  
Fluid Regulation ◽  
Ad Libitum ◽  
Concentrated Solution ◽  
Body Fluid Regulation

We examined body fluid regulation by weanling (21–25 days) and adult (>60 days) male rats that were offspring of dams fed chow containing either 0.1, 1, or 3% NaCl throughout gestation and lactation. Weanling rats were maintained on the test diets until postnatal day 30 and on standard 1% NaCl chow thereafter. Ad libitum water intake by weanlings was highest in those fed 3% NaCl and lowest in those fed 0.1% NaCl. Adult rats maintained on standard NaCl chow consumed similar amounts of water after overnight water deprivation or intravenous hypertonic NaCl (HS) infusion regardless of early NaCl condition. Moreover, baseline and HS-stimulated plasma Na+ concentrations also were similar for the three groups. Nonetheless, adult rats in the early 3% NaCl group consumed more of 0.5 M NaCl after 10 days of dietary Na+ deprivation than did rats in either the 1% or 0.1% NaCl group. Interestingly, whether NaCl was consumed in a concentrated solution in short-term, two-bottle tests after dietary Na+ deprivation or in chow during ad libitum feeding, adult rats in the 3% NaCl group drank less water for each unit of NaCl consumed, whereas rats in the 0.1% NaCl group drank more water for each unit of NaCl consumed. Thus gestational and early postnatal dietary NaCl levels do not affect stimulated water intake or long-term body fluid regulation. Together with our previous studies, these results suggest that persistent changes in NaCl intake and in water intake associated with NaCl ingestion reflect short-term behavioral effects that may be attributable to differences in NaCl taste processing.

ON RAT SERUM LIPASE

1952 ◽  
Vol 30 (1) ◽  
pp. 26-35
Author(s):  
Jules Tuba ◽  
Jack D. Taylor
Keyword(s):  
Food Consumption ◽  
Adult Male ◽  
Elevated Serum ◽  
Male Rats ◽  
Normal Male ◽  
Adult Rats ◽  
Rat Serum ◽  
Serum Lipase ◽  
Fat Consumption ◽  
Daily Food

There is no correlation in either weanling or adult normal male rats between daily food consumption of a stock laboratory diet and serum tributyrinase activity. Limiting the food intake of adult male rats has no statistically significant effects on serum tributyrinase. The abnormally elevated serum tributyrinase levels of alloxan diabetic adult male rats may be accounted for on the basis of increased food consumption. Weanling and adult male animals maintained on synthetic diets containing from 5 to 60% fat show a correlation between serum tributyrinase concentration and daily fat consumption. In the case of adult rats increased fat ingestion is accompanied by increased enzyme activity. However, with weanling rats increased fat consumption results in lowered tributyrinase concentrations and retarded growth. The results with growing animals are attributed to the decreased intake of food, and the corresponding decrease in protein consumption, which accompanies the increase in dietary fat concentration. Replacement of the synthetic diets by the stock laboratory diet is followed by altered tributyrinase levels in both weanling and adult animals.

The Response of Rat Serum Lipids to Diets of Varying Composition or Contaminated with Organochlorine Pesticides

1996 ◽  
Vol 51 (1-2) ◽  
pp. 91-100 ◽  
Author(s):  
Meinrad Boll ◽  
Lutz W. D. Weber ◽  
Andreas Stampfl
Keyword(s):  
Serum Lipids ◽  
Diet Composition ◽  
Considerable Increase ◽  
Serum Triglyceride ◽  
Dependent Manner ◽  
Lipid Levels ◽  
Serum Triglycerides ◽  
Rat Serum ◽  
Feeding Diets ◽  
Dose Dependent

Abstract The effects of different diets (high carbohydrate, high protein, high fat) and diets contaminated with polychlorinated biphenyls (PCBs) and/or γ-hexachlorocyclohexane (lindane) on the levels of serum triglycerides, cholesterol and phospholipids were investigated in Wistar rats. Serum triglyceride levels differed significantly among the diets, while those of cholesterol and phospholipids were much less affected by the diet composition. A change in diet composition resulted in a gradual adaptation to the lipid levels characteristic of the new diet with major variations including oscillations. There was, however, no specific component of a diet that could be associated with any specific change in serum lipids. While feed deprivation decreased the serum lipids (40-65% in 3 days), refeeding the starved animals caused pronounced increases of the lipids that were different among the diets. The response of the triglyceride levels was the strongest (up to 10 times the starvation levels) followed by those of the phospholipids (4-fold) and cholesterol (2.5-fold). Response of the triglyceride levels peaked within 1 or 2 days of refeeding, whereas those of cholesterol and phospholipids took 4 days to reach the maximum. Feeding PCB-contaminated diets increased the serum lipids in a dose-dependent manner (15-250 ppm). Higher PCB concentrations were increasingly inhibitory (350 ppm) or overtly toxic (> 400 ppm). Elevated lipids returned to the starting levels immediately after peaking (triglycerides) or only after several days (cholesterol, phospholipids) but with an earlier onset at lower PCB concentrations. Refeeding starved animals with PCB- contaminated diets also increased the serum lipids dose-dependently. Feeding lindane-containing diets (50-150 ppm) as well as refeeding animals with lindane diets resulted in a considerable increase of the triglyceride levels, while cholesterol and phospohlipids increased much less. Higher lindane concentrations ( 250 ppm) were inhibitory. The outcome on serum lipid levels on feeding diets contaminated with both PCBs and lindane was basically additive.

INDUCTION OF GOITRE BY PTU OR KClO4 IN MALE AND FEMALE RATS. EFFECT OF GONADECTOMY

1973 ◽  
Vol 74 (1) ◽  
pp. 88-104 ◽  
Author(s):  
T. Jolín ◽  
M. J. Tarin ◽  
M. D. Garcia
Keyword(s):  
Iodine Content ◽  
High Plasma ◽  
Disc Electrophoresis ◽  
Female Rats ◽  
Male Rats ◽  
Adult Rats ◽  
Male And Female ◽  
Glucose Levels ◽  
Male And Female Rats ◽  
Tsh Levels

ABSTRACT Male and female rats of varying ages were placad on a low iodine diet (LID) plus KClO4 or 6-propyl-2-thiouracil (PTU) or on the same diet supplemented with I (control rats). Goitrogenesis was also induced with LID plus PTU in gonadectomized animals of both sexes. The weight of the control and goitrogen treated animals, and the weight and iodine content of their thyroids were determined, as well as the plasma PBI, TSH, insulin and glucose levels. The pituitary GH-like protein content was assessed by disc electrophoresis on polyacrylamide gels. If goitrogenesis was induced in young rats of both sexes starting with rats of the same age, body weight (B.W.) and pituitary growth hormone (GH) content, it was found that both the males and females developed goitres of the same size. On the contrary, when goitrogenesis was induced in adult animals, it was found that male rats, that had larger B.W. and pituitary GH content than age-paired females, developed larger goitres. However, both male and female rats were in a hypothyroid condition of comparable degree as judged by the thyroidal iodine content and the plasma PBI and TSH levels. When all the data on the PTU or KClO4-treated male and female rats of varying age and B.W. were considered together, it was observed that the weights of the thyroids increased proportionally to B.W. However, a difference in the slope of the regression of the thyroid weight over B.W. was found between male and female rats, due to the fact that adult male rats develop larger goitres than female animals. In addition, in the male rats treated with PTU, gonadectomy decreased the B.W., pituitary content of GH-like protein and, concomitantly, the size of the goitre decreased; an opposite effect was induced by ovariectomy on the female animals. However, when goitrogenesis was induced in weight-paired adult rats of both sexes, the male animals still developed larger goitres than the females. Among all the parameters studied here, the only ones which appeared to bear a consistent relationship with the size of the goitres in rats of different sexes, treated with a given goitrogen, were the rate of body growth and the amount of a pituitary GH-like protein found before the onset of the goitrogen treatment. Moreover, though the pituitary content of the GH-like protein decreased as a consequence of goitrogen treatment, it was still somewhat higher in male that in female animals. The present results suggest that GH may somehow be involved in the mechanism by which male and female rats on goitrogens develop goitres of different sizes, despite equally high plasma TSH levels.

scholarly journals Stamina-Enhancing Effects of Human Adipose-Derived Stem Cells

2021 ◽  
Vol 30 ◽  
pp. 096368972110354
Author(s):  
Eun-Jung Yoon ◽  
Hye Rim Seong ◽  
Jangbeen Kyung ◽  
Dajeong Kim ◽  
Sangryong Park ◽  
...  
Keyword(s):  
Physical Activity ◽  
Stem Cells ◽  
Locomotor Activity ◽  
Tissue Injury ◽  
Male Rats ◽  
Adipose Derived Stem Cells ◽  
Sprague Dawley ◽  
Serum Triglycerides

Stamina-enhancing effects of human adipose derived stem cells (hADSCs) were investigated in young Sprague-Dawley rats. Ten-day-old male rats were transplanted intravenously (IV) or intracerebroventricularly (ICV) with hADSCs (1 × 106 cells/rat), and physical activity was measured by locomotor activity and rota-rod performance at post-natal day (PND) 14, 20, 30, and 40, as well as a forced swimming test at PND 41. hADSCs injection increased the moving time in locomotor activity, the latency in rota-rod performance, and the maximum swimming time. For the improvement of physical activity, ICV transplantation was superior to IV injection. In biochemical analyses, ICV transplantation of hADSCs markedly reduced serum creatine phosphokinase, lactate dehydrogenase, alanine transaminase, and muscular lipid peroxidation, the markers for muscular and hepatic injuries, despite the reduction in muscular glycogen and serum triglycerides as energy sources. Notably, hADSCs secreted brain-derived neurotrophic factor (BDNF) and nerve growth factor in vitro, and increased the level of BDNF in the brain and muscles in vivo. The results indicate that hADSCs enhance physical activity including stamina not only by attenuating tissue injury, but also by strengthening the muscles via production of BDNF.

scholarly journals Apolipoprotein A4-1/2 polymorphism and response of serum lipids to dietary cholesterol in humans

2000 ◽  
Vol 41 (10) ◽  
pp. 1623-1628
Author(s):  
Rianne M. Weggemans ◽  
Peter L. Zock ◽  
Saskia Meyboom ◽  
Harald Funke ◽  
Martijn B. Katan
Keyword(s):  
Serum Lipids ◽  
Dietary Cholesterol

scholarly journals Effects of Methanol Extract of Breadfruit (Artocarpus altilis) on Atherogenic Indices and Redox Status of Cellular System of Hypercholesterolemic Male Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Oluwatosin Adekunle Adaramoye ◽  
Olubukola Oyebimpe Akanni
Keyword(s):  
Methanol Extract ◽  
Dietary Cholesterol ◽  
Relative Weight ◽  
Redox Status ◽  
Cellular System ◽  
Male Rats ◽  
High Density Lipoproteins ◽  
Protective Effects ◽  
Artocarpus Altilis ◽  
Atherogenic Indices

We investigated the effects of methanol extract ofArtocarpus altilis(AA) on atherogenic indices and redox status of cellular system of rats fed with dietary cholesterol while Questran (QUE) served as standard. Biochemical indices such as total cholesterol (TC), triglycerides (TG), low- and high-density lipoproteins-cholesterol (LDL-C and HDL-C), aspartate and alanine aminotransferases (AST and ALT), lactate dehydrogenase (LDH), reduced glutathione, glutathione-s-transferase, glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), and lipid peroxidation (LPO) were assessed. Hypercholesterolemic (HC) rats had significantly increased relative weight of liver and heart. Dietary cholesterol caused a significant increase (P<0.05) in the levels of serum, hepatic, and cardiac TC by 110%, 70%, and 85%, LDL-C by 79%, 82%, and 176%, and TG by 68%, 96%, and 62%, respectively. Treatment with AA significantly reduced the relative weight of the organs and lipid parameters. There were beneficial increases in serum and cardiac HDL-C levels in HC rats treated with AA. In HC rats, serum LDH, ALT, and AST activities and levels of LPO were increased, whereas hepatic and cardiac SOD, CAT, and GPx were reduced. All biochemical and histological alterations were ameliorated upon treatment with AA. Extract of AA had protective effects against dietary cholesterol-induced hypercholesterolemia.

Gender-based differences in the effect of dietary cholesterol in rats

2012 ◽  
Vol 7 (6) ◽  
pp. 980-986 ◽  
Author(s):  
Milan Marounek ◽  
Zdeněk Volek ◽  
Eva Skřivanová ◽  
Marian Czauderna
Keyword(s):  
Dietary Cholesterol ◽  
Female Rats ◽  
Male Rats ◽  
Cholesterol Concentration ◽  
Hepatic Cholesterol ◽  
Bile Acid Concentration ◽  
Male And Female ◽  
Relative Expression ◽  
Male And Female Rats ◽  
Cholesterol Supplementation

AbstractMale and female rats were fed diets supplemented with cholesterol and palm fat at 10 and 50 g/kg, respectively; serum, hepatic tissue and faeces were analysed. Cholesterol supplementation significantly increased serum and hepatic cholesterol both in male and female rats. Male and female rats fed the cholesterol-containing diet differed significantly in serum cholesterol concentration (2.48 µmol/mL vs 2.92 µmol/mL), concentration of serum triacylglycerols, but not in hepatic cholesterol concentration. The serum and hepatic cholesterol concentrations correlated non-significantly in male rats (r=0.491; P=0.063) and significantly in female rats (r=0.818; P<0.001). Cholesterol supplementation non-significantly decreased relative expression of the hepatic LDL receptor gene and significantly increased relative expression of the hepatic cholesterol 7α-hydroxylase gene in rats of both genders. The faeces of control rats contained similar amounts of cholesterol and bile acids. Cholesterol supplementation increased cholesterol concentration 10 times in the faeces of male rats and 12 times in faeces of female rats. The corresponding increases of bile acid concentration were much lower (83% in male rats and 108% in female rats). It can be concluded that the effects of cholesterol supplementation were more pronounced in female than in male rats.

scholarly journals The effect of amino-acid-supplemented wheat gluten on cholesterol metabolism in the rat

1985 ◽  
Vol 53 (1) ◽  
pp. 25-30 ◽  
Author(s):  
M. Bassat ◽  
S. Mokady
Keyword(s):  
Amino Acid ◽  
Cholesterol Metabolism ◽  
Cholesterol Biosynthesis ◽  
Wheat Gluten ◽  
Blood Cholesterol ◽  
Amino Acid Supplementation ◽  
Faecal Excretion ◽  
Weanling Rats ◽  
Low Levels

1. The effect of lysine- and threonine-supplemented wheat gluten on cholesterol metabolism was studied using male weanling rats. Animals were fed on cholesterol-free diets containing 100 or 200 g gluten/kg with or without amino acid supplementation, and compared with animals given 50, 100 and 200 g casein/kg diets, for 3 weeks.2. A hypocholesterolaemic effect observed with the wheat gluten-fed rats, compared with the animals given 100 and 200 g casein/kg diets, was accompanied by increased turnover of cholesterol as expressed by enhanced cholesterol biosynthesis and increased faecal excretion of cholesterol and bile acids. This effect was not abolished by lysine and threonine supplementation.3. Low levels of blood cholesterol were also observed in the rats fed on the 50 g casein/kg diet. However, a different mechanism, related to impairment of cholesterol transport from the liver, was most likely responsible for the hypocholesterolaemia found in these protein-malnourished animals.4. The effect on cholesterol metabolism produced by dietary wheat gluten was independent of the low quality of the protein and of its specific deficiency in lysine and threonine.

Sign in / Sign up

Export Citation Format

Share Document