scholarly journals Determination of digesta flow entering the omasal canal of dairy cows using different marker systems

2003 ◽  
Vol 90 (1) ◽  
pp. 41-52 ◽  
Author(s):  
Seppo Ahvenjärvi ◽  
Aila Vanhatalo ◽  
Kevin J. Shingfield ◽  
Pekka Huhtanen
Keyword(s):  
Particulate Matter ◽  
Liquid Phase ◽  
Dairy Cows ◽  
High Speed ◽  
Volatile Fatty Acids ◽  
Accurate Determination ◽  
Water Soluble ◽  
Neutral Detergent Fibre ◽  
Inorganic Matter

Four studies were conducted to compare the effect of four indigestible markers (LiCoEDTA, Yb-acetate, Cr-mordanted straw and indigestible neutral-detergent fibre (INDF)) and three marker systems on the flow of digesta entering the omasal canal of lactating dairy cows. Samples of digesta aspirated from the omasal canal were pooled and separated using filtration and high-speed centrifugation into three fractions defined as the liquid phase, small particulate and large particulate matter. Co was primarily associated with the liquid phase, Yb was concentrated in small particulate matter, whilst Cr and INDF were associated with large particles. Digesta flow was calculated based on single markers or using the reconstitution system based on combinations of two (Co + Yb, Co + Cr and Co + INDF) or three markers (Co + Yb + Cr and Co + Yb + INDF). Use of single markers resulted in large differences between estimates of organic matter (OM) flow entering the omasal canal suggesting that samples were not representative of true digesta. Digesta appeared to consist of at least three phases that tended to separate during sampling. OM was concentrated in particulate matter, whilst the liquid phase consisted mainly of volatile fatty acids and inorganic matter. Yb was intimately associated with nitrogenous compounds, whereas Cr and INDF were concentrated in fibrous material. Current data indicated that marker systems based on Yb in combination with Cr or INDF are required for the accurate determination of OM, N and neutral-detergent fibre flow. In cases where the flow of water-soluble nutrients entering the omasal canal is also required, the marker system should also include Co.

Determination of leucocyte concentrations in cow's milk with a Coulter counter

1966 ◽  
Vol 33 (1) ◽  
pp. 51-64 ◽  
Author(s):  
L. W. Phipps ◽  
F. H. S. Newbould
Keyword(s):  
Particulate Matter ◽  
Accurate Determination ◽  
Total Cell ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Fat Globules ◽  
Cell Counts ◽  
Number Of Cells ◽  
Comparable Size

SummaryA technique for determining the concentration of leucocytes in cow's milk is described which is more rapid and accurate as well as much less tedious than conventional microscope methods. The leucocytes are isolated from the fat globules of comparable size by a novel centrifuging procedure. Sizing of the leucocytes with a Coulter electronic counter shows that their volumes range from about 45 to 1770 µm3but a partially overlapping distribution of other particulate matter prevents the accurate determination of the total number of cells present. The numbers counted in truncated leucocyte distributions, however, correlate linearly with total cell counts determined with an improved microscope method up to 6 × 106cells/ml of milk.

Factors affecting the accurate determination of cerebrovascular blood flow using high-speed droplet imaging

1998 ◽  
Author(s):  
Stephen Rudin ◽  
Afshin Divani ◽  
Ajay K. Wakhloo ◽  
Baruch B. Lieber ◽  
William Granger ◽  
...  
Keyword(s):  
Blood Flow ◽  
High Speed ◽  
Accurate Determination ◽  
Factors Affecting

scholarly journals Determination of the Optimum Removal Efficiency of Fine Particulate Matter Using Activated Carbon Fiber (ACF)

2020 ◽  
Vol 17 (21) ◽  
pp. 8230
Author(s):  
Min-Kyeong Kim ◽  
Yelim Jang ◽  
Duckshin Park
Keyword(s):  
Activated Carbon ◽  
Particulate Matter ◽  
Carbon Fiber ◽  
Removal Efficiency ◽  
High Speed ◽  
Fine Particulate Matter ◽  
Activated Carbon Fiber ◽  
Optimal Conditions ◽  
Before And After

In Korea, concentrations of particulate matter (PM10) are significantly higher in urban railway tunnels (178.1 μg/m3) than in metropolitan areas (49 μg/m3). In railway tunnels in Korea, it was maintained at 3–4 times higher concentration than general atmosphere and platform. Dust generated by trains is scattered at high speed in these tunnels, making filtration difficult; therefore, the development of filters that can be maintained in tunnels is required. In the present study, we examined PM adsorption in the laboratory scale using activated carbon fiber (ACF), which has high adsorption and capacity. The ACF depth, velocity of flow, and fine PM concentration in the tunnel were the experimental variables. We compared PM concentrations before and after the filter experiments, and calculated removal efficiency to determine the optimal conditions. Comprehensive examination of the experimental variables and differential pressure showed that the optimal conditions for an ACF specimen were a wind speed of 3.0 m/s and the ACF depth of 400 mm. The average removal efficiency of PM10 was 55.5%, and that of PM2.5 was 36.6%. The reproducibility tests showed that the ACF filter could be washed and reused and is suitable for various places because it is easily maintained.

Accurate Determination of the Cold Detection Threshold with High-Speed Cooling of the Skin

Pain Medicine ◽  
2020 ◽  
Vol 21 (12) ◽  
pp. 3428-3436
Author(s):  
Segolene Lithfous ◽  
Olivier Després ◽  
Thierry Pebayle ◽  
Claudia Casadio ◽  
Andre Dufour
Keyword(s):  
High Speed ◽  
Accurate Determination ◽  
Detection Threshold ◽  
Detection Thresholds ◽  
Temperature Ramp ◽  
Temperature Ramps ◽  
The Subject ◽  
Order Four ◽  
Healthy Participants

Abstract Objective This study used high-speed cooling of the skin and exact control of stimulus duration to measure the cold detection threshold in healthy participants. The objective was to compare the method of limits, in which the temperature is slowly and gradually increased/decreased until the subject perceives the stimulation, and the method of levels, in which the subject must detect brief thermal stimulations close to the threshold of perception. Methods Twenty healthy volunteers (nine women, 11 men) aged 20–30 years participated in the study. The method of limits and method of levels were performed in all subjects in a counterbalanced order. Four cold detection thresholds were measured with the method of levels, with a temperature ramp of 300°C/sec and stimulus durations of 50 ms, 100 ms, 300 ms, and 500 ms. Three thresholds were measured with the method of limits, with temperature ramps of 1°C/sec, 2°C/sec, and 4°C/sec. Results On average, the cold detection thresholds were −0.47°C below skin temperature with the method of levels and −1.67°C the method of limits. Interindividual variability was significantly lower with the method of levels than with the method of limits. Conclusions These results suggest that the method of levels is more accurate than the method of limits for measuring cold detection threshold. The improvement of cold detection threshold measurement may provide new perspectives to more precisely assess the function of A-delta fibers and the spino-thalamic pathway.

Sign in / Sign up

Export Citation Format

Share Document