scholarly journals Preliminary study on genetic relationships of Melocactus Link & Otto of the family Cactaceae revealed through karyotype, DNA content and RAPD analysis

Caryologia ◽  
2008 ◽  
Vol 61 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Das Anath Bandhu ◽  
Suprava Mohanty
Keyword(s):  
Dna Content ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
The Family ◽  
Preliminary Study

scholarly journals De Novo SNP Discovery and Genotyping of Iranian Pimpinella Species Using ddRAD Sequencing

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1342
Author(s):  
Shaghayegh Mehravi ◽  
Gholam Ali Ranjbar ◽  
Ghader Mirzaghaderi ◽  
Anita Alice Severn-Ellis ◽  
Armin Scheben ◽  
...  
Keyword(s):  
De Novo ◽  
Genetic Relationships ◽  
Nucleotide Polymorphisms ◽  
High Quality ◽  
Genomic Resources ◽  
High Quality Snps ◽  
The Family ◽  
Double Digestion ◽  
Flanking Sequences ◽  
Downstream Analysis

The species of Pimpinella, one of the largest genera of the family Apiaceae, are traditionally cultivated for medicinal purposes. In this study, high-throughput double digest restriction-site associated DNA sequencing technology (ddRAD-seq) was used to identify single nucleotide polymorphisms (SNPs) in eight Pimpinella species from Iran. After double-digestion with the enzymes HpyCH4IV and HinfI, a total of 334,702,966 paired-end reads were de novo assembled into 1,270,791 loci with an average of 28.8 reads per locus. After stringent filtering, 2440 high-quality SNPs were identified for downstream analysis. Analysis of genetic relationships and population structure, based on these retained SNPs, indicated the presence of three major groups. Gene ontology and pathway analysis were determined by using comparison SNP-associated flanking sequences with a public non-redundant database. Due to the lack of genomic resources in this genus, our present study is the first report to provide high-quality SNPs in Pimpinella based on a de novo analysis pipeline using ddRAD-seq. This data will enhance the molecular knowledge of the genus Pimpinella and will provide an important source of information for breeders and the research community to enhance breeding programs and support the management of Pimpinella genomic resources.

Phylogenetic relationships of five morphological groups of hexaploid wheat (Triticum aestivum L. em Thell.) based on RAPD analysis

Genome ◽  
2000 ◽  
Vol 43 (4) ◽  
pp. 724-727 ◽  
Author(s):  
Wenguang Cao ◽  
G Scoles ◽  
P Hucl ◽  
R N Chibbar
Keyword(s):  
Hexaploid Wheat ◽  
Phylogenetic Relationships ◽  
Genetic Similarity ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Triticum Aestivum L ◽  
Similarity Coefficients ◽  
Morphological Classification ◽  
Wild Wheat

The genetic relationships among the five groups of hexaploid wheat: common, spelta, macha, vavilovii, and semi-wild wheat (SWW) are not clear. Random amplified polymorphic DNA (RAPD) analysis was used to assess phylogenetic relationships among these five morphological groups of hexaploid wheat. RAPD data were analyzed using the NTSYS-PC computer program to generate Jaccard genetic similarity coefficients. A dendrogram based on RAPD analysis grouped 15 accessions into five distinct clusters. These results are in agreement with those based on morphological classification, suggesting that common wheat is most closely related to SWW, followed by spelta, vavilovii, and macha.Key words: RAPD, macha, spelta, vavilovii, semi-wild wheat, phylogenetic relationships.

scholarly journals Galls of Cecidoses eremita Curtis and Eucecidoses minutanus Brèthes (Lepidoptera: Cecidosidae) in Magdalena, Buenos Aires Province: preliminary study and associated fauna

2016 ◽  
Vol 16 (4) ◽  
Author(s):  
Veronica Loetti ◽  
Alejandra Valverde ◽  
Diana Nora Rubel
Keyword(s):  
Wall Thickness ◽  
Buenos Aires ◽  
Maximum Diameter ◽  
Buenos Aires Province ◽  
Associated Fauna ◽  
The Family ◽  
Preliminary Study

Abstract In Argentina, five galling species of the family Cecidosidae (Lepidoptera), including Cecidoses eremita Curtis and Eucecidoses minutanus Brèthes, have been cited. This note reports a preliminary study of their galls in "molles" (Schinus longifolius (Lindl.) Speg.) of Magdalena (Buenos Aires, Argentina). In April 2013, galls of C. eremita and E. minutanus were censused in randomly selected "molles". In April and December 2013, randomly selected galls were then collected and transported to our laboratory in hermetic bags, and conserved at -18 ºC until examination. Maximum diameter and wall thickness of collected galls were measured. About 84% of the observed "molles" (103/123) had galls of C. eremita and/or E. minutanus. The median of galls per tree was 12 (Q1=6; Q3=22). Eucecidoses minutanus had galls with smaller diameter (U11;53= 583; P<0.05) and thinner wall (U10;52=506.5; P<0..05) than C. eremita. In open galls, we found Pseudoescorpionida, Araneae (Segestriidae and Salticidae), and larvae of Lepidoptera and Hymenoptera. Within closed galls, we found adults of Torymidae and Chalcidoidea, and larvae of Ichneumonoidea.

Cytogenetic and DNA content in six genera of the family Callichthyidae (Pisces, Siluriformes)

Caryologia ◽  
1993 ◽  
Vol 46 (2-3) ◽  
pp. 171-188 ◽  
Author(s):  
Claudio Oliveira ◽  
Lurdes F. Almeida-Toledo ◽  
Lyria Mori ◽  
Silvio Almeida Toledo-Filho
Keyword(s):  
Dna Content ◽  
The Family

DNA content of cells in human buccal smears A preliminary study

1978 ◽  
Vol 62 (2) ◽  
pp. 276-281 ◽  
Author(s):  
Jan Rowiński ◽  
Catherine Souchier ◽  
Jean-Claude Czyba
Keyword(s):  
Dna Content ◽  
Preliminary Study

scholarly journals Preliminary study of the enzyme ubiquitin carboxylterminal hydrolase 14 (UBP6) in Giardia intestinalis: structural bioinformatic analysis and transcriptional profile during encystation

2015 ◽  
Vol 43 (2) ◽  
pp. 32-40
Author(s):  
Magda E Alvarado ◽  
Camila A González ◽  
Moisés Wasserman ◽  
Claudia C Rubiano
Keyword(s):  
Protein Turnover ◽  
Bioinformatic Analysis ◽  
Giardia Intestinalis ◽  
Deubiquitinating Enzyme ◽  
Combined Approach ◽  
Protein Coding ◽  
Starting Point ◽  
The Family ◽  
Preliminary Study ◽  
Carboxyl Terminal

This paper presents a combined approach<br />with two aims. The first is to analyze the<br />reported sequence of the enzyme ubiquitin<br />carboxyl-terminal hydrolase 14 of Giardia<br />intestinalis (UBP6) through computational<br />methods to find components related with<br />its hypothetical function. The second is<br />to determine if the protein-coding gene is<br />expressed in G. intestinalis and, if such is<br />the case, also determine its transcription<br />pattern along the life cycle of the parasite. It<br />was established that the protein belongs to<br />the family of Cys-dependent deubiquitinases<br />and more specifically to ubiquitin specific<br />proteases (USPs). Moreover, the catalytic<br />center with the complete triad as well as<br />typical features of the USP motif were also<br />identified. Since the computational findings<br />suggest that the enzyme could be functional,<br />reverse transcription coupled to PCR was<br />used as a first approach to establish if in fact<br />the coding gene is expressed in the parasite.<br />Interestingly, it was found not only that<br />the gene is expressed, but also that there<br />is a transcription variation along the life<br />cycle of the parasite. These two findings are<br />the starting point for further studies since<br />they tentatively suggest that this enzyme<br />could be involved in the protein turnover<br />that occurs during parasite encystation.<br />Although preliminary, this study is the first<br />report concerning the study of a specific<br />deubiquitinating enzyme in the parasite G.<br />intestinalis.

Linguistic Relativity in Fiji: A Preliminary Study

1997 ◽  
Vol 9 ◽  
pp. 69-74 ◽  
Author(s):  
Robin Taylor
Keyword(s):  
Extended Family ◽  
The Other ◽  
Relativity Theory ◽  
Linguistic Relativity ◽  
Extended Study ◽  
Experimental Support ◽  
The Family ◽  
Preliminary Study ◽  
Group Reading ◽  
The Individual

AbstractThere is a lack of experimental support for Linguistic Relativity Theory (LRT), which has not been tested in a South Pacific context. Fifty-two bilingual male (n = 26) and female Fijians read, and answered survey questions on the family dilemma, “An Unwanted Child?” - one group functioning in English and the other in Fijian. The group reading and answering in Fijian tended to place more emphasis on the rights of the extended family, whereas the group reading and responding in English placed more emphasis on the rights of the individual. These preliminary findings are consistent with LRT theory, and form the basis for more extended study, including perhaps a wider range of dilemmas and linguistic abilities (e.g., Fijians living in Australia).

Assessment of genetic relationships among rhododendron species, varieties and hybrids by RAPD analysis

1995 ◽  
Vol 63 (3-4) ◽  
pp. 215-223 ◽  
Author(s):  
M.Javed Iqbal ◽  
D.W. Paden ◽  
A.Lane Rayburn
Keyword(s):  
Rapd Analysis ◽  
Genetic Relationships
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