Physiological status and microbial diversity assessment of microbial mats: The signature lipid biomarker approach

Ophelia ◽  
2004 ◽  
Vol 58 (3) ◽  
pp. 165-173 ◽  
Author(s):  
Laura Villanueva ◽  
Antoni Navarrete ◽  
Jordi Urmeneta ◽  
David C. White ◽  
Ricardo Guerrero
Keyword(s):  
Microbial Diversity ◽  
Microbial Mats ◽  
Physiological Status ◽  
Lipid Biomarker ◽  
Diversity Assessment

scholarly journals Cultivation and Genomic, Nutritional, and Lipid Biomarker Characterization of Roseiflexus Strains Closely Related to Predominant In Situ Populations Inhabiting Yellowstone Hot Spring Microbial Mats

2010 ◽  
Vol 192 (12) ◽  
pp. 3033-3042 ◽  
Author(s):  
Marcel T. J. van der Meer ◽  
Christian G. Klatt ◽  
Jason Wood ◽  
Donald A. Bryant ◽  
Mary M. Bateson ◽  
...  
Keyword(s):  
National Park ◽  
Microbial Mats ◽  
Hot Spring ◽  
Small Subunit ◽  
Small Subunit Rrna ◽  
Anoxygenic Phototrophs ◽  
Lipid Biomarker ◽  
Genes Encoding

ABSTRACT Roseiflexus sp. strains were cultivated from a microbial mat of an alkaline siliceous hot spring in Yellowstone National Park. These strains are closely related to predominant filamentous anoxygenic phototrophs found in the mat, as judged by the similarity of small-subunit rRNA, lipid distributions, and genomic and metagenomic sequences. Like a Japanese isolate, R. castenholzii, the Yellowstone isolates contain bacteriochlorophyll a, but not bacteriochlorophyll c or chlorosomes, and grow photoheterotrophically or chemoheterotrophically under dark aerobic conditions. The genome of one isolate, Roseiflexus sp. strain RS1, contains genes necessary to support these metabolisms. This genome also contains genes encoding the 3-hydroxypropionate pathway for CO2 fixation and a hydrogenase, which might enable photoautotrophic metabolism, even though neither isolate could be grown photoautotrophically with H2 or H2S as a possible electron donor. The isolates exhibit temperature, pH, and sulfide preferences typical of their habitat. Lipids produced by these isolates matched much better with mat lipids than do lipids produced by R. castenholzii or Chloroflexus isolates.

Monitoring deep subsurface microbiota for assessment of safe long-term nuclear waste disposal

1996 ◽  
Vol 42 (4) ◽  
pp. 375-381 ◽  
Author(s):  
David C. White ◽  
David B. Ringelberg
Keyword(s):  
Nuclear Waste ◽  
Waste Disposal ◽  
Nuclear Waste Disposal ◽  
Physiological Status ◽  
Deep Subsurface ◽  
Long Term Storage ◽  
Lipid Biomarker ◽  
Potential Problems

Microbes with their resistance to heat and radioactivity, if present and metabolically active, could have major effects on the safety of nuclear waste disposal by posing potential problems in long-term containment. This paper reviews the applicability of the signature lipid biomarker (SLB) analysis in the quantitative assessment of the viable biomass, community composition, and nutritional/physiological status of the subsurface microbiota as it exists in situ in subsurface samples. The samples described in this review are not unlike those expected to be recovered from proposed deep subsurface disposal sites. Assessment of the microbial community ecology using SLB analysis can be utilized to predict potential problems engendered by microbial metabolic activities of these communities in breaching containment by microbially facilitated corrosion and in the potential for subsequent facilitated transport of nuclides into the environment. SLB analysis of the in situ microbial ecology can be utilized to monitor the feasibility of containment options in modeling tests at the specific disposal sites.Key words: nuclear waste, deep subsurface, microbiota, microbial corrosion, safe long-term storage, signature lipid biomarkers.

scholarly journals Combined Phospholipid Biomarker-16S rRNA Gene Denaturing Gradient Gel Electrophoresis Analysis of Bacterial Diversity and Physiological Status in an Intertidal Microbial Mat

2004 ◽  
Vol 70 (11) ◽  
pp. 6920-6926 ◽  
Author(s):  
Laura Villanueva ◽  
Antoni Navarrete ◽  
Jordi Urmeneta ◽  
David C. White ◽  
Ricardo Guerrero
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Microbial Mat ◽  
Physiological Status ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Lipid Biomarker ◽  
Gradient Gel Electrophoresis

ABSTRACT A combined lipid biomarker-16S rRNA gene denaturing gradient gel electrophoresis analysis was used to monitor changes in the physiological status, biomass, and microbial composition of a microbial mat. In the morning hours, an increase in the biomass of layers containing a high density of phototrophs and a decrease in the growth rate in the deep layers were observed. The combined approach also revealed differences in major groups of microorganisms, including green nonsulfur, gram-positive, and heterotrophic bacteria.

Lipid biomarker analysis of cyanobacteria-dominated microbial mats in meltwater ponds on the McMurdo Ice Shelf, Antarctica

2009 ◽  
Vol 40 (2) ◽  
pp. 258-269 ◽  
Author(s):  
Anne D. Jungblut ◽  
Michelle A. Allen ◽  
Brendan P. Burns ◽  
Brett A. Neilan
Keyword(s):  
Microbial Mats ◽  
Ice Shelf ◽  
Lipid Biomarker ◽  
Biomarker Analysis

scholarly journals Shotgun metagenomic sequencing based microbial diversity assessment of Lasundra hot spring, India

Genomics Data ◽  
2015 ◽  
Vol 4 ◽  
pp. 73-75 ◽  
Author(s):  
Amit V. Mangrola ◽  
Pravin Dudhagara ◽  
Prakash Koringa ◽  
C.G. Joshi ◽  
Rajesh K. Patel
Keyword(s):  
Microbial Diversity ◽  
Hot Spring ◽  
Metagenomic Sequencing ◽  
Shotgun Metagenomic Sequencing ◽  
Diversity Assessment

scholarly journals Effects of Elevated Carbon Dioxide and Salinity on the Microbial Diversity in Lithifying Microbial Mats

Minerals ◽  
2014 ◽  
Vol 4 (1) ◽  
pp. 145-169 ◽  
Author(s):  
Steven Ahrendt ◽  
Jennifer Mobberley ◽  
Pieter Visscher ◽  
Lawrence Koss ◽  
Jamie Foster
Keyword(s):  
Carbon Dioxide ◽  
Microbial Diversity ◽  
Microbial Mats ◽  
Elevated Carbon Dioxide
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