A Novel Approach to Enhance the Urease Activity of Sporosarcina pasteurii and its Application on Microbial-Induced Calcium Carbonate Precipitation for Sand

2019 ◽  
Vol 36 (9) ◽  
pp. 819-825
Author(s):  
Yang Zhao ◽  
Zhiyang Xiao ◽  
Jie Lv ◽  
Wanqing Shen ◽  
Rongchao Xu
Crystals ◽  
2018 ◽  
Vol 8 (5) ◽  
pp. 189 ◽  
Author(s):  
Chun-Mei Hsu ◽  
Yi-Hsun Huang ◽  
Vanita Nimje ◽  
Wen-Chien Lee ◽  
How-Ji Chen ◽  
...  

The Analyst ◽  
2016 ◽  
Vol 141 (10) ◽  
pp. 2887-2895 ◽  
Author(s):  
Dustin Harris ◽  
Jyothir Ganesh Ummadi ◽  
Andrew R. Thurber ◽  
Yvan Allau ◽  
Circe Verba ◽  
...  

Chemical and morphological mapping of live bacterial assisted calcium carbonate precipitation using scanning electrochemical microscope (SECM).


2020 ◽  
Vol 10 (13) ◽  
pp. 4538 ◽  
Author(s):  
Johannes Hommel ◽  
Arda Akyel ◽  
Zachary Frieling ◽  
Adrienne J. Phillips ◽  
Robin Gerlach ◽  
...  

Enzymatically induced calcium carbonate precipitation (EICP) is an emerging engineered mineralization method similar to others such as microbially induced calcium carbonate precipitation (MICP). EICP is advantageous compared to MICP as the enzyme is still active at conditions where microbes, e.g., Sporosarcina pasteurii, commonly used for MICP, cannot grow. Especially, EICP expands the applicability of ureolysis-induced calcium carbonate mineral precipitation to higher temperatures, enabling its use in leakage mitigation deeper in the subsurface than previously thought to be possible with MICP. A new conceptual and numerical model for EICP is presented. The model was calibrated and validated using quasi-1D column experiments designed to provide the necessary data for model calibration and can now be used to assess the potential of EICP applications for leakage mitigation and other subsurface modifications.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Raja Murugan ◽  
G. K. Suraishkumar ◽  
Abhijit Mukherjee ◽  
Navdeep K. Dhami

AbstractMicrobially induced calcium carbonate precipitation (MICP)/Biocementation has emerged as a promising technique for soil engineering applications. There are chiefly two methods by which MICP is applied for field applications including biostimulation and bioaugmentation. Although bioaugmentation strategy using efficient ureolytic biocementing culture of Sporosarcina pasteurii is widely practiced, the impact of native ureolytic microbial communities (NUMC) on CaCO3 mineralisation via S. pasteurii has not been explored. In this paper, we investigated the effect of different concentrations of NUMC on MICP kinetics and biomineral properties in the presence and absence of S. pasteurii. Kinetic analysis showed that the biocementation potential of S. pasteurii is sixfold higher than NUMC and is not significantly impacted even when the concentration of the NUMC is eight times higher. Micrographic results revealed a quick rate of CaCO3 precipitation by S. pasteurii leading to generation of smaller CaCO3 crystals (5–40 µm), while slow rate of CaCO3 precipitation by NUMC led to creation of larger CaCO3 crystals (35–100 µm). Mineralogical results showed the predominance of calcite phase in both sets. The outcome of current study is crucial for tailor-made applications of MICP.


2021 ◽  
Author(s):  
Raja Murugan ◽  
G. K. Suraishkumar ◽  
Abhijit Muhkerjee ◽  
Navdeep K Dhami

Abstract Microbially induced calcium carbonate precipitation (MICP)/Biocementation has emerged as a promising technique for soil engineering applications. There are chiefly two methods by which MICP is applied for field applications including biostimulation and bioaugmentation. Although bioaugmentation strategy using efficient ureolytic biocementing culture of Sporosarcina pasteurii is widely practiced, the impact of native ureolytic microbial communities (NUMC) on CaCO3 mineralisation via S. pasteurii has not been explored. In this paper, we investigated the effect of different concentrations of NUMC on MICP kinetics and biomineral properties in the presence and absence of S. pasteurii. Kinetic analysis showed that the biocementation potential of S. pasteurii is 6-fold higher than the NUMC and is not significantly impacted even when the concentration of the NUMC is eight times higher. Micrographic results revealed a quick rate of CaCO3 precipitation by S. pasteurii led to the generation of smaller CaCO3 crystals (5–40 µm), while the slow rate of CaCO3 precipitation by NUMC led to the creation of larger CaCO3 crystals (35–100 µm). Mineralogical results showed the predominance of the calcite phase in both sets. The outcome of the current study is crucial for tailor-made applications of MICP.


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