Enzyme-assisted extraction of cordycepin and adenosine from cultured Cordyceps militaris and purification by macroporous resin column chromatography

For the full development and utilization of Sophora tonkinensis Gagnep., this study was primarily intended to established a simple and efficient approach for the preparative purification of total flavonoids from S. tonkinensis by macroporous resin column chromatography (MRCC). The adsorption and desorption characteristics of the total flavonoids on ten macroporous resins were first studied, and AB-8 resin was chosen as the most suitable, and the adsorption data were best fitted to the pseudo-second-order kinetics model and Langmuir isotherm model. Furthermore, the technological parameters for the purification of the total flavonoids were optimized using column chromatography. After a sample one-step purification procedure, the content of the total flavonoids increased by about 4.76-fold from 12.14% to 57.82%, with a recovery yield of 84.93%. In addition, the comparative analysis of the flavonoid extracts before and after purification was performed by high-performance liquid chromatography coupled with photodiode-array detection (HPLC-PAD). The results showed that the contents of six major flavonoids in the purified product were all higher than before the purification. Therefore, the AB-8 MRCC established in this work was a promising method for the industrial-scale purification of the total flavonoids from S. tonkinensis.

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Optimization of Enzyme-Assisted Extraction of Carotenoids Antioxidants from Cordyceps militaris Using Response Surface Methodology

International Journal of Food Engineering ◽

10.1515/ijfe-2016-0173 ◽

2017 ◽

Vol 13 (5) ◽

Cited By ~ 2

Author(s):

Bai-Juan Wang ◽

Qing-Song Yang ◽

Ting Chen ◽

Xiang-Dong Qin ◽

Jun-Rong Ma ◽

...

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Cordyceps Militaris ◽

Extraction Temperature ◽

Scavenging Activity ◽

Assisted Extraction ◽

Development And Utilization ◽

Enzyme Assisted Extraction

Abstract Response surface methodology (RSM) with a Box–Behnken design (BBD) was used to optimize the extraction conditions of carotenoid antioxidants from Cordyceps militaris. An enzyme-assisted extraction (EAE) method was developed and optimized to enhance 2,2-diphenyl-1-picrylhydrazil (DPPH) radical-scavenging activity of carotenoid antioxidants from C. militaris. The optimum conditions were as follows: a pH of 4.1, an extraction time of 40.4 min, an extraction temperature of 48.7 °C and 0.43 % enzyme (cellulose: pectinase, 1:1, w/w) concentration. Under these conditions, the DDPH radical-scavenging activity of C. militaris extracts was 81.62±2.26 %, indicating that the BBD is an efficient approach to develop mathematical models for predictingcarotenoids antioxidant extraction from C. militaris. The successful extraction of carotenoid antioxidants from C. militaris provides a basis for the development and utilization of C. militaris resources.

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