Optimum Conditions in Preparative Liquid Chromatography. II. Selection of Column Dimensions

1987 ◽  
Vol 22 (8-10) ◽  
pp. 1869-1880 ◽  
Author(s):  
Klaus-Peter Hupe ◽  
Bernd Hoffmann
Keyword(s):  
Liquid Chromatography ◽  
Optimum Conditions ◽  
Preparative Liquid Chromatography ◽  
Selection Of

scholarly journals DEVELOPMENT OF THE TECHNOLOGY FOR THE SEPARATION OF THE COMPONENTS FROM GREEN TEA USING THE PREPARATIVE LIQUID CHROMATOGRAPHY BY DESIGN OF PERIODICAL PROCESS

2021 ◽  
pp. 327-336
Author(s):  
Elena Petrovna Muelchi ◽  
Marina Viktorovna Andryukhova
Keyword(s):  
Liquid Chromatography ◽  
Green Tea ◽  
Epigallocatechin Gallate ◽  
Tea Plant ◽  
High Yield ◽  
Preparative Liquid Chromatography ◽  
Anticancer Effects ◽  
Commercial Process ◽  
Tea Extract ◽  
Selection Of

Leaves of the green tea plant camellis sinensis contain up to 36 % polyphenols. Epigallocatechin gallate (EGCG) is the most interesting polyphenols because it exhibits a strong antioxidant effect. Furthermore, it has been demonstrated that EGCG has an antimutagenic and an anticancer effects, an antibacterial effect and a beneficial effect on cholesterol level in blood. Therefore, is needed to isolate EGCG in a pure form in high yield by simple and commercial process. This method for isolation of EGCG contents the following steps: a) selection of macroporous polar resin for the preparative liquid chromatography; b) selection of polar elution solvent; c) determination the temperature and pressure for adsorbtion and desorbtion of the polyphenols of the green tea. Therefore, it was searched the interconnection between yield of EGCG and the range of temperature and amount of eluent. Finally, the process for the separation of Epigallocatechin gallate from green tea extract was developed in the laboratory and EGCG was isolated with the concentration 92% by the yield 43%. Accordingly, the technology of EGCG production based on the preparative liquid chromatography was launched and introduced on the market.

Separation and Determination of Some Aromatic Sulfones by Reversed-Phase High-Performance Liquid Chromatography

1994 ◽  
Vol 59 (3) ◽  
pp. 569-574 ◽  
Author(s):  
Josef Královský ◽  
Marta Kalhousová ◽  
Petr Šlosar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Uv Spectra ◽  
Optimum Conditions ◽  
Linear Calibration ◽  
Technical Grade

The reversed-phase high-performance liquid chromatography of some selected, industrially important aromatic sulfones has been investigated. The chromatographic behaviour of three groups of aromatic sulfones has been studied. The optimum conditions of separation and UV spectra of the sulfones and some of their hydroxy and benzyloxy derivatives are presented. The dependences of capacity factors vs methanol content in mobile phase are mentioned. The results obtained have been applied to the quantitative analysis of different technical-grade samples and isomer mixtures. For all the separation methods mentioned the concentration ranges of linear calibration curves have been determined.

scholarly journals Deep Q-learning for the selection of optimal isocratic scouting runs in liquid chromatography

2021 ◽  
Vol 1638 ◽  
pp. 461900
Author(s):  
Alexander Kensert ◽  
Gilles Collaerts ◽  
Kyriakos Efthymiadis ◽  
Gert Desmet ◽  
Deirdre Cabooter
Keyword(s):  
Liquid Chromatography ◽  
Q Learning ◽  
Selection Of

Multidimensional preparative liquid chromatography to isolate flavonoids from bergamot juice and evaluation of their anti-inflammatory potential

2015 ◽  
Vol 38 (24) ◽  
pp. 4196-4203 ◽  
Author(s):  
Marina Russo ◽  
Paola Dugo ◽  
Stefania Marzocco ◽  
Veronica Inferrera ◽  
Luigi Mondello
Keyword(s):  
Liquid Chromatography ◽  
Preparative Liquid Chromatography ◽  
Anti Inflammatory
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