Origin, spreading and genetic variability of chicken anemia virus

2021 ◽  
pp. 1-37
Author(s):  
Claudia Techera ◽  
Ana Marandino ◽  
Gonzalo Tomás ◽  
Sofía Grecco ◽  
Martín Hernández ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Chicken Anemia Virus ◽  
Anemia Virus

scholarly journals Performance comparison between broilers positive and negative for antibodies against the chicken anemia virus

2003 ◽  
Vol 34 ◽  
pp. 88-89
Author(s):  
Lauricio Librelotto Rubin ◽  
Luiz Antônio Faccenda de Ávila ◽  
Andréa Machado Leal Ribeiro ◽  
Vera Wald ◽  
Cláudio Wageck Canal
Keyword(s):  
Performance Comparison ◽  
Chicken Anemia Virus ◽  
Anemia Virus

scholarly journals Positive and Negative Regulation of Chicken Anemia Virus Transcription

2005 ◽  
Vol 79 (5) ◽  
pp. 2859-2868 ◽  
Author(s):  
Myrna M. Miller ◽  
Keith W. Jarosinski ◽  
Karel A. Schat
Keyword(s):  
Estrogen Receptor ◽  
Nuclear Receptor ◽  
Protein Translation ◽  
Chicken Anemia Virus ◽  
Egfp Expression ◽  
Start Site ◽  
Response Element ◽  
Nuclear Receptor Superfamily ◽  
Promoter Construct ◽  
Anemia Virus

ABSTRACT Chicken anemia virus (CAV) is a small circular single-stranded DNA virus with a single promoter-enhancer region containing four consensus cyclic AMP response element sequences (AGCTCA), which are similar to the estrogen response element (ERE) consensus half-sites (A)GGTCA. These sequences are arranged as direct repeats, an arrangement that can be recognized by members of the nuclear receptor superfamily. Transient-transfection assays which use a short CAV promoter construct that ended at the transcription start site and drive expression of enhanced green fluorescent protein (EGFP) showed high basal activity in DF-1, LMH, LMH/2A, and primary theca and granulosa cells. The estrogen receptor-enhanced cell line, LMH/2A, had significantly greater expression than LMH cells, and this expression was significantly increased with estrogen treatment. A long promoter construct which included GGTCA-like sequences downstream of the first CAV protein translation start site was found to have significantly less EGFP expression in DF-1 cells than the short promoter, which was largely due to decreased RNA transcription. DNA-protein binding assays indicated that proteins recognizing a consensus ERE palindrome also bind GGTCA-like sequences in the CAV promoter. Estrogen receptor and other members of the nuclear receptor superfamily may provide a mechanism to regulate CAV activity in situations of low virus copy number.

Genome sequencing analysis of Brazilian chicken anemia virus isolates that lack MSB-1 cell culture tropism

2007 ◽  
Vol 30 (2) ◽  
pp. 81-96 ◽  
Author(s):  
Eliana Ottati Nogueira ◽  
Antonio J Piantino Ferreira ◽  
Rodrigo Martins Soares ◽  
Edison Luiz Durigon ◽  
Simaia Lazzarin ◽  
...  
Keyword(s):  
Cell Culture ◽  
Genome Sequencing ◽  
Chicken Anemia Virus ◽  
Sequencing Analysis ◽  
Anemia Virus ◽  
Virus Isolates

scholarly journals Pathogenicity and immunosuppressive potential of an Egyptian field isolate (2015) of the chicken anemia virus (CAV) in chickens

2017 ◽  
Vol 24 (1) ◽  
pp. 103-113
Author(s):  
Nassif S.A. ◽  
Anhar A. Abdel Latif ◽  
Nermeen M. Elsayed ◽  
Hayam Farouk ◽  
Ekram Salama ◽  
...  
Keyword(s):  
Field Isolate ◽  
Chicken Anemia Virus ◽  
Anemia Virus

Full-length infectious clone of an Iranian isolate of chicken anemia virus

Virus Genes ◽  
2016 ◽  
Vol 53 (2) ◽  
pp. 312-316 ◽  
Author(s):  
Amir Kaffashi ◽  
Fatemeh Eshratabadi ◽  
Abdelhamed Shoushtari
Keyword(s):  
Infectious Clone ◽  
Full Length ◽  
Chicken Anemia Virus ◽  
Iranian Isolate ◽  
Anemia Virus

Development of a Modified Method for Sequencing High G:C Regions in Chicken Anemia Virus Genome

2002 ◽  
Vol 6 (3) ◽  
pp. 229-232 ◽  
Author(s):  
S.M.Z.H. Chowdhury ◽  
A.R. Omar ◽  
I. Aini ◽  
M. Hair-Bejo ◽  
A.A. Jamaluddin ◽  
...  
Keyword(s):  
Virus Genome ◽  
Chicken Anemia Virus ◽  
Modified Method ◽  
Anemia Virus

scholarly journals Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 842
Author(s):  
Hieu Van Dong ◽  
Giang Thi Huong Tran ◽  
Dai Quang Trinh ◽  
Yohei Takeda ◽  
Haruko Ogawa ◽  
...  
Keyword(s):  
Cell Viability ◽  
Persistent Infection ◽  
In Vitro Model ◽  
Neutralizing Antibody ◽  
Chicken Anemia Virus ◽  
Viral Gene ◽  
Vitro Model ◽  
Anemia Virus ◽  
Cells Cultured

Persistent infection of chicken anemia virus (CAV) in chickens has been suspected to result in immunosuppression and exogenous virus contamination within vaccine production. However, no direct evidence for persistent CAV infection has thus far been obtained. In this study, we aimed to establish an in vitro model of persistent CAV infection. CAV-infected MDCC-MSB1 (MSB1) cells, a Marek’s disease virus-transformed continuous cell line, were cultured in the presence of both CAV and CAV neutralizing antibody (NA). Cell viability, expression of viral antigens, viral DNA, and recovery of CAV were examined by acridine orange/propidium iodide staining, immunofluorescence measurement, real-time PCR, and viral isolation, respectively. The results indicated that CAV was maintained and possibly replicated in CAV-infected cells cultured in the presence of NA, without affecting host cell viability. It was also shown that persistently infectious CAV induced cell death again after removing NA. The persistent infection of CAV in MSB1 cells was not related to viral gene mutation. In summary, we have herein established a novel model of persistent CAV infection in MSB1 cells cultured in the presence of NA.

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