Microscopic and polyclonal antibody-based detection of yellow leaf disease of arecanut (Areca catechuL.)

2011 ◽  
Vol 44 (11) ◽  
pp. 1093-1104
Author(s):  
G. Rajeev ◽  
V. R. Prakash ◽  
M. Mayil Vaganan ◽  
M. Sasikala ◽  
J. J. Solomon ◽  
...  
Keyword(s):  
Polyclonal Antibody ◽  
Yellow Leaf ◽  
Leaf Disease ◽  
Yellow Leaf Disease

scholarly journals Incidence and detection of sugarcane yellow leaf disease in the Philippines

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Jay-Vee S. Mendoza ◽  
Marita S. Pinili ◽  
Fe M. Dela Cueva
Keyword(s):  
The Philippines ◽  
Yellow Leaf ◽  
Leaf Disease ◽  
Yellow Leaf Disease

Regeneration and evaluation of somaclones of sugarcane variety Co86032 for yellow leaf disease resistance and yield traits

2019 ◽  
Vol 44 (2) ◽  
Author(s):  
Praveen Kona ◽  
M Hemanth Kumar ◽  
K H P Reddy ◽  
T M Hemalatha ◽  
D M Reddy ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Yield Traits ◽  
Yellow Leaf ◽  
Sugarcane Variety ◽  
Leaf Disease ◽  
Yellow Leaf Disease

Taqman quantitative PCR for detection of Indian arecanut yellow leaf disease phytoplasma

2015 ◽  
Vol 5 (2) ◽  
pp. 113
Author(s):  
Smita Nair ◽  
Ramaswamy Manimekalai ◽  
Soumya Vadakke Purayil ◽  
Govind P. Rao
Keyword(s):  
Quantitative Pcr ◽  
Yellow Leaf ◽  
Leaf Disease ◽  
Yellow Leaf Disease

Impact of the viruses associated with mosaic and yellow leaf disease on varietal degeneration in sugarcane

2019 ◽  
Vol 47 (4) ◽  
pp. 591-604 ◽  
Author(s):  
K. Bagyalakshmi ◽  
R. Viswanathan ◽  
V. Ravichandran
Keyword(s):  
Yellow Leaf ◽  
Leaf Disease ◽  
Yellow Leaf Disease

scholarly journals Screening and characterisation of virus causing yellow leaf disease of Tephrosia in Ethiopia

2020 ◽  
Vol 49 (4) ◽  
pp. 447-450
Author(s):  
Alok Kumar ◽  
Jean Hanson ◽  
Chris S. Jones ◽  
Yilikal Assefa ◽  
Fikerte Mulatu
Keyword(s):  
Yellow Leaf ◽  
Leaf Disease ◽  
Yellow Leaf Disease

scholarly journals Prevalence of Yellow Leaf Disease (YLD) and its Associated Areca Palm Velarivirus 1 (APV1) in Betel Palm (Areca catechu) Plantations in Hainan, China

Plant Disease ◽  
2020 ◽  
Vol 104 (10) ◽  
pp. 2556-2562
Author(s):  
Hongxing Wang ◽  
Ruibai Zhao ◽  
Huaiwen Zhang ◽  
Xianmei Cao ◽  
Zhaotong Li ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Strong Association ◽  
Yellow Leaf ◽  
Leaf Disease ◽  
Transmission Electron ◽  
Areca Catechu ◽  
Yellow Leaf Disease ◽  
Typical Morphology ◽  
Generation Sequencing ◽  
Important Disease

Yellow leaf disease (YLD) is an economically important disease affecting betel palm in several countries, the cause of which remains unclear despite associations with putative agents, including phytoplasmas. In this study, we screened the potential casual agents associated with YLD in Hainan, China using next-generation sequencing and revealed the association of areca palm velarivirus 1 (APV1) with the YLD-affected palm. The complete genome of the APV1-WNY isolate was determined to be 17,546 nucleotides in length, approximately 1.5 kb longer than the previously reported APV1_HN genome. Transmission electron microscopy showed that APV1 particles are flexuous and filamentous, a typical morphology of species in the Closteroviridae family. Comparison of symptomatic and symptomless tree populations showed a strong association between APV1 and YLD. APV1 was detected in Pseudococcus sp. mealybugs sampled from YLD-affected trees in many locations, suggesting that mealybugs are a potential transmission vector for APV1. Although further studies are needed to confirm a causal relationship, these results provide timely information for the prevention and management of YLD associated with APV1.

Phylogenetic analysis identifies a ‘Candidatus Phytoplasma oryzae ’-related strain associated with yellow leaf disease of areca palm (Areca catechu L.) in India

2013 ◽  
Vol 63 (Pt_4) ◽  
pp. 1376-1382 ◽  
Author(s):  
Manimekalai Ramaswamy ◽  
Smita Nair ◽  
V. P. Soumya ◽  
George V. Thomas
Keyword(s):  
16S Rrna ◽  
Type Species ◽  
Rrna Gene ◽  
Yellow Leaf ◽  
Content Type ◽  
Link Type ◽  
Leaf Disease ◽  
White Leaf ◽  
Yellow Leaf Disease ◽  
The 16S Rrna Gene

Yellow leaf disease (YLD) with phytoplasmal aetiology is a serious disease of arecanut palm in India. The present study was undertaken to characterize the 16S rRNA and secA gene sequences of the Indian arecanut YLD phytoplasma for ‘Candidatus Phytoplasma ’ species assignment and 16Sr group/subgroup classification. Phytoplasma 16S rRNA genes were amplified using three sets of semi-nested/nested primers, 1F7/7R3–1F7/7R2, 4Fwd/3Rev–4Fwd/5Rev and P1/P7–R16F2n/R16R2, producing amplicons of 491, 1150 and 1250 bp, respectively, from diseased samples. The amplicons were cloned and sequenced. A blast search showed that the sequences had 99 % similarity with sugar cane white leaf phytoplasma (16SrXI) and Napier grass stunt phytoplasma (16SrXI). Phylogenetic analysis based on the 16S rRNA gene revealed the clustering of YLD phytoplasma with the rice yellow dwarf and Bermuda grass white leaf groups. The YLD phytoplasma F2nR2 sequence shared 97.5 % identity with that of ‘Candidatus Phytoplasma oryzae ’ and 97.8 % identity with that of ‘Candidatus Phytoplasma cynodontis ’. Hence, for finer differentiation, we examined the secA gene-based phylogeny, where the YLD phytoplasma clustered with Napier grass stunt and sugar cane grassy shoot phytoplasmas, both belonging to the rice yellow dwarf group. Hence, we are assigning the Indian arecanut YLD phytoplasma as a ‘Candidatus Phytoplasma oryzae ’-related strain. Virtual RFLP analysis of a 1.2 kb fragment of the 16S rRNA gene (F2nR2 region) identified the Indian arecanut YLD phytoplasma as a member of 16SrXI-B subgroup. We name the phytoplasma Indian yellow leaf disease phytoplasma, to differentiate it from the Hainan YLD phytoplasma, which belongs to group 16SrI.

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