Nematicidal activity of tirotundin and parthenolide isolated from Tithonia diversifolia and Chrysanthemum parthenium

2022 ◽  
pp. 1-8
Author(s):  
Mingxian Lan ◽  
Xi Gao ◽  
Xiuan Duan ◽  
Hongmei Li ◽  
Hang Yu ◽  
...  
Keyword(s):  
Nematicidal Activity ◽  
Tithonia Diversifolia

scholarly journals Aktivitas Nematisidal Daun, Batang, dan Bunga Tithonia diversifolia terhadap Nematoda Puru Akar Meloidogyne incognita secara in vitro

2017 ◽  
Author(s):  
Muhammad Firdaus Oktafiyanto ◽  
Ankardiansyah Pandu Pradana ◽  
Abdul Munif
Keyword(s):  
Meloidogyne Incognita ◽  
Environmentally Friendly ◽  
Nematicidal Activity ◽  
Alternative Solution ◽  
Tithonia Diversifolia ◽  
Root Knot Nematode ◽  
Effective Technique ◽  
New Information ◽  
Plantation Crops

Root-knot nematode Meloidogyne incognita is a soil-borne pathogen in plantation crops. Tea, coffee, pepper, tobacco, and patchouli plant have been reported as host of this nematode. Environmentally friendly, inexpensive, and effective technique is required to control the population of M. incognita. Utilization of tithonia plant as botanical nematicide is an alternative solution needs to be applied. A total of 1:10 (w/v) of leaves, stems, and flowers of tithonia was boiled. Further, the decoction was used in the in vitro mortality test of J2 of M. incognita. The result showed that decoction of leaves, stems, and flowers of tithonia at various concentrations led to nematicidal effect against M. incognita. Best performance of nematicidal activity was found in the decoction of flowers at a concentration of 50%. This study provides new information concerning nematicidal effect of decoction of leaves, stems, and flowers of tithonia against M. incognita.

Chemical constituent analysis of EtOAc fraction of Tithonia diversifolia(HEMSL.) A.Gray

2010 ◽  
Vol 30 (2) ◽  
pp. 189-192 ◽  
Author(s):  
Gui-jun ZHAO ◽  
Chong-xi ZHANG ◽  
Zhi-jun WU ◽  
Xia LI ◽  
Ying-bo YANG ◽  
...  
Keyword(s):  
Chemical Constituent ◽  
Tithonia Diversifolia ◽  
Etoac Fraction

Enhanced accumulation of pinosylvin stilbenes and related gene expression in Pinus strobus after infection of pine wood nematode

2021 ◽  
Author(s):  
Hwan-Su Hwang ◽  
Jung Yeon Han ◽  
Yong Eui Choi
Keyword(s):  
Transcription Factors ◽  
Defense Mechanisms ◽  
De Novo ◽  
Pinus Strobus ◽  
Pine Wilt Disease ◽  
Monomethyl Ether ◽  
Bursaphelenchus Xylophilus ◽  
Nematicidal Activity ◽  
Dependent Manner ◽  
Pine Wood

Abstract Pine wood nematodes (PWNs: Bursaphelenchus xylophilus) infect pine trees and cause serious pine wilt disease. Eastern white pine (Pinus strobus) has resistance to PWN. However, the detailed defense mechanisms of P. strobus against PWN are not well known. When P. strobus plants were infected with PWNs, the accumulation of stilbenoids, dihydropinosylvin monomethyl ether (DPME) and pinosylvin monomethyl ether (PME), were increased remarkably. DPME and PME had the high nematicidal activity. Interestingly, the nematicidal activity of the two compounds was resulted in a developmental stage-dependent manner. PME was more toxic to adult PWNs than juveniles, whereas DPME was found more toxic to juvenile PWNs than the adults. The genes involved in PME and DPME biosynthesis such as phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase (4CL), pinosylvin synthase (STS), and pinosylvin O-methyltransferase (PMT) were isolated using de novo sequencing of the transcriptome in P. strobus. In addition, transcription factors (bHLH, MYB and WRKY) related to stilbene biosynthesis were isolated. qPCR analyses of the selected genes (PAL, 4CL, STS, and PMT) including transcription factors (bHLH, MYB and WRKY) revealed that the expression level of the selected genes highly enhanced after PWN infection. Our results suggest that pinosylvin-type stilbenoid biosynthesis is highly responsive to PWN infection and plays an important role in PWN resistance of P. strobus trees.

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