MicroRNA Expression in Neonates with Late-onset Sepsis – A Cross-sectional Comparative Study

2022 ◽  
pp. 1-13
Author(s):  
Saranya Sankar ◽  
Kathirvel Maruthai ◽  
Zachariah Bobby ◽  
Bethou Adhisivam
Keyword(s):  
Comparative Study ◽  
Late Onset ◽  
Microrna Expression ◽  
Cross Sectional ◽  
Late Onset Sepsis

scholarly journals Urinary tract infection among neonatal sepsis of late-onset in Cipto Mangunkusumo Hospital

2016 ◽  
Vol 45 (5) ◽  
pp. 217 ◽  
Author(s):  
Novie Amelia ◽  
Idham Amir ◽  
Partini P Trihono
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Neonatal Sepsis ◽  
Urine Culture ◽  
Late Onset ◽  
Preterm Neonates ◽  
Cross Sectional ◽  
Female Ratio ◽  
Late Onset Sepsis

Background Urine culture, as part of a full septic work-up forlate-onset neonatal sepsis, was not routinely done in the Neona-tal Ward at Cipto Mangunkusumo Hospital, and as of today, theprevalence of urinary tract infection (UTI) among neonates withlate-onset sepsis remains unknown.Objectives To determine the prevalence and microbiological pat-terns of UTI among late-onset neonatal sepsis in CiptoMangunkusumo Hospital.Methods We conducted a cross-sectional study on all neonatesdiagnosed as suspected late-onset sepsis who underwent sep-sis evaluation between 20 October 2003 – 30 April 2004. Urinespecimens were collected by bladder catheterization for cultureand urinalysis.Results UTI was found in 14.9% (7/47) neonates who under-went urine culture (male: female ratio was 5:2). Six subjectswho had UTI were preterm neonates, Klebsiella pneumoniaewas found in both blood and urine cultures of 1 subject, while theothers showed different microorganisms. Forty-five out of 47subjects, who were suspected of late-onset sepsis, had posi-tive blood cultures. All subjects with UTI had positive bacteriuriafrom Gram-stained specimen.Conclusions The prevalence of UTI among neonates with late-onset sepsis in Cipto Mangunkusumo Hospital was 14.9%. Themicroorganisms most frequently found in urine cultures werePseudomonas sp., Staphylococcus epidermidis, and Klebsiellapneumoniae. Urine culture, urinalysis, and urinary Gram-stainshould be performed as part of sepsis evaluation for late-onsetneonatal sepsis, especially in male and preterm neonates

scholarly journals ASSESSMENT OF SERUM PROCALCITONIN LEVELAS A MARKER TO DIAGNOSE LATE ONSET SEPSIS IN NEONATES

2020 ◽  
pp. 1-2
Author(s):  
Javeri Aarti Harish ◽  
Jagadeeswari Jagadeeswari
Keyword(s):  
Neonatal Intensive Care ◽  
Late Onset ◽  
Cross Sectional Study ◽  
Coagulase Negative Staphylococci ◽  
Efficient Tool ◽  
Cross Sectional ◽  
Medical College ◽  
Late Onset Sepsis ◽  
Detection And Diagnosis ◽  
Single Laboratory

Introduction: Sepsis constitutes one of the most prevalent cause of mortality in newborns, eminently in developing countries. There are several investigations available that may be used as indicators to identify sepsis, however, the inability of a single laboratory investigation for rapid detection and diagnosis of sepsis, calls for the need of a specific investigation. Lately, it has been proclaimed that procalcitonin can be used as a reliable predictor for diagnosis and to determine severity as well as outcome of neonatal sepsis. Objective: To assess levels of serum Procalcitonin level as a marker to diagnose late-onset sepsis in a neonatal intensive care unit (NICU). Methods and Materials: A cross-sectional study was performed between the June 2018 to November 2018 at Sree Balaji Medical College and Hospital. Serum procalcitonin levels were determined for 25 neonates of age between 3-30 days. Results: Distinct elevations of procalcitonin levels were observed in neonates with late-onset sepsis caused mainly by coagulase-negative staphylococci. Currently nosocomial infection due to coagulase-negative staphylococci is a frequent occurrence in NICUs. Conclusion: Serum procalcitonin is an efficient tool to diagnose Late Onset of Sepsis.

scholarly journals Isolation and Identification of Serratia marcescens from Suspected Late Neonatal Sepsis in Intensive Care Unit

2018 ◽  
Vol 9 (01) ◽  
Author(s):  
Jabbar S. Hassan ◽  
Ahmed E. Salman ◽  
Ahmed S. Obeid ◽  
Thana R. Abdul Rhman
Keyword(s):  
Serratia Marcescens ◽  
Neonatal Sepsis ◽  
Late Onset ◽  
Venous Blood ◽  
Cross Sectional Study ◽  
Molecular Technique ◽  
Luxs Gene ◽  
Isolation And Identification ◽  
Cross Sectional ◽  
Late Onset Sepsis

Background: Neonatal sepsis stays one of the main sources of morbidity and mortality both among infant in ICU, in light of the planning of the disease neonatal sepsis has been categorized into early and late-onset sepsis, where the latter occurs after one week of life and is often more insidious in onset than the former. Objective: To detect the rate of Serratia marcescens infection in neonatal sepsis in ICU by molecular technique. Methods: A total of 50 neonates with the age group 8 days to 30 days who were admitted to AL-Kadhumiya Teaching Hospital/ Baghdad during the period January to March ,2017 were recruited for this cross-sectional study. Approximately 3 ml of venous blood were obtained from each patients. These samples were examined for septicemia by blood culturing followed by API20 for quick identification of relevant bacteria. Furthermore, bacteria DNA was isolated directly from blood samples, and conventional PCR based on luxS gene, highly specific to S. marcescens, was achieved. Results: Blood culture were positive in 36(72 %) out of 50 samples; the most common bacterial causes were Staphylococcus aureus (18.7%), Pseudomonas aeruginosa (13.8%) and Serratia marcescens (11.1%), Molecular method revealed specific amplification of luxS gene in 12 samples (24%). Conclusion: Serratia marcescens has risen as a most widely recognized causative agent in late onset sepsis.

scholarly journals Characteristics of neonatal late onset sepsis in Sanglah Hospital, Bali

2021 ◽  
Vol 7 (2) ◽  
pp. 073-082
Author(s):  
Arya Wisnu Prayoga ◽  
Made Kardana ◽  
I Wayan Dharma Artana ◽  
Putu Junara Putra ◽  
Made Sukmawati
Keyword(s):  
Gestational Age ◽  
Neonatal Intensive Care ◽  
Late Onset ◽  
Laboratory Finding ◽  
Cross Sectional ◽  
Microbial Characteristics ◽  
Level Ii ◽  
Late Onset Sepsis ◽  
Poor Outcome Group ◽  
Outcome Group

Neonatal Late-Onset Sepsis (LOS) is a leading cause of mortality in the Neonatal Intensive Care Unit (NICU). The microbial characteristics of LOS are of primary importance in guiding clinical antisepsis practice, and strategies to prevent and treat neonatal LOS, in turn, influence the pattern of LOS pathogens. This study is a retrospective descriptive study with a cross-sectional approach conducted between 2016 until 2020 in the neonatal ward (level II-III) of the Sanglah General Hospital, Bali. Data collected consists of demography, clinical characteristics, laboratory results, and outcomes. Subjects in this study dominated by male (64%), gestational age > 37 weeks (56%), born ≥ 2.500 grams (54%), last mother education mostly in Senior High School (56%), spontaneous delivery method (54%) and 31 (62%) subjects were referral from other hospital and primary health care. Most of the subjects were lethargic (68%) and 15 (30%) subjects were died. Laboratory finding normal leukocyte, neutrophil, lymphocyte, hemoglobin, thrombocyte and IT ratio but have higher procalcitonin result. Poor outcome group were dominated by male, smaller gestational age, VLBW, and neonates who experience lethargy, temperature instability, respiratory distress and got positive blood culture.

SIGNIFICANCE OF PLACENTAL HISTOPATHOLOGICAL FINDINGS IN PRETERM/TERM BIRTH

2020 ◽  
Vol 4 (5) ◽  
Author(s):  
Monika Kushwaha ◽  
Sanjeev Narang
Keyword(s):  
Preterm Birth ◽  
Comparative Study ◽  
Pregnancy Outcome ◽  
Neonatal Morbidity ◽  
Research Centre ◽  
College Hospital ◽  
Cross Sectional ◽  
Chorionic Villi ◽  
Term Birth ◽  
Medical College

Background: This study is cross-sectional, observational and comparative study, at Index Medical College, Hospital & Research Centre, Indore, Madhya Pradesh from July 2017 to July 2019 with sample size 100 placentae. Method: The placenta received was evaluated blinded of maternal pregnancy outcome. The pattern of morphology was evaluated both qualitatively (type of lesion) and quantitatively (number of lesions). Result: In Present study 79% of the deliveries were term deliveries and 21% were preterm deliveries. On placental macroscopy, placenta weight was significantly low among the neonates of preterm deliveries (370.00±60.49) as compared to term deliveries (440.89±55.22). Preterm placenta had higher number of abnormal placental lesion compared to term pregnancies. Conclusion: The uteroplacental insufficiency defined as placental infarct, fibrosis of chorionic villi, thickening of blood vessels, and poor vascularity of chorionic villi. Placental histopathological lesions are strongly associated with maternal under perfusion and uteroplacental insufficiency. These are the reasons for preterm birth. Thus, knowledge of the etiological factor can be use to reduce maternal and neonatal morbidity and mortility. Keywords: Placenta, Term & Preterm.

NEONATAL SEPSIS AND IDENTIFICATION OF RISK FACTORS: STUDY IN AVBRH HOSPITAL SAWANGI

2019 ◽  
Vol 3 (6) ◽  
Author(s):  
Pramod P. Singhavi
Keyword(s):  
Risk Factors ◽  
Neonatal Sepsis ◽  
Early Onset ◽  
Late Onset ◽  
Signs And Symptoms ◽  
Premature Rupture ◽  
Maternal Risk ◽  
Late Onset Sepsis ◽  
Early Onset Sepsis ◽  
Meconium Stained Amniotic Fluid

Introduction: India has the highest incidence of clinical sepsis i.e.17,000/ 1,00,000 live births. In Neonatal sepsis septicaemia, pneumonia, meningitis, osteomyelitis, arthritis and urinary tract infections can be included. Mortality in the neonatal period each year account for 41% (3.6 million) of all deaths in children under 5 years and most of these deaths occur in low income countries and about one million of these deaths are due to infectious causes including neonatal sepsis, meningitis, and pneumonia. In early onset neonatal sepsis (EOS) Clinical features are non-specific and are inefficient for identifying neonates with early-onset sepsis. Culture results take up to 48 hours and may give false-positive or low-yield results because of the antenatal antibiotic exposure. Reviews of risk factors has been used globally to guide the development of management guidelines for neonatal sepsis, and it is similarly recommended that such evidence be used to inform guideline development for management of neonatal sepsis. Material and Methods: This study was carried out using institution based cross section study . The total number neonates admitted in the hospital in given study period was 644, of which 234 were diagnosed for neonatal sepsis by the treating pediatrician based on the signs and symptoms during admission. The data was collected: Sociodemographic characteristics; maternal information; and neonatal information for neonatal sepsis like neonatal age on admission, sex, gestational age, birth weight, crying immediately at birth, and resuscitation at birth. Results: Out of 644 neonates admitted 234 (36.34%) were diagnosed for neonatal sepsis by the paediatrician based on the signs and symptoms during admission. Of the 234 neonates, 189 (80.77%) infants were in the age range of 0 to 7 days (Early onset sepsis) while 45 (19.23%) were aged between 8 and 28 days (Late onset sepsis). Male to female ratio in our study was 53.8% and 46% respectively. Out of total 126 male neonates 91(72.2%) were having early onset sepsis while 35 (27.8%) were late onset type. Out of total 108 female neonates 89(82.4%) were having early onset sepsis while 19 (17.6%) were late onset type. Maternal risk factors were identified in 103(57.2%) of early onset sepsis cases while in late onset sepsis cases were 11(20.4%). Foul smelling liquor in early onset sepsis and in late onset sepsis was 10(5.56%) and 2 (3.70%) respectively. In early onset sepsis cases maternal UTI, Meconium stained amniotic fluid, Multipara and Premature rupture of membrane was seen in 21(11.67%), 19 (10.56%), 20(11.11%) and 33 (18.33%) cases respectively. In late onset sepsis cases maternal UTI, Meconium stained amniotic fluid, Multipara and Premature rupture of membrane was seen in 2 (3.70%), 1(1.85%), 3 (5.56%) and 3 (5.56%) cases respectively. Conclusion: Maternal risk identification may help in the early identification and empirical antibiotic treatment in neonatal sepsis and thus mortality and morbidity can be reduced.

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